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O A Kraĭnova

Publications and source records attributed to O A Kraĭnova.

9 recordsLinked to original sources
PubMedDate not supplied

[Change in the activity of the enzymatic systems of Bacillus anthracoides spores during germination and under the action of Ca hypochlorite].

The activity of the enzymes of the citric acid cycle, glycolysis, and hexose monophosphate pathway was studied during germination of the spores of Bacillus anthracoides and upon their treatment with calcium hypochlorite. No activity of isocitrate dehydrogenase and glucose-6-phosphate dehydrogenase was found in the extracts of the vegetative cells, contrary to the spores and initiated spores. The activity of other enzymes changes but slightly in the course of germination of the spores. Treatment of the spores with calcium hypochlorite inhibited their initiation and germination and the activity of several enzymes, especially malate dehydrogenase, isocitrate dehydrogenase, and fumarase.

Acids↗
PubMedDate not supplied

[Comparative effect of various chloroactive compounds on the enzyme systems of Bacillus anthracoides spores].

The effect of various chloroactive compounds containing equal amounts of chlorine on respiration enzymes was studied in the spores of Bacillus anthracoides 96. These compounds inhibited the activity of malate dehydrogenase, fumarase, and aconitase but stimulated the activity of glucose-6-phosphate dehydrogenase, succinate dehydrogenase, and ketoglutarate dehydrogenase. Therefore, the action of these compounds is rather specific, and the inhibition of the activity of some enzymes is one of the factors causing the sporocidal effect.

Bacillus↗
PubMedDate not supplied

[Amino acid and mineral element content and the activity of various enzymes in germinating spores of Bacillus thuringiensis].

Changes in the content of dipicolinic acid and mineral elements were studied in the process of Bacillus thuringiensis spore germination. The spores released up to 28% of dipicolinic acid and 18% of calcium at the activation stage, and 93 and 91%, respectively, at the initiation stage. At the same time, the content of Mg, Mn, Zn and P decreased while K, Na and Fe accumulated in the spores. The activities of total and serine proteases, alkaline phosphatase, NADH dehydrogenase and aldolase increased in the extract of initiated spores. The content of glutamate decreased in the free amino acid pool as early as by the 30th second of the initiation stage.

Alkaline Phosphatase↗
PubMedDate not supplied

[Variant of Bacillus anthracoides].

A comparative study of the Bacillus anthracoides culture and its variant has shown that the latter differs drastically from the parent culture in the shape and consistence of colonies, the size of spores and vegetative cells, the rate of spore germination in MPB, and the resistence to steam treatment and chloroactive disinfectants.

Bacillus↗
PubMedDate not supplied

[Activity of intracellular enzymes in Bacillus thuringiensis prospores and sporangia].

The activity of endocellular enzymes (alkaline phosphatase, protease, glucose dehydrogenase, aldolase, malate dehydrogenase, NADH dehydrogenase, NADH oxidase) was studied in isolated prospores and sporangia as well as in vegetative cells of Bacillus thuringiensis strains, one of which produced crystals and one did not. The activity of malate dehydrogenase and NADH dehydrogenase was high in prospores of the both strains at the fifth and sixth stages of spore formation. The strain which did not produce crystals differed from the parent strain by a higher aldolase activity at all of the growth stages and by an abrupt increase in the activity of hydrolytic enzymes in sporangia (in the cytoplasm of the parent cells).

Alkaline Phosphatase↗