PubMed Health⌕ Search

Biomedical subjects

O Dubois

Publications and source records attributed to O Dubois.

At least 19 recordsLinked to original sources
PubMed2006

Developmental regulation of prostacyclin synthase and prostacyclin receptors in the ovine uterus and conceptus during the peri-implantation period.

This study documents the expression of prostacyclin (PGI2) synthase (PTGIS) and PGI2 receptors in the trophoblast and uterus of the ewe at the time of maternal recognition of pregnancy (i.e. days 7, 9, 12, 14 and 17). The membrane receptor for PGI2 (PTGIR) and the nuclear receptors, i.e. peroxisome proliferator-activated receptors (PPAR) and their heterodimer partners the retinoid X receptors (RXR), were analysed. In the endometrium, PTGIS transcript and protein were expressed at day 9 of pregnancy and levels declined from days 12 to 17. Immunohistochemistry and in situ hybridization indicated that PTGIS was mainly located in the luminal epithelium of the endometrium. Endometrial PTGIR, PPARA, PPARG and RXRG expression was regulated during the peri-implantation period whereas PPARD, RXRA and RXRB were consistently expressed. In the trophoblast, PTGIS transcript levels rose as development progressed and peaked at day 17. PTGIR and PPARA transcripts peaked before day 12 and then declined and became nearly undetectable by day 17, whereas PPARD and PPARG transcript levels rose steadily from days 12 to 17. Because the PPARs and the RXRs display different expression profiles, we suggest that different heterodimers may form and support distinct functions as development proceeds. Our results also underline the importance of PTGIS and PPARD in the trophoblast and PTGIR in the uterus, suggesting that PGI2 is of both uterine and trophoblastic origin and is involved in a complex signalling pathway at around the time of implantation in the ewe.

Animals↗
PubMed2004

Identification of differentially regulated genes during elongation and early implantation in the ovine trophoblast using complementary DNA array screening.

Following hatching, pre-elongated conceptuses undergo elongation by intense proliferation, until implantation. We investigated the changes in gene expression associated with these physiological events using human cDNA arrays containing 2370 known genes. Comparison of pre-elongated, elongated, and implanting trophoblasts allowed the determination of 313 expressed genes, 63 of which were differentially regulated. These were classified into four functional families. Pre-elongated trophoblasts were characterized by preferential expression of genes involved in protein trafficking, whereas only latter developmental stages expressed cell signaling genes and receptors. Among the 63 developmentally regulated genes, four exhibited the highest levels of expression (TMSB10, CTNNA1, NMP1, and CX3CL1). Each of these also represents a functional family and display a specific expression pattern. One of them, CX3CL1 (CX3C chemokine, also known as fractalkine), is a chemokine that seems to have potential importance in trophoblast development, and which deserves further clarification of its role in implantation.

Animals↗
PubMed2003

Rigorous decimation-based construction of ground pure states for spin-glass models on random lattices.

A constructive scheme for determining pure states at very low temperature in the 3-spins glass model on a random lattice is provided, in full agreement with Parisi's one step replica symmetry breaking (RSB) scheme. Proof is based on the analysis of a partial decimation procedure and of the statistical properties of its output, i.e., a reduced Hamiltonian acting on a subset of the initial spins. The number of ground states (GS) in each state, the number of states, and the distances between GS are calculated and correspond to RSB predictions.

Journal Article↗
PubMed1989

[Computerized obstetrical record of the North Pas-de-Calais region].

The computerized obstetrical record of the North-Pas-de-Calais region proceeds from the desire of a number of people from the Regional Health Observatory, to dispose of a prospective epidemiologic tool in the field of perinatology. The objective is a better knowledge of the cause of perinatal death, analyzing the modalities of prenatal monitoring, delivery conditions, transfer of the newborns, as well as socio-economical conditions. The program manages the computerization of a usual obstetrical file, in the region, including approximately 200 items and allowing every possible comparison with the well-known CCIM record. The program permits local treatment of the data (statistical calculations) in each maternity hospital: rapid transmission of information between maternity and correspondents (computerized personalized mail), a base of agreement between the different protagonists: midwives, pediatricians, specialists, an easier collaboration with the protection agencies and, especially, the Regional Direction of Sanitary and Social Affairs, with automatic response to hundreds of questions of the yearly questionnaire, which saves a great deal of time and an extreme reliability of the information. This program is compatible with any IBM PC microcomputer and includes a hard disk of 10 or 20 mega-octets. The current price (microcomputer with hard disk, monitor, keyboard, letter-quality printer, management program) make all these performances available at a relatively low expense, under 25,000 francs (not including the tax).

Female↗
PubMed1986

[Variations in birth weight as a function of various fetal and maternal characteristics. The application to the diagnosis of hypotrophy].

The known variations in weight for gestational age as well as compounding maternal and/or fetal factors, puts into question the usual definition of small for gestational age (SGA) which is based on gestational age alone. On the basis of more than 20,000 births studied in 4 maternity hospitals from 3 separate regions in France, the authors propose a new definition for growth retardation in full-term babies, taking into account 4 factors: gestational age, sex, birth rank, height and usual weight of mothers. This definition allows constitutionally SGA newborns to be considered normal, while some babies previously classified as normal would now lead one to suspect intra-uterine growth retardation (IUGR). This approach tries to take constitutional and environmental risk factors into account in fetal growth hopefully allowing for help better detection of IUGR.

Adult↗
PubMed1982

[Transfer of neonates in northern france. Factors of mortality (author's transl)].

An extensive study was undertaken in northern France from January 1st to May 31st 1978, concerning the transfer of neonates from maternity hospitals to specialized units. Analysis of 250 children whose birth weight was below 2,000 gm showed that several risk factors could be taken into account to reduce neonatal mortality in this area. Prematurity (22% children weighing less than 1,500 gm in those discharged alive, 54% in the dead), neonatal distress (36% versus 61% of resuscitation) hypothermia (7% versus 23% with temperature below 34 degrees 6 at the time of admission), the need for an other transfer (1% vs. 12%) seem to be features highly related with poor prognosis. This stresses the importance of the prevention of prematurity, of proper management of the babies in the maternity hospital, of the conditions of transport and of the choice of the neonatal unit.

France↗
PubMed1980

New concepts for a microprocessor oriented long term intelligent monitoring of newborns.

This paper is based on the utilization of the very elementary principle of linear regression used in a recursive way. This technique tested on electrophysiological signals readily leads to the conception of a monitoring system built on a biprocessor unit. In a clinical context, the use of microprocessors leads then to the design of very compact devices including the capability of distributed processing which embrances the concept of intelligent monitoring. Finally, a proposal is given for the realization of a complete monitoring control desk (MCD) devoted to the survey of eight patients.

Cardiography, Impedance↗
PubMed1980

Parallel evaluation of Astra 8 and Astra 4 multichannel analyzers in two hospital laboratories.

We evaluated the new Beckman multichannel analyzers Astra 8 and Astra 4. Both instruments performed the following tests: Na, K, Cl, CO2, urea, glucose, and creatinine in plasma and Na, K, Cl, and creatinine determinations in urine. We tested precision, accuracy, and linearity at the usual concentrations in plasma and urine. We compared the Astra 8 and Astra 4 with continuous-flow (SMA 6) and discrete analyzers such as IL 243, Beckman KLiNa Flame, Beckman System 1, Corning 920 M Chloride Meter, and the Corning Blood-Gas Analyzer 175. Special tests were performed on plasma-to-plasma and urine-to-plasma carryover. Both analyzers are easy to operate and suitable for both emergency and routine use.

Autoanalysis↗