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Biomedical subjects

O A Aksenov

Publications and source records attributed to O A Aksenov.

At least 19 recordsLinked to original sources
PubMed2005

[Chronic EBV-infection in children with a high level of anti-H+/K+-ATPhase antibodies in gastric parietal].

We examined 50 children at the age of 4-17 suffering from chronic gastritis. AntiH+/K+-ATPhase antibodies of gastric parietal cells, anti-EBV antibodies IgG EA and IgM VCA were determined with the help of immune-enzyme analysis methods. The first group comprised 28 children with a high level of antiparietal antibodies in the blood serum, and 22 children with a normal level of antiparietal antibodies made up the second group. The HP identification was carried out for all children. Anti-EBV antibodies IgG EA were found in 72% of children, and there were no statistical differences in the frequency and level of antibodies IgG EA. IgM VCA were not found in any of the patients. However, HP-infection was revealed in 64.3% in the first group vs. 22.7% in the second group (p < 0,05); the combination of HB and chronic EBV infection was also more frequent in the first group than in the second group (42.8% and 18.1%, respectively, p < 0,05).

Adolescent↗
PubMed1999

[Antiviral activity of poly(A)-poly(U) duplexes modified with cis-diammine-dichloroplatinum (II)].

Polyribonucleotide duplex poly(A).poly(U) was modified with cis-diammine dichloroplatinum (II) (cis-DDP). It was shown that the antiinfluenza protective activity of the modified duplex in mice increased with the degree of modification (rb) rising up to 0.2. The effect was different from that for poly(I).poly(C) and poly(G).poly(C). The interferon titers in the murine brain increased in parallel with increasing of the antiviral activity. It was assumed that the structural specificity of the poly(A).poly(U) duplex was responsible for the phenomenon and that cis-DDP interaction with N(7) atoms of the adenine heterocycles blocked the "abnormal" Hoogsteen pairing of adenines with uracils. As a result the antiviral activity increased because of lowering the quantity of the intramolecular defects and increasing the length of the regular double-stranded regions.

Animals↗
PubMed1999

[The use of a modified complement fixation reaction for the rapid diagnosis of viral infections in children].

A system of diagnostic tests (complement enzyme assay) is developed, detecting viral and other antigens, toxins, antibodies, and specific immune complexes in liquid enzyme immunoassay based on the complement fixation test. The system is simple and economic, the results can be transferred into digital data, and the above factors can be detected individually in biological materials from patients. The system is effective, and in many cases (for example, in the diagnosis of enteroviral diseases) is the only method for rapid isolation and typing of the infection agents.

Child↗
PubMed1999

[Broadening of the organ specificity of the action of polynucleotide interferon inducers].

Interferon titers in the blood and brain of mice and their protection from the herpes virus were compared after the animal exposure to poly(G).poly(C) duplex, both native and modified with cis-diammine dichloroplatinum (II). It was shown that the duplex platination especially at the level of the poly(G) strand resulted in sharp rising of the interferon titers in the extracts of the animal brain and rearrangement of the types of interferon induced in the brain to predominance of gamma-interferon. The interferonogenesis indices correlated with the duplex protective activity against the herpes virus. It was concluded that the platinum binding could increase the membrane specificity of the duplex and stimulate its penetration through the hematoencephalic barrier. Possible structural changes in the duplex under the action of platinum (II) resulting in the observed effect are discussed.

Animals↗
PubMed1998

[Correctional aspects of anisometropia in railway machine operators].

The authors assessed occupationally significant visual functions in anisometropy varying in type and degree. Disorders of occupationally significant visual functions depend reliably on anisometropy degree. Correction of anisometropy with recovered vision and binocular functions restores all parameters of night and color vision. Optic and operative correction of anisometropy could be used for rehabilitation of railway machine operators with long length of service. The article provides recommendations to use intraocular lenses for artiphakia in railway machine operators.

Anisometropia↗
PubMed1994

[The astrocyte glia of the brain in herpetic encephalitis in children].

Astrocytic changes were followed up in the brain of infants who had died of herpetic encephalitis. Neuro-morphological examinations revealed astrocytic structural changes directed to reinforcement of the blood-brain barrier, localization of the infection and, possibly, to inactivation of the virus.

Astrocytes↗
PubMed1993

[Infectious placentitis and characterization of the placenta as an immune barrier].

Analysis of publications dealing with infectious pathology of placenta is performed. Placentitis of different etiology and different routes of contamination have specific features. The incidence of viral and mycoplasma etiology with intracellular multiplication of the agent is considerably higher than of bacterial or other placentitis with extracellular multiplication. The incidence of placentitis is considerably higher than that of intrauterine infection. Placenta is an organ of immune protection of the organism and is capable to produce both specific and non-specific immunity factors.

