[Burnett's syndrome and urolithiasis in peptic ulcer].
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Diagnosis, Differential↗
Biomedical subjects
O A Shul'ga
Publications and source records attributed to O A Shul'ga.
[Determination of type and spatial pattern formation of flower organs: dynamic model of development].
The mathematical model imitating floral organ spatial pattern formation (positioning) was developed. Computer experiments performed on its basis demonstrated that organ spatial pattern formation in typical crucifer flower occurred in successive order: medial sepals, carpels, lateral sepals, long stamens, petals and short stamens. The positioning was advanced in two directions, acropetally in the perianth and basipetally in the stamens and carpels. The organ type specifying and positioning take place non-simultaneously in different floral areas. The organ type specifying passed ahead of organ primordial spatial pattern formation. The modeling of flower development of several mutants demonstrated that arabidopsis genes AP2 and AG in addition to specifying floral organ types also determine the particular zones in the floral meristem for futur organ development. The AG gene controls the formation of basipetal patterning zones where the reproductive organs develop, AP2 maintains the proliferative activity in the floral meristem that form acropetal patterning zones where perianth organ develop.
Arabidopsis↗
[The effect of the 5'-leader of potato X-virus on expression of the gene for the potato Y virus membrane protein in transgenic Solanum tuberosum].
The 5'-leader of potato virus X (PVX) genomic RNA was successfully used to generate potato plants expressing the coat protein of the potato virus YN (Russian isolate) (PVY CP). Two expression cassettes were constructed that carried the PVY CP coding sequence along with the PVY 3'untranslated region under the control of the CaMV 35S promoter and poly(A) signal. The cassettes contained different sequences between the 35S promoter and the CP artificial AUG initiating codon of the coat protein: either a polylinker or a shortened variant of the PVX leader. Transgenic Belorusskii-3 potato plants generated by agrobacterial transformation were shown to express the PVY CP gene to different extents depending on the cassette integrated into the plant genome. The cassette containing the PVX leader provided detectable amounts of the coat protein in all tested plants, whereas plants carrying the cassette with the polylinker leader produced no coat protein above the Western detection threshold. Northern analysis revealed that the presence of the PVX leader in front of the PVY CP gene initiating codon resulted in a 10-fold increase in the PVY CP mRNA steady-state levels, thus suggesting an influence of the PVX leader on mRNA stability.
Base Sequence↗
[Expression of a partially modified delta-endotoxin gene from Bacillus thuringiensis var. tenebrionis in transgenic potato plants].
A modified gene (Bt77) of delta-endotoxin from Bacillus thuringiensis var. tenebrionis was constructed and cloned into pMON505. This binary transformation vector was introduced into Agrobacterium tumefaciens strains containing different helper disarmed Ti-plasmids, LBA4404, A281, and CBE21. These Agrobacterium strains were used to transform potato stem segments (S. tuberosum, cv Desiree, Resy, Temp, Granat). Regenerants were selected on kanamycin-containing media. The presence of the Bt77 sequence in plant genomic DNA was confirmed by PCR analysis. Bt gene expression was studied in regenerated plants. Western blot analysis revealed that transgenic plants produced the Bt protein in the range of 0.005-0.02% of total protein. Total protection against insect damage of leaf tissue from these plants was observed in laboratory bioassays with of Colorado beetle larvae. Transgenic plants showed incomplete protection from CB larvae.
Agrobacterium tumefaciens↗
[Creation of transgenic plants Nicotiana tabacum and Solanum tuberosum, resistant to the herbicide phosphinothricin].
The expression cassette for phosphinothricin acetyltransferase gene (PAT or bar) from S. hygroscopicus has been constructed on the basis of the pBI121 plasmid. Leaf disks of N. tabacum cv. SR1 and steam segments of S. tuberosum cv. Prigozhii-2 have been transformed using Agrobacterium. Selection of the transformed plants carried out by PCR analysis revealed 12 transgenic tobacco plants and 3 transgenic potato plants. bar gene expression and plant resistance to phosphinothricin treatment have been studied in regenerated plants. All the transgenic plants were found to be resistant to the herbicide. Mendelian inheritance of the inserted gene has been proved by analysis of the F1 progeny of transformed tobacco plants.
Acetyltransferases↗
[Testing transgenic plants using the polymerase chain reaction].
A test system for selecting transgenic plants based on polymerase chain reaction (PCR) has been proposed. It is applicable to primary screening of transgenic plants obtained by cocultivation with Agrobacterium which contains any vector carrying neomycin phosphotransferase genes from transposon Tn5 and Streptococcus (for example pBIN19). These genes confer kanamycin resistance in plants and bacteria respectively. The absence of strong homology between these two genes allows one to perform two PCRs in the same reaction mixture. Thus simultaneous selection of transgenic plants and test for contamination with Agrobacterium are possible. We have also proposed a simple procedure for preparing small samples of plant DNA suitable for PCR detection.
Base Sequence↗