[Immuno-allergic thrombopenia caused by proxibarbal].
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Adult↗
Biomedical subjects
O Fardel
Publications and source records attributed to O Fardel.
Differentiation of the P-gp and MRP1 multidrug resistance systems by mobile lipid 1H-NMR spectroscopy and phosphatidylserine externalization.
We have previously demonstrated that proton NMR spectra of fatty acid chains in erythroleukemia K562 wild-type cells and their MDR1 counterparts show variations related to the phenotype over-expressing the P-glycoprotein (P-gp). Human lung cancer cells whose multidrug resistance (MDR) counterparts over-express the multidrug resistance-associated protein MRP1 have not yet been studied by NMR. Both P-gp and MRP1 belong to the same ATP-binding cassette transporter superfamily. A comparison of NMR spectra from both these multidrug-resistance phenotypes showed that the results previously obtained on the MDR1 family are not valid for MRP1. Furthermore, flow cytofluorimetry studies with external phosphatidylserine labelling showed that P-gp and MRP1 overexpressions have strong but differentiated effects on cell lipid pools.
ATP Binding Cassette Transporter, Subfamily B, Mem↗
[Flow cytometry: application for the diagnosis and the follow-up of hematological malignancies].
Diagnosis of haematological malignancies is based on multiparametric analysis such as morphology, phenotype and genotype studies. Some entities are only defined by one of these approach. Flow-cytometry (FCM) is useful to determined the normal counterpart of the tumoral process and its differentiation status within the involved lineage. Furthermore, FCM is able to detect clonality in B or T proliferations and criteria for malignancies such as abnormal phenotype. Finally it also specifies prognosis criterias. Among the different haematological malignancies, acute lymphoblastic leukaemia (ALL) can be diagnosed using FCM, whereas acute myeloblastic leukaemia diagnosis is only confirmed by this methodology, which could moreover determine prognosis factors. A scoring system (EGIL) determine the normal counterpart of tumoral cells using a panel of different markers. Immunophenotyping is also useful in chronic lymphoproliferative disorders, such as chronic lymphocytic leukaemia (CLL) by using a similar scoring system (so-called Matutes scoring). Since FCM is able to detect simultaneously numerous cell markers it could be more accurate than immunohistochemistry for the diagnosis of follicular lymphoma, mantle cell lymphoma or hairy cell leukaemia. Finally, during treatment follow-up, minimal residual disease characterised by the detection of rare specific events, may be examined using FCM, in some situations.
Antibodies, Monoclonal↗
Conformational changes in membrane proteins of multidrug-resistant K562 and primary rat hepatocyte cultures as studied by Fourier transform infrared spectroscopy.
The multidrug resistance (MDR) phenotype has been investigated by means of Fourier transform infrared spectroscopy (FT-IR/S) on cell smears. We investigated K562 cell lines (sensitive and doxorubicin-resistant, the latter being MDR too) and primary cultures of rat hepatocytes (HEP). HEP displayed elevated levels of P-glycoprotein (P-gp) with time in 2-4 day-old culture, thus developing in the same time a MDR phenotype. No functional P-gp activity could be detected in HEP at day 1 after cell seeding. Given the sensitivity of FT-IR/S and using computational treatment of FT-IR data, we found that spectra of MDR-K562 and HEP from day 2 to day 4 displayed close protein conformational changes involving beta-sheets. These changes might be in close relationship with the MDR-phenotype and P-gp overexpression.
ATP Binding Cassette Transporter, Subfamily B, Mem↗