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O F Lindroos

Publications and source records attributed to O F Lindroos.

11 recordsLinked to original sources
PubMed1993

Characterization of melatonin binding to the anteroventral and anterodorsal thalamic nuclei of the rat.

Binding of 2-[125I]melatonin to the anteroventral and anterodorsal nuclei of the rat limbic thalamus was analyzed with in vitro autoradiography. In both nuclei the binding was of high affinity (Kd values 24-41 pM) and was competed with by nanomolar concentrations of 6-hydroxymelatonin. Kd values were in the same range as those reported for other high-affinity binding sites in the brain. Bmax values were 0.68-2.99 fmol/mg protein. The results suggest that some effects of melatonin are mediated via the limbic thalamus.

Animals↗
PubMed1992

Melatonin binding to the anteroventral and anterodorsal thalamic nuclei in the rat.

Pineal melatonin is a putative humoral coordinator of various circadian body rhythms. Rather few loci in the brain contain such a density of melatonin receptors that specific binding can be demonstrated by autoradiography. In this study we measured, using in vitro receptor autoradiography, displaceable binding of iodo-melatonin to the anteroventral and anterodorsal thalamic nuclei of Wistar rats (0.6-1.4 fmol/mg protein at 100 pM ligand concentration). These nuclei of the limbic system have direct connections with the retina and they participate in learning and memory functions.

Animals↗
PubMed1991

Short Nissl staining for incubated cryostat sections of the brain.

Nissl stain often binds poorly to cryostat sections which have been incubated in solutions of radiolabeled ligands. Such incubation is used in receptor autoradiography of the brain when using the in vitro method. We have developed a rapid (16 min) modification of Nissl staining for sections that bind stain poorly, e.g., incubated sections. The method stains well sections which cannot be stained with other rapid Nissl staining methods.

Animals↗
PubMed1984

Overstimulation, occipital/somesthetic cerebral cortical depth, and cortical asymmetry in mice.

It was hypothesized on theoretical grounds that sensory multidimensional overstimulation (stress) during development might not yield increased cerebral cortical depth, as has been found for enriched rearing conditions. We found that such overstimuliation in growing mice resulted in a low occipital/somesthetic cortical depth ratio in comparison to EC-stimulated mice (EC, enriched conditions). It is unclear whether this is due to small occipital or large somesthetic cortical depth. Principal-component analytic techniques indicate a complicated interaction during development involving sex, environmental condition, and cortical asymmetry.

Animals↗
PubMedDate not supplied

A cheap, calibrated photographic method for the screening of X-ray film autoradiographs.

This report describes the use of a calibrated photographic method for the screening of X-ray film autoradiographs. In the method the autoradiographic film is contact printed onto photographic film of high contrast, and the latter is contact printed onto photographic paper of suitable contrast. Special features of e.g. deoxyglucose-autoradiographs are thus visualized. The method is inexpensive, and it can be used as a complement to densitometric point measurements of X-ray film autoradiographs.

Animals↗