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Biomedical subjects

O F Scheiffarth

Publications and source records attributed to O F Scheiffarth.

At least 19 recordsLinked to original sources
PubMed1993

Clinical and immunohistochemical correlation of herpetic keratitis with the expression of HLA-DR antigen.

The corneal buttons after corneal graft of 22 patients with herpetic keratitis were studied (16 male, 6 female; 7-87 years of age). Ten still had active inflammation, while 12 had stabilized by the time of the study. Different degrees of neovascularization were observed in 19 cases. HLA-DR antigen was detected in the corneal epithelium in 7 of 22 cases (32%), in the corneal stroma in 17 of 22 cases (77%), and in the endothelium in 9 cases (41%) by using the immunohistochemical technique. The expression of HLA-DR antigen was more common and more marked in active herpetic keratitis (90%) and in neovascularized corneas (84%). The frequency and density of HLA-DR expression in perforating corneal ulcers were no higher than in other cases, but anterior synechia was accompanied by strong expression of HLA-DR antigen in the corneas. The expression of HLA-DR antigen was not only detected in the peripheral, but also in the central area of the specimens. The cells expressing HLA-DR antigen in corneas were mainly corneal cells. The results showed a close relationship between HSV-keratitis and the expression of HLA-DR antigen, which may be induced during the clinical course of the disease. The expression of HLA-DR might be one of the factors in the recurrent onset of the disease and a significant sign of the prognosis of corneal transplantation for these patients.

Adolescent↗
PubMed1993

Cells of the immune system and their cytokines in epiretinal membranes and in the vitreous of patients with proliferative diabetic retinopathy.

In the present study, we specifically investigated the presence of the main immune cells, namely T helper/inducer lymphocytes, T suppressor/cytotoxic lymphocytes, B lymphocytes and macrophages, for HLA-DR antigen expression and the cytokines IL-1 alpha and IL-2 in epiretinal membranes of proliferative diabetic retinopathy (PDR) using immunohistochemical staining. The levels of the two cytokines (IL-1 alpha and IL-2) in the vitreous were measured by ELISA. The results show that 18 out of the 32 epiretinal specimens reacted positively with monoclonal anti-CD4 (marker for T helper/inducer cell subset) antibody (56%), 21 reacted with monoclonal anti-CD8 (marker for T suppressor/cytotoxic subset) antibody (66%), 15 reacted with monoclonal anti-CD22 (marker for B lymphocytes) antibody (47%), and 25 (78%) were positive for monoclonal anti-macrophage and anti-HLA-DR antibodies. IL-1 alpha and IL-2 were detected in the epiretinal membranes in 8 out of 13 tested cases (62%). However, in the vitreous, IL-1 alpha was detected in only 3 out of 13 cases (70, 75 and 80 pg/ml, respectively), while IL-2 was not detected in any of the vitreous samples. The results clearly demonstrate that immune cells and their cytokines were found in about half of the epiretinal membrane specimens. The invasion of the immunocompetent cells in the membranes seems to be a secondary event. They would not participate in the pathogenesis of PDR, but probably induce unspecific reactions and thus complicate the disease.

Antigens, CD↗
PubMed1992

Immunoimaging of choroidal melanoma: assessment of its diagnostic accuracy and limitations in 101 cases.

Immunoscintigraphy (IS) was performed on 101 patients with space occupying intraocular lesions including choroidal melanomas (85), choroidal naevi (11), non-melanoma metastases (three), and other melanoma simulating lesions (two). Scintigraphic images with conventional and emission computer tomography techniques were obtained after the intravenous injection of 99mTc-labelled F(ab')2 fragments of monoclonal antibody (MoAb) 225.28S directed against the high molecular weight-melanoma associated antigen (HMW-MAA). Immunohistochemistry was performed on sections of four out of 10 melanoma-containing eyes to confirm MoAb binding. IS demonstrated positive scans in 66 out of 85 choroidal melanomas, offering a sensitivity of 78%. Sensitivity was dependent on the lesion size. True negative results were obtained in 15 out of 16 non-melanoma lesions (specificity 94%). False positive antibody accumulation was found in one patient with a post-traumatic subretinal haemorrhage. Immunohistochemistry demonstrated positive MoAb 225.28S binding in all melanoma sections. In summary IS offered substantial sensitivity and specificity in the differentiation of intraocular lesions, particularly choroidal melanomas, naevi, and metastases. In combination with other diagnostic procedures such as ultrasound echography and fluorescein angiography IS proved to be a valuable method in the diagnosis of choroidal melanoma.

