Biomedical subjects
Publications and source records attributed to O A Panteleev.
The comparative study of several batches of conjugates has revealed that enzyme immunoassay techniques can be used for the standardization of conjugates by their affinity level. The study has shown that this can be done only if the concentration of specific antibodies in the conjugate is known and the amount of the conjugate is in excess to that of the antigen adsorbed on the plate.
The problems of selecting the concentration of reagents with the aim of increasing the accuracy and sensitivity of reactions, as well as decreasing the consumption of reagents, are shown as exemplified by the antigen-antibody reaction of the first order. A new approach to the ELISA reaction is proposed, which makes it possible to present the totality of consecutive interactions of the reagents as block diagrams described by known mathematical expressions. The limitations of the linear dependence of the results of the reaction are shown, and the methodological recommendations for overcoming these limitations are given.
The study of the accuracy of the enzyme-linked immunosorbent assay has revealed that the accuracy of this assay is low. The influence of instruments (dispensers, photometers, plates) on the accuracy of the assay has been studied. As shown in this study, the main sources are titration procedures and differences in the adsorption and optical properties of available plates, those manufactured in the USSR giving greater errors.
The data indicating that the degree of sorption of the antigen onto the plate depends on the duration of this process are presented. As shown in this investigation, the lower the adsorption activity of the plates, the longer the process of the sensitization of plates with the antigen. The process of the sorption of the antigen onto the plates with low adsorption capacity may be accelerated by sensitizing the plates at different temperatures. Besides, the adsorption capacity of the plates was increased by ultraviolet irradiation.