Biomedical subjects

O Güntürkün

Publications and source records attributed to O Güntürkün.

At least 19 recordsLinked to original sources

PubMed2006

Organization of telencephalotectal projections in pigeons: Impact for lateralized top-down control.

Birds display hemispheric specific modes of visual processing with a dominance of the right eye/left hemisphere for detailed visual object analysis. In pigeons, this behavioral lateralization is accompanied by morphological left-right differences in the ascending tectofugal pathway. This system is also asymmetrically modulated by descending telencephalotectal input whereby the left forebrain displays a much more pronounced physiological control over ipsilateral left and contralateral right visual thalamic processes. In the present study we aimed to answer the question if this top-down asymmetry that up to now had been demonstrated in single cell recording studies is due to anatomical asymmetries in the size of the fiber systems descending from the telencephalon to the tectum. We approached this question by means of a quantitative retrograde tracing study. Cholera toxin subunit B (CtB) was injected unilaterally into either the left or right optic tectum of adult pigeons. After immunohistochemical detection of CtB-positive cells, the number of ipsi- and contralaterally projecting neurons was estimated. Retrogradely labeled cells were located within the arcopallium, the hyperpallium apicale (HA) and the temporo-parieto-occipital area (TPO). Descending projections from HA, arcopallium, and TPO were mainly or exclusively ipsilateral with the contralateral projection being extremely small. Moreover, there was no difference between left and right hemispheric projections. These anatomical data sharply contrast with behavioral and electrophysiological ones which reveal an asymmetric and bilateral top down control. Therefore, contralateral and lateralized forebrain influences onto tectofugal processing are possibly not the direct result of asymmetrical descending axon numbers. Those influences emerge by a lateralized intra- and/or interhemispheric integration of ascending and descending input onto the rotundus.

Animals↗

PubMed2006

Lateralized cognition: asymmetrical and complementary strategies of pigeons during discrimination of the "human concept".

This study was aimed at revealing which cognitive processes are lateralized in visual categorizations of "humans" by pigeons. To this end, pigeons were trained to categorize pictures of humans and then tested binocularly or monocularly (left or right eye) on the learned categorization and for transfer to novel exemplars (Experiment 1). Subsequent tests examined whether they relied on memorized features or on a conceptual strategy, using stimuli composed of new combinations of familiar and novel humans and backgrounds (Experiment 2), whether the hemispheres processed global or local information, using pictures with different levels of scrambling (Experiment 3), and whether they attended to configuration, using distorted human figures (Experiment 4). The results suggest that the left hemisphere employs a category strategy and concentrates on local features, while the right hemisphere uses an exemplar strategy and relies on configuration. These cognitive dichotomies of the cerebral hemispheres are largely shared by humans, suggesting that lateralized cognitive systems already defined the neural architecture of the common ancestor of birds and mammals.

Animals↗

PubMed2005

Transcallosal inhibition across the menstrual cycle: a TMS study.

OBJECTIVE: To determine if there are steroid-dependent changes in transcallosal transfer during the menstrual cycle in normal women. METHODS: We tested 13 normally cycling women during the menstrual, follicular and midluteal phases. Blood levels of estradiol (E) and progesterone (P) were determined by radioimmunoassay. Ipsilateral tonic voluntary muscle activity suppression, called ipsilateral silent period (iSP), was evoked by applying transcranial magnetic stimulation (TMS) over the left motor cortex and by measuring the EMG of the ipsilateral first dorsal interosseus (FDI) muscle. Both iSP-duration and transcallosal conduction times were measured and related to cycle phase and steroid levels. RESULTS: Duration of iSPs varied over the cycle with largest differences between follicular and midluteal phases. During the midluteal phase high levels of P were significantly related to short iSPs. This relation also applied to E levels and iSPs during the follicular phase. CONCLUSIONS: Our study shows for the first time that the transcallosal transfer is modulated by E and P and changes over the menstrual cycle. SIGNIFICANCE: It is suggested that gonadal steroid hormones affect the interhemispheric interaction and change the functional cerebral organization sex dependently via its neuromodulatory properties on GABAergic and glutamatergic neurons.

Adult↗

PubMed2005

Neuron numbers in sensory cortices of five delphinids compared to a physeterid, the pygmy sperm whale.

With its large mass and enormous gyrification, the neocortex of whales and dolphins has always been a challenge to neurobiologists. Here we analyse the relationship between neuron number per cortical unit in three different sensory areas and brain mass in six different toothed whale species, five delphinids and one physeterid. Cortex samples, including primary cortical areas of the auditory, visual, and somatosensory systems were taken from both hemispheres of brains fixed in 10% buffered formalin. The samples were embedded in paraffin, sectioned at 25 microm thickness and stained with cresyl violet. Because cortical thickness varies among toothed whale species, cell counts were done in cortical units measuring 150mum in width, 25 microm in thickness, and extending from the pial surface to the white matter. By arranging the delphinid brains according to their total mass, 834-6052 g, we found decreasing neuron numbers in the investigated areas with increasing brain mass. The pigmy sperm whale (Kogia breviceps), a physeterid with an adult brain weight of 1000 g had a distinctly lower neuron number per cortical unit. As had been expected, an increase in adult brain weight in delphinid cetaceans (family Delphinidae) is not correlated with an increase in neuron number per cortical unit.

