Biomedical subjects

O Doyle

Publications and source records attributed to O Doyle.

15 recordsLinked to original sources

PubMed2002

Collaborative investigation of an outbreak of Salmonella enterica serotype Newport in England and Wales in 2001 associated with ready-to-eat salad vegetables.

In June 2001, as part of a microbiological study of bagged, ready-to-eat salad products, Salmonella enterica serotype Newport was isolated from a sample of pre-packed green salad distributed by a major supermarket retailer. The strain was characterised by phage typing, plasmid profile typing and pulsed-field gel electrophoresis. Other isolates of S. Newport from cases of human infection in England and Wales in the first six months of 2001 were similarly characterised. Of 60 strains from cases of human infection, 19 were found to be indistinguishable from that isolated from the salad product. This study highlights the benefits of an integrated approach to outbreak investigations, involving the various elements of the PHLS and the Food Standards Agency, and acknowledges the full co-operation of the retailer in ensuring the rapid withdrawal of the contaminated product.

Disease Outbreaks↗

PubMed2000

RNA polymerase II in Cajal bodies of amphibian oocytes.

Cajal bodies (coiled bodies) are nuclear organelles that contain a variety of components required for transcription and processing of RNA. Cajal bodies in amphibian oocytes are stained by mAb H14, which recognizes the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II when the heptapeptide repeat is phosphorylated on serine-5. Oocytes were treated with the transcription inhibitor 5, 6-dichloro-1-beta-d-ribofuranosylbenzimidazole (DRB), which prevents phosphorylation of the CTD. Cajal bodies from oocytes that had been treated for 2-3 h with DRB no longer stained with mAb H14, but staining reappeared when the inhibitor was washed out. Epitope-tagged transcripts of two small subunits of polymerase II, RPB6 and RPB9, were injected into the cytoplasm of Xenopus and Triturus oocytes. Newly translated RPB6 and RPB9 were specifically targeted to Cajal bodies within 4 h, and Cajal bodies remained the site of highest concentration of tagged protein during the next 2 days. These data suggest that polymerase subunits pass through the Cajal bodies with a transit time no greater than a few hours. We discuss the possibility that Cajal bodies are sites of assembly or modification of the transcription machinery of the nucleus.

Animals↗

PubMed1991

'Outsourcing' will continue to gain clients.

It's an arrangement that puts the day-to-day operation of your information systems software in the hands of an outside manager at an outside computer system. The attraction of "outsourcing" is the money saved by not paying for hardware or in-house computer technicians. And it frees your top computer people for software improvement. But it's not for everyone. Owen Doyle lists the advantages and disadvantages.

Computer Communication Networks↗

PubMed1990

New info systems chiefs can use this as a primer.

You're newly in charge of information systems, or you've been in charge for a while but aren't comfortable yet with the complexity of technology and language. Owen Doyle has some tips to help you gain control and head in the right direction.

Hospital Administrators↗

PubMed1990

Making the most of information system consultants.

Choosing an information system consultant can be as critical as finding the right hardware and software. To increase efficiency and reduce wasted time, hospitals should evaluate the chain of events consultants traditionally follow in helping their clients select information systems. Hospitals should be aware of ways to streamline the process and should consider potential conflicts of interest that can arise if consulting firms have ties to vendors or system installers.

Commerce↗

PubMed1989