Bacterial Infections↗
PubMed1992

[Viral and bacterial associations and their role in the development of bronchopulmonary diseases in children].

Clinical and laboratory criteria for estimating the role of viruses and bacteria that determine bronchopulmonary diseases have been derived. The clinical importance of the detectable microflora, part of which permanently invade the nasopharynx (pneumococcus, adenoviruses) is under critical review. Pneumonias that may develop within the first days of acute respiratory viral infection are characterized by monoviral influenzal or RS-infection; later pneumonias are marked by viral infection with the predominance of adenoviruses. Attempt has been made to reveal the role of geno- and phenotypic factors (N-acetylation, lipid peroxidation, synthesis of alpha-interferon). The data obtained support an assumption about self-regulation of the child's immune system and the adaptation character of responses in mixed infections.

Adenovirus Infections, Human↗
PubMed1987

[Involvement of the afterbirth in influenza].

Light- and fluorescence-microscopy examinations of 186 placentas were carried out, with concurrent determination of placental interferon, isolation of influenza virus, and serologic tests of maternal and fetal blood, and amniotic fluid. In 32 of the cases, placentitis caused by serotype A or B of influenza virus was present, characterized by hyperplasia and subsequent destruction of amniotic cells, trophoblast, decidual cells, and vascular endothelium, by the presence of influenza antigens, fuchsinophilic cytoplasmic inclusions in the affected cells, and lymphoid infiltrates, and/or by circulatory disturbances. In addition, an interferon possessing properties of viral, immune, and placental interferon was detected in a number of placentas. A or B influenza virus was isolated from 3 placentas. Diagnostic titers of anti-influenza antibody occurred in fetal blood and amniotic fluid samples. The present results indicate that influenza virus may persist and replicate in placental cells.

Adolescent↗
PubMed1985

[Pathogenetic and pathomorphological problems of viral-bacterial associations in children dying of meningococcal infection].

The information on 63 children dying from hypertoxic forms of meningococcal infection is presented. Four groups of brain damage by respiratory viruses (RV) are distinguished on the basis of the results of morphological and virological examination: 1) with a recent RV generalization (26 cases); 2) with dissemination of an etiological agent but without clear-cut structural changes (6 cases); 3) with an isolated affection of the brain (13 cases); 4) without clear-cut brain damage. Experimental influenza-meningococcal infection was reproduced in 260 white rats. Enhancement of the animal death rate, multiplication of virus and the degree of brain damage in cases of combined action of both etiological agents is demonstrated. The ability of influenza virus, when inoculated intranasally together with meningococcus, to penetrate and to multiply in the brain provoking meningitis and choroiditis is shown virologically, histologically and electron microscopically.

Adenovirus Infections, Human↗
PubMed1983

Extrapulmonary lesions in influenza.

The authors, having investigated 270 autopsied cases of influenza in children, reported the possibility of a hematogenic virus dissemination from the respiratory system. In the acute stage of the disease the antigen of influenza was revealed in the liver, intestine, kidneys, brain and other organs with adequate regularity. The antigen was localized in the epithelial cells and also endothelial, ependymal and some other cells. The latter were partly accompanied by specific structural changes similar to those that occur in the respiratory system in the presence of the same disease. Besides, degenerative, exudate and, now and then, proliferative and regenerative changes which were not specific for influenza, have been found in all affected organs. Of particular interest were the results of brain study, in which viral particles were revealed by electron microscopic examination in chronic inflammatory lesions. In the experimental study carried out on 180 newborn mice by means of histological, immunofluorescent, serological and virological methods was proven the possibility of generalization of influenza, and some aspects of the pathogenesis were discussed.

Animals↗
PubMed1983

[Chronic isolated brain lesions caused by respiratory viruses in children].

Combined study of 48 postmortem observations of cerebral lesions caused mainly by meningococcal infection in children was carried out. In 15 cases, isolated subclinical involvement of the brain (more frequently, of the vascular plexus and meninges) caused by influenza A viruses (8 cases), B (2 cases), adenoviruses (6), parainfluenza and respiratory syncytial virus (1 each), and chlamydia (2) was found. This was proved by the presence of the appropriate antigens in the brain revealed by immunofluorescence which were absent in respiratory organs, high titres of antibody to the same agents in the cerebrospinal fluid which were lacking in 13 cases or had significantly low titres in 2 cases in the blood, virus isolation from the brain (2 cases) and detection of virus particles in 1 out of 2 cases from which the vascular plexus was examined by electron microscopy. Histological examination of the affected areas revealed structural changes of ependymocytes which were principally similar to those described in respiratory organs as well as in other organs in cases of generalization of respiratory infections. Different degrees of sclerosis of vascular plexus (2 cases) or meninges (2 cases) were also observed.

Adolescent↗