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O Constantinescu

Publications and source records attributed to O Constantinescu.

15 recordsLinked to original sources
PubMed1980

Peculiarities of the virus-host cell relationship in a calf kidney cell line persistently infected with measles virus.

Persistent infection with measles virus established in a calf kidney cell line differed in some features from other measles virus carrier systems. After a variable life span, ranging from 50 to 1100 days, six of the chronically infected cell lines showed an evolution towards lytic infection. Only one of these lines, the K-2 cell line, exhibited marked morphological, growth and chromosomal alterations suggesting in vitro cellular transformation. The transformed K-2 cells released infectious measles virus with several modified biological parameters. Persistent infection was not due to the accumulation of thermosensitive mutants or defective particles. The role of the host-cell factor and of the selection of the virus population during its passage in RV cells are discussed.

Animals↗
PubMedDate not supplied

Investigations in the transforming activity of temperature-sensitive mutants of herpes simplex virus.

Transformation of rat embryo fibroblast was induced by a temperature-sensitive mutant of herpes simplex virus type 2. Transformation was scored using the morphological criterion of focus formation and the cell changes that lead to the final focus are described. Virus genome persistence in the transformed cell line was demonstrated by the presence of virus-specific membrane antigens and by the specificity of antibodies elicited in rabbits by inoculation of transformed cells. The cytogenetic analysis of the transformed cells revealed changes in chromosome number and the presence of marker chromosomes.

Animals↗