Biomedical subjects
Publications and source records attributed to O A Timasheva.
For the first time an outbreak of ornithosis at a textile factory is described. The data on the specific epidemiological features of the outbreak, the specific clinical features of the disease, the results of catamnestic observations and the study of the titers of specific antibodies in the complement fixation test are presented.
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The possibility of using, on principle, the immunofluorescent microagglutination test with Chlamydia fluorescent corpuscular antigen for the strain-specific differentiation of immune response induced by the causative agents of ornithosis (strain B), enzootic abortus ovis (strain EAE) and lymphogranuloma venereum (strain LV) has been experimentally demonstrated. The calculation of indices, characterizing the specificity of differences between the systems under comparison, by the method of S. Frasser and D. Berman (1965) has confirmed the significance of such differentiation.
The possibility of using the complement fixation test and, mainly, the indirect hemagglutination test with the use of group-specific diagnostic reagents for the tentative serological detection of urogenital chlamydiasis has been studied. The indirect hemagglutination test has proved to be sufficiently informative. Further investigation aimed at the study of the sensitivity and specificity of this test in urogenital chlamydiasis are believed to be expedient and promising.
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In both experimental and clinical conditions the passive hemagglutination test (PHAT with the use of an ornithosis erythrocytic diagnostic preparation was found to be sufficiently sensitive and specific as compared with the complement fixation test (CFT), a routine testing method. The study of the dynamics of immune response in infected animals and ornithosis patients allowed to regard the PHAT as a comparatively early method of serological analysis. Hemagglutinins were also found to circulate in the patients' blood sera only for a short time (on the average for 1 1/2--2 months). The CFT and the PHAT with erythrocytic diagnostic preparation, used in combination, will make it possible not only to diagnose ornithosis in patient more effectively, but also to differentiate between the cases of infection and anamnestic reaction.20
A method of obtaining the fluorescent ornithosis corpuscular diagnostic agent providing for the extraction of the corpuscular antigen with its subsequent conjugation with fluorochrome-fluoresceine isothiocyanate. The use of this preparation permits to stage the agglutination reaction on the basis of immunofluorescent analysis, this facilitating the recording of the reaction results and considerably decreasing consumption of the diagnostic agent. As shown, the suggested immunofluorescent microagglutination reaction was characterised by a sufficiently high sensitivity, specificity, and can be used for detection of antibodies to the causative agent of ornithosis.
An original preparation--dry ornithosis erythrocytic diagnostic agent for the indirect hemagglutination test was prepared on the basis of formalinized tannin-treated sheep red blood cells and group-specific phospholipid antigen of the causative agent of ornithosis. This diagnostic agent retained its specific activity for 18 months (observation period). The use of this preparation considerably facilitated the method of performance of this test, this offering a possibility of its wide application in practice. A sufficiently high sensitivity and specificity of the indirect hemagglutination test with the suggested diagnostic agent, in comparison with the complement fixation test was demonstrated.
A possibility was shown of using the phospholipid group-specific antigen of the ornitosis causative agent in the capacity of a hemosensitin for conducting the indirect hemagglutination test (IHAT). There was revealed a direct proportional relationship between the sensitizing activity and the complement fixing properties of the antigens obtained. A method of sorption of this antigen on the tannin-treated erythrocytes and the optimal parameters of conducting the test with it were elaborated. A sufficiently high sensitivity and specificity of the IHAT was revealed as a result of parallel titration of hetero-and homologous sera in the complement fixation test and in the IHAT. The suggested test can be successfully used for the diagnosis of ornithosis.
A microorganism was isolated from Gamasoid mites (Ornithonyssus sylviarum) collected in nests of blue rock pigeons by means of blind passages in white mice and chick embryos. The studies of its morphological, cultural, and antigenic properties and pathogenicity for laboratory animals permitted to identify it as the causative agent of ornithosis. It is suggested that the possibility of infection of ectoparasites is determined to a considerable extent by the degree of activity of the infectious process in the hosts. Ornithonyssus sylviarum may be assumed to be one of additional reservoirs of the causative agent of ornithosis in nature.
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