Choroid↗
PubMed1992

Lymphocytes, macrophages and HLA-DR expression in vitreal and epiretinal membranes of proliferative vitreoretinopathy. An immunohistochemical study.

Proliferative vitreoretinopathy (PVR) as a severe complication of retinal detachment and also the main cause of failure in its treatments is not yet completely understood. In the present study, we examined 16 PVR epiretinal membrane specimens removed during vitrectomy from 16 patients with complicated rhegmatogenous retinal detachment by immunostaining of cryosections. We looked for the presence of lymphocytes and detected helper/inducer T lymphocytes (CD4+), suppressor/cytotoxic T lymphocytes (CD8+), B lymphocytes (CD22+), macrophages (CD68+), and HLA-DR expression. The results showed that 6 out of 16 specimens reacted positively with monoclonal antibody against the helper/inducer T cell subset (38%), 5 out of 16 reacted positively with a suppressor/cytotoxic T cell-specific monoclonal antibody (31%), 7 out of 16 reacted with B cell-specific monoclonal antibody (44%), and 14 out of 16 were positive for macrophages and HLA-DR (88%). Nevertheless, we think that the invasion of macrophages and lymphocytes into the membranes is not the cause for the pathogenesis, but a secondary event that might further complicate the course of the disease.

Antibodies, Monoclonal↗
PubMed1990

[Allergies of eyelids and eyes].

Immunopathological hypersensitivity reactions frequently involve the eye and its adnexa. Owning to their exposed position in the body, the eyelids and eyes are affected by a variety of allergic reactions. Autoimmune diseases often involve the highly vascular uvea, but also the conjunctiva, cornea and retina.

Combined Modality Therapy↗
PubMed1990

[AIDS and the eye].

Seventy percent of patients with AIDS have AIDS-related ocular involvement. This is a special challenge for the ophthalmologist which requires specific knowledge. The nature and frequency of the ocular involvement as well as the time of manifestation during the HIV-disease are discussed. The potential therapy for such opportunistic infections is discussed. Furthermore, the HIV-specific aspects for disinfection procedures in the office (tonometry, three-mirror lens, contact lenses) are also elucidated.

Acquired Immunodeficiency Syndrome↗
PubMed1990

[An immunohistochemical study of vitreal and epiretinal membranes in human eyes].

Macrophages, monocytes and the expression of HLA-DR antigen in 52 cases of various vitreal and epiretinal membranes in human eyes were studied with the immunohistochemical technique. The results showed that more than 80% of the specimens were macrophage positive and expressed HLA-DR antigen, and monocytes were mainly seen in the specimens of proliferative diabetic retinopathy (41.7%). Macrophages were considered to play an important role in the development of epiretinal membranes, and immuno-response was also one of the related factors.

Antibodies, Monoclonal↗
PubMed1990

Intraocular in vivo immunofluorescence. A new technique for visualizing structures of the ocular fundus.

A new noninvasive technique for observing structures in the living eye has been developed: the technique is based on intravenous application of specific antibodies, labeled with fluorescein. The immunologically marked substructures of the fundus of the eye, the only transparent organ in mammals, are photographed directly using high speed film. Combined with a digital image processor to enhance low contrast contours, this technique can be used for diagnostic purposes, as shown in rabbits with experimental toxoplasmic chorioretinitis.

Animals↗
PubMed1990

[Macrophages and HLA-DR expression in proliferative vitreoretinopathy].

Macrophages and macrophage-like cells have been reported as cellular components of epiretinal and vitreous membranes. The origin and function of these versatile cells in the membranes are not fully understood. In the present study we used monoclonal antibodies reacting with macrophages, HLA-DR and monocytes (OKM5) to investigate cells in 52 specimens of various human epiretinal and vitreous membranes collected by vitrectomy. The results show that more than 80% of all membranes are positive for macrophage and HLA-DR antigen. HLA-DR is considered to be expressed by the macrophages. Cells reacting with antimonocyte (OKM5) are seen sporadically only in proliferative diabetic retinopathy. These results are discussed with respect to the possible origin of the macrophages and their important role in the development and formation of epiretinal and vitreal membranes.

Antibodies, Monoclonal↗
PubMed1989

Expression of HLA-DR antigen in epiretinal and vitreous membranes.

The pathogenic mechanisms of epiretinal membranes are not clearly understood nowadays in ophthalmology. Trying to elucidate it from another aspect, we examined the presence of HLA-DR antigen in 41 epiretinal membrane specimens from patients with proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR) by using immunohistochemical technique (APAAP). The results showed that 82.93% of the membranes (34 out of 41) were HLA-DR antigen positive. HLA-DR antigen was considered to be expressed by macrophages in epiretinal membranes. The findings here reveal that the formation and development of epiretinal membranes are probably correlated with immune responses.

