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Biomedical subjects

O G Zanen-Lim

Publications and source records attributed to O G Zanen-Lim.

10 recordsLinked to original sources
PubMed1988

Differences in lipopolysaccharide profiles of serologically identical Legionella pneumophila serogroup 6 strains.

Over five years 18 strains of Legionella pneumophila serogroup 6 were isolated in Amsterdam from the hot water supply in three hospitals and from one patient. Immunodiffusion and immunoblot procedures showed that these strains were identical. Profiles of isolated lipopolysaccharides from the 18 strains and the reference serogroup 6 strain were visualised in polyacrylamide gels stained with silver. Four strains from hospital A, isolated in 1982, 1984, and 1985 displayed similar lipopolysaccharide profiles which were different in relative mobility from those of hospitals B and C. Those from hospital B (12 strains isolated in 1983 and 1986) and C (one strain) were similar in relative mobility but different in colour. The strain from a patient with acquired immune deficiency syndrome (AIDS) in hospital A displayed a lipopolysaccharide profile characteristic of hospital A. These reproducible profiles were all different in relative mobility from the reference serogroup 6 strain. They can be used as a marker system in epidemiological surveys of serologically identical serogroup 6 strains. Lipopolysaccharide patterns from strains isolated throughout the years in the same hospital were similar. This suggests an outgrowth from organisms inhabiting the plumbing system rather than reseeding from the Amsterdam mains supply.

Electrophoresis, Polyacrylamide Gel↗
PubMed1984

Comparison of strains of Legionella pneumophila serogroup 1 isolated in four Amsterdam hospitals from patients and hot-water supplies.

Legionella pneumophila serogroup 1 was isolated from patients (14 strains) and hot-water taps (49 strains) in four hospitals in Amsterdam. Precipitation patterns obtained by reaction of heated cell suspensions and rabbit antisera in the double diffusion test revealed antigenic properties of serogroup 1 strains from water unique to each of the four hospitals. By this method similarity could be demonstrated between strains from patients and hot water in three of the four hospitals. No such relationship was present in the fourth hospital, a fact that could be explained by the case histories of the patients. Comparison of strain antigens in the double diffusion test is simple and suitable for epidemiological studies of L. pneumophila.

Antigens, Bacterial↗
PubMed1980

Postmortem bacteriology of the lung by printculture of frozen tissue. A technique for in situ culture of microorganisms in whole frozen organs.

Lung lobes obtained at necropsy from 100 patients were frozen at -70 degrees C and sectioned as a whole in a sledge microtome as used on complete frozen animals. Prints of freshly cut surfaces were cultured on different solid media in 14 cm Petri dishes. The printcultures showed three patterns of bacterial growth: dense growth in 24%, patchy growth in 43%, and no significant growth in 33%. Forty-two of the 67 positive printcultures showed to or more bacterial species. Besides bacteria known to be pathogens of the lung, streptococci of the viridans group, enterococci, and streptococci of group B were grown, sometimes in pure cultures. The patterns of the printcultures were reproducible in successive sections, and the quantity of the bacterial species could be assessed. Contaminating bacteria on the surface of the lung lobe could be recognised, as these produced colonies restricted to the edges of the print. Because there was no smearing of infecting and contaminating bacteria, printculture offers a method of reference.

Adult↗
PubMed1977

[Hepatitis A].

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Animals↗
PubMed1976

Virus-like particles demonstrated by freeze-squeeze technique in acute-phase serum of patients with HBAg-negative hepatitis.

Electromicroscopy of fluid, squeezed from excised and frozen precipitation lines obtained by immunoelectrophoresis in agarose gels of acute-phase sera from patients with HBAg-negative hepatitis, revealed particles of 22, 27, and 31-41 nm. These precipitates and particles were not present in normal human sera and disappeared from the sera of the patients after recovery. These particles were still demonstrable after two years' storage at -20 degrees C.

Adult↗