Biomedical subjects

Norman R Heckenberg

Publications and source records attributed to Norman R Heckenberg.

7 recordsLinked to original sources

PubMed2006

Measurement of the index of refraction of single microparticles.

The refractive index of single microparticles is derived from precise measurement and rigorous modeling of the stiffness of a laser trap. We demonstrate the method for particles of four different materials with diameters from 1.6 to 5.2 microm and achieve an accuracy of better than 1%. The method greatly contributes as a new characterization technique because it works best under conditions (small particle size, polydispersion) where other methods, such as absorption spectroscopy, start to fail. Particles need not be transferred to a particular fluid, which prevents particle degradation or alteration common in index matching techniques. Our results also show that advanced modeling of laser traps accurately reproduces experimental reality.

Models, Biological↗

PubMed2006

Optical force field mapping in microdevices.

We present a method for characterizing microscopic optical force fields. Two dimensional vector force maps are generated by measuring the optical force applied to a probe particle for a grid of particle positions. The method is used to map out the force field created by the beam from a lensed fiber inside a liquid filled microdevice. We find transverse gradient forces and axial scattering forces on the order of 2 pN per 10 mW laser power which are constant over a considerable axial range (>35 microm). These findings suggest future useful applications of lensed fibers for particle guiding/sorting. The propulsion of a small particle at a constant velocity of 200 microm s(-1) is shown.

Lasers↗

PubMed2006

Mechanics of cellular adhesion to artificial artery templates.

We are using polymer templates to grow artificial artery grafts in vivo for the replacement of diseased blood vessels. We have previously shown that adhesion of macrophages to the template starts the graft formation. We present a study of the mechanics of macrophage adhesion to these templates on a single cell and single bond level with optical tweezers. For whole cells, in vitro cell adhesion densities decreased significantly from polymer templates polyethylene to silicone to Tygon (167, 135, and 65 cells/mm(2)). These cell densities were correlated with the graft formation success rate (50%, 25%, and 0%). Single-bond rupture forces at a loading rate of 450 pN/s were quantified by adhesion of trapped 2-microm spheres to macrophages. Rupture force distributions were dominated by nonspecific adhesion (forces <40 pN). On polystyrene, preadsorption of fibronectin or presence of serum proteins in the cell medium significantly enhanced adhesion strength from a mean rupture force of 20 pN to 28 pN or 33 pN, respectively. The enhancement of adhesion by fibronectin and serum is additive (mean rupture force of 43 pN). The fraction of specific binding forces in the presence of serum was similar for polystyrene and polymethyl-methacrylate, but specific binding forces were not observed for silica. Again, we found correlation to in vivo experiments, where the density of adherent cells is higher on polystyrene than on silica templates, and can be further enhanced by fibronectin adsorption. These findings show that in vitro adhesion testing can be used for template optimization and to substitute for in-vivo experiments.

Animals↗

PubMed2006

Orientation of optically trapped nonspherical birefringent particles.

While the alignment and rotation of microparticles in optical traps have received increased attention recently, one of the earliest examples has been almost totally neglected--the alignment of particles relative to the beam axis, as opposed to about the beam axis. However, since the alignment torques determine how particles align in a trap, they are directly relevant to practical applications. Lysozyme crystals are an ideal model system to study factors determining the orientation of nonspherical birefringent particles in a trap. Both their size and their aspect ratio can be controlled by the growth parameters, and their regular shape makes computational modeling feasible. We show that both external (shape) and internal (birefringence) anisotropy contribute to the alignment torque. Three-dimensionally trapped elongated objects either align with their long axis parallel or perpendicular to the beam axis depending on their size. The shape-dependent torque can exceed the torque due to birefringence, and can align negative uniaxial particles with their optic axis parallel to the electric field, allowing an application of optical torque about the beam axis.

Birefringence↗

PubMed2005

Characterization of optically driven fluid stress fields with optical tweezers.

We present a controlled stress microviscometer with applications to complex fluids. It generates and measures microscopic fluid velocity fields, based on dual beam optical tweezers. This allows an investigation of bulk viscous properties and local inhomogeneities at the probe particle surface. The accuracy of the method is demonstrated in water. In a complex fluid model (hyaluronic acid), we observe a strong deviation of the flow field from classical behavior. Knowledge of the deviation together with an optical torque measurement is used to determine the bulk viscosity. Furthermore, we model the observed deviation and derive microscopic parameters.

Journal Article↗

PubMed2004

Optical microrheology using rotating laser-trapped particles.

We demonstrate an optical system that can apply and accurately measure the torque exerted by the trapping beam on a rotating birefringent probe particle. This allows the viscosity and surface effects within liquid media to be measured quantitatively on a micron-size scale using a trapped rotating spherical probe particle. We use the system to measure the viscosity inside a prototype cellular structure.

Calcium Carbonate↗

PubMedDate not supplied

Measurement of action spectra of light-activated processes.

We report on a new experimental technique suitable for measurement of light-activated processes, such as fluorophore transport. The usefulness of this technique is derived from its capacity to decouple the imaging and activation processes, allowing fluorescent imaging of fluorophore transport at a convenient activation wavelength. We demonstrate the efficiency of this new technique in determination of the action spectrum of the light mediated transport of rhodamine 123 into the parasitic protozoan Giardia duodenalis.

Animals↗