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Biomedical subjects

O A Azizova

Publications and source records attributed to O A Azizova.

At least 127 records · Page 7Linked to original sources
PubMedDate not supplied

[Effect of tetracaine on thrombocyte aggregation and mobilization of the intracellular calcium].

Tetracaine (1 mM), a local anesthetics, lowers a degree of aggregation of human thrombocytes which is induced by thrombin (0.15 u/ml) and suppresses its appearance. Aggregation of thrombocytes induced by phorbol ester, TPA (10-8 M), an activator of protein kinase C, is inhibited completely by the mentioned doses of the anesthetics. In the presence of tetracaine the release of intracellular Ca is lower to some extent, but then it surpasses the control level. It is established that under the action of ionophore A23187 tetracaine exerts no effect on mobilization of intracellular Ca2+.

Blood Platelets↗
PubMedDate not supplied

[Reactivation of superoxide dismutase by the helium-neon laser irradiation].

When incubating SOD for two hours in the buffer with pH 6.0 practically complete loss of the activity of enzyme was observed at insignificant changes of the absorption spectra in the region of 450 and 680 nm. Irradiation of SOD solution with helium-neon laser resulted in the dose-dependent reduction of spectral and enzymic properties. Similar effect of the red light was also observed when SOD was inactivated with hydrogen peroxide: laser irradiation brought about the reduction of initial absorption spectrum of the enzyme. One can believe that the therapeutic effect of helium-neon laser radiation is conditioned, at least partially, by photochemical reactivation of SOD inactivated under pathological conditions (inflammation, hypoxia) in the tissues.

Enzyme Activation↗
PubMedDate not supplied

[Thyroid hormones and the level of divalent iron ions in the rabbit liver].

Experimental hypothyreosis in animals was obtained by means of extirpation of thyroid gland; hyperthyreosis was obtained by thyroxine injections intraperitoneally for 10 days. Content of Fe2+ ions of the free pool (not bound with ferritine) was estimated with the ESR method, after treatment with sodium nitrite. A decrease in Fe2+ ions content is observed in liver homogenate of hypothyroid rabbits, and an increase is observed in that of the hyperthyroid animals. Content of Fe2+ ions in cells of liver tissues correlates with the oxidation capacity of the mitochondrial lipids. The changes in the rate of lipid peroxidation under thyroid pathology is supposed to result from changes in free ferrum content.

Animals↗
PubMedDate not supplied

[Study of Ca-dependent ATPase of sarcoplasmic reticulum by the use of selectively bound spin labels].

The dependence of the ESR spectral parameters of spin labelled sarcoplasmic reticulum membranes on the amount of bound maleimide spin label is found which is shown to result from non-equivalence of the different SH groups rather than from enzyme activity changes on modification. Some structural characteristics (local polarity, the accessibility to water soluble reagents, the distance from ATP binding site) of the fast and slowly reacting SH groups were studied using selective labelling technique. The main contribution to the ESR absorption for both types of thiol groups gives the spin labelled Ca-ATPase. The slow rotational mobility of both types of strongly immobilized spin labels is measured using microwave saturation technique. This mobility is shown to be due essentially to intramolecular motion in different regions of the Ca-ATPase. The frequency of this motion correlates with the enzyme activity and it is sensitive also to the interaction of the enzyme with the substrates (ATP, acetylphosphate) and to the changes of lipid-protein interaction by temperature variation.

Animals↗
PubMedDate not supplied

[Thyroid hormones and EPR signals of the liver, brain and blood].

Experimental hypothyreosis was caused by thyroidectomy, hyperthyreosis developed after intraperitoneal administration of thyroxine into animals within 10 days. In liver and brain preparations amplitudes of EPR signals were decreased with g = 2.00 and g = 1.94, respectively, in hypothyreosis and were increased in hyperthyreosis. The ratio of these signals amplitudes was unaltered in brain tissue but in liver tissue it varied depending on thyroid status. In liver tissue intensity of other signals was increased in hyperthyreosis ad decreased in hypothyreosis, except for the signal with g = 2.25 (cytochrome P = 450) the intensity of which decreased in hyperthyreosis and increased in hypothyreosis. These phenomena appear to be relevant to various disorders of metabolism in pathologies of thyroid glands.

Animals↗