PubMed Health⌕ Search

Biomedical subjects

O Creach

Publications and source records attributed to O Creach.

At least 19 recordsLinked to original sources

Studies on the hexose region of the lipopolysaccharide from a low virulence strain of Salmonella typhimurium.

The structure of the hexose region of the lipopolysaccharide from M206 strain, a mutant of Salmonella typhimurium having reduced virulence, was partially determined. Immunological tests indicated cross-reactions of anti-(M206) antiserum with wild-type C5 and Ra mutant strains. Data obtained on chemical composition, periodate oxidation, acetolysis, methylation and analysis by gas chromatography/mass spectrometry show that M206 type lipopolysaccharide contains the common core polysaccharide of Salmonella which was substituted in position 4 of the subterminal glucose unit by a disaccharide: D-glucosyl 1----3 D-galactose. This substitution is probably related to the slight virulence of M206 strain.

Acetates↗

[Effect of temperature on diacetoxyscirpenol production and on sporulation in a liquid culture of Fusarium sambucinum].

F. sambucinum grown for 14 days on peptone supplemented Czapek medium produced higher amount of diacetoxyscirpenol at + 15 degrees C than at + 25 degrees C or at temperature kept successively at + 15 degrees C (day) and + 5 degrees C (night). There was no correlation between the spores number and the toxin level, but toxin synthesis began only with sporification and then continued until the 14th day.

Fusarium↗

[Experimental infection of mice by Salmonella typhi inoculated orally: influence of immunosuppressive therapy].

Various immunosuppressive treatments were used in order to induce a Salmonella typhi infection in mouse by oral route. Neither low protein diets nor administration of mucin, iron, streptomycin or silica could modify animal's sensitiveness, but, cyclophosphamide injections enhanced lethal effect of bacteria, while silica increased the number of bacteria in the blood and the spleen.

Administration, Oral↗

[Evolution of mouse intestinal and blood immunoglobulins after oral route vaccination].

The authors have studied the mice immunoglobulins level after vaccination by oral route with a killed-pathogenic strain of Salmonella typhimurium and an avirulent mutant of the same bacteria. The obtained results show an increase of the intestinal IgA and IgG1 levels and a slighter one of sera IgG between the 10 th and 30 th day following immunization. No correlation was observed concerning the IgM, IgG and IgA levels and the mice protection against a challenge of pathogenic bacteria.

Administration, Oral↗

Compared efficiency of a killed vaccine of Salmonella typhimurium, a live vaccine and an antigenic fraction in oral immunization of mice.

In an attempt to determine the best method of immunizing mice by oral route against Salmonella typhimurium, the animals were vaccinated by means of a killed-pathogenic strain, an avirulent mutant of the same bacteria, and a protective antigen removed from the supernatant culture of this mutant. After changing doses of vaccines and the interval between vaccination and challenge, the results obtained show the superiority of the live vaccine and protective antigen with regard to the killed one. Under our experimental conditions enteral vaccination appears to be more effective than the parenteral one.

Administration, Oral↗