Diabetic Retinopathy↗
PubMed1989

[New immune proteins and their perspectives for ophthalmology].

Research and progress in immunology and microbiology made the characterization and production of immunological active proteins possible. Lymphokines and monoclonal antibodies can be synthesized by new methods of gene technology and cell fusion techniques. These proteins meanwhile are of essential importance in biomedical research and are becoming an important tool in clinical medicine too. The possibility to modify the proteins or to label them with cytotoxic substances provides exciting aspects of medical progress for the future. Applications in ophthalmology hopefully will lead to new and successful ways for the diagnosis and therapy of ocular diseases.

Antibodies, Monoclonal↗
PubMed1989

[Tumor markers in malignant choroid melanoma].

Antibodies against tumor-associated antigens ("tumor markers") can be used for the diagnosis of malignant tumors. Eight different monoclonal antibodies, that can recognize tumor-associated antigens of dermal melanomas, were tested in cryostat sections of uveal melanomas. The uveal melanomas showed antigenic differences (e.g., gangliosides) and common antigens (e.g. high molecular weight melanoma-associated antigen) in comparison to dermal melanomas. The implications of these results for diagnosing and even treating uveal melanomas are discussed.

Alkaline Phosphatase↗
PubMed1989

[The effect of Co-60 irradiation on the monomer fraction of intraocular PMMA lenses].

Sterile inflammatory reactions of varying degree are a rare, but not uncommon complication after intraocular lens implantation. At present we still do not know the cause of these processes, but we consider two chemical substances at least as risk factors for them: the lens content of monomeric methyl methacrylate, which is present in any lens made from polymethyl methacrylate (Perspex), and the lens content of ethylene oxide, an extremely toxic agent, which is absorbed by the lens during sterilization with this gas. Gaschromatographic investigations show that under sterilization of intraocular lenses by Co60-radiation the lens content of monomeric methylmethacrylate can be reduced drastically. Also, any contamination of the lens by ethylene oxide is avoided. Therefore at least two important arguments speak in favour of this alternative sterilization technique.

Chromatography, Gas↗
PubMed1988

Proteins of the extracellular matrix in vitreoretinal membranes.

Epiretinal and vitreous membranes of different etiology, e.g., in diabetic retinopathy, following retinal detachment, trauma or inflammatory processes, show a similar morphology. The exact composition of the extracellular matrix and the pathogenesis of these membranes remain uncertain. The presence of collagens, type I-IV, laminin, and fibronectin can be shown by means of immunofluorescence with affinity-purified antibodies. Collagen type V was revealed by SDS-polyacrylamide-gel electrophoresis. These proteins of the extracellular matrix have diverse properties and functions in the membranes, as is discussed. Despite great similarities in morphology, there are some differences in the matrix, seemingly dependent upon the etiology of the membrane.

Collagen↗
PubMed1988

Immunoscintigraphy in intraocular malignant melanoma.

Immunoscintigraphy of malignant tumours has become an encouraging tool in nuclear medicine. Early diagnosis of small lesions is mandatory for successful cancer therapy. The scintigraphic detectability of small lesions (less than 1 cm in diameter) by immunoscintigraphy is shown in 39 patients suffering from ocular tumours (37 malignant choroidal melanoma, two benign choroidal naevi) using 99Tcm-labelled F(ab')2 fragments of the anti-melanoma monoclonal antibody 225.28S; this antibody recognizes the high molecular weight melanoma-associated antigen. No adverse effects were observed. In terms of true-positive results, single photon emission computed tomography (SPECT) proved to be superior to planar scans (73% versus 41% true-positive results).

Aged↗
PubMed1988

Reducing the amount of monomers in intraocular lenses through sterilization by gamma radiation.

Sterile inflammatory reactions of varying degrees are a rare, but not uncommon, complication after intraocular lens implantation. At present we still do not know the cause of these processes, but we consider two chemical substances contained in the lens at least as risk factors for them: monomeric methyl methacrylate, which is present in any lens made from polymethyl methacrylate (Perspex), and ethylene oxide, an extremely toxic agent, which is absorbed by the lens during sterilization with this gas. Gas chromatographic investigations show that under sterilization of intraocular lenses by 60Co radiation the amount of monomeric methyl methacrylate in the lens can be reduced drastically. Also, any contamination of the lens by ethylene oxide is avoided. Therefore, at least two important arguments speak in favor of this alternative sterilization technique.

Chromatography, Gas↗