Animals↗

PubMed2005

Right ear advantage for conspecific calls in adults and subadults, but not infants, California sea lions (Zalophus californianus): hemispheric specialization for communication?

This paper explores functional hemispheric asymmetries in the perception of auditory signals in a marine mammal species, the sea lion. Using a head-orienting task toward sounds we found a right ear--left hemisphere--advantage for conspecific calls in adult and subadult California sea lions (Zalophus californianus) that was absent in infants. Non-conspecific sounds did not elicit lateralized reactions in any age group. These findings show that maturational steps regarding communication in the brain of pinnipeds are similar to those described in primates. Such a result in a semi-aquatic species distant from primates on the phylogenetic tree speaks for a stability and an ancient emergence of the left hemispheric specialization for vocal communication. The origin of what seems to be a widespread brain feature might be searched in the temporal and spectral communicative sound's characteristics rather than in its semantic value.

Age Factors↗

PubMed2004

Post-hatch activity-dependent modulation of visual asymmetry formation in pigeons.

The embryonically induced visual lateralization in pigeons can be modified by occlusion of one eye after hatching. Here we show that this deprivation effect could be also attained by short-term blocking of retinal activity with tetrodotoxin (TTX), leading to a dominance of the ipsilateral hemisphere in a visual discrimination task. This lateralization pattern resulted from a performance increase conveyed by the non-deprived hemisphere, while performance with the TTX-injected eye did not differ from that of saline-injected controls. Thus, post-hatch modulation of visual lateralization is mediated by TTX-sensitive, activity-dependent neuronal mechanisms. The transient silencing of one visual input alters the activity balance between the left and right eye system, enhancing visuoperceptive skills in the relatively higher active hemisphere.

Animals↗

PubMed2003

Catecholaminergic and dopamine-containing neurons in the spinal cord of pigeons: an immunohistochemical study.

Within the different species belonging to the vertebrate radiation, catecholaminergic elements of the spinal cord present a partly conservative, partly variable pattern. Unfortunately, the overall picture is far from clear since the situation for birds is largely obscure. Therefore, we examined the distribution of dopamine (DA)- and tyrosine hydroxylase (TH)-positive cells and fibers in the spinal cord of the adult pigeon by immunohistochemistry. TH-immunoreactive cells were located within two restricted areas. One group of cells with multipolar shape was located in laminae VI and VII, close to the white-gray border. These cells were more frequently found at rostral and caudal levels while being scarce at cervical-thoracic levels. The second group of cells was located in lamina VIII surrounding the central canal. These cells were bipolar in shape and were found ventrally and laterally to the central canal, with most of them contacting the lumen of the canal through a separate process. The TH-immunoreactive fibers were distributed in both the gray and the white matter. In the gray matter, they were mainly distributed around the central canal (lamina VIII), in the ventral horn close to the border of laminae VII-IX and in the lateral part of the dorsal horn in laminae II-VI. In the white matter the fibers were present in the lateral columns running longitudinal to the main axis. DA-immunoreactive cells were also located within two restricted areas, closely matching the distribution of TH-immunopositive ones. Additionally, the DA-immunoreactive cells had the same shape as the TH-immunoreactive cells, as bipolar neurons contacted the central canal and multipolar ones were located in the laminae VI and VII. Also the distribution of DA- and TH-immunoreactive fibers roughly matched. Both, DA-immunoreactive cells and fibers were scarcer than TH-immunoreactive ones. This finding suggests that the catecholaminergic system in the spinal cord consists of DA-immunoreactive cells as well as other catecholaminergic cells.

Animals↗

PubMed2002

Electrophysiological and morphological properties of cell types in the chick neostriatum caudolaterale.

The neostriatum caudolaterale, in the chick also referred to as dorsocaudal neostriatal complex, is a polymodal associative area in the forebrain of birds that is involved in sensorimotor integration and memory processes. We have used whole-cell patch-clamp recordings in chick brain slices to characterize the principal cell types of the neostriatum caudolaterale. Electrophysiological properties distinguished four classes of neurons. The morphological characteristics of these classes were examined by intracellular injection of Lucifer Yellow. Type I neurons characteristically fired a brief burst of action potentials. Morphologically, type I neurons had large somata and thick dendrites with many spines. Type II neurons were characterized by a repetitive firing pattern with conspicuous frequency adaptation. Type II neurons also had large somata and thick dendrites with many spines. There was no clear morphological distinction between type I and type II neurons. Type III neurons showed high-frequency firing with little accommodation and a prominent time-dependent inward rectification. They had thin, sparsely spiny dendrites and extensive local axonal arborizations. Electrophysiological and morphological properties indicated them as being interneurons. Type IV neurons had a longer action potential duration, a larger input resistance, and a longer membrane time constant than the other classes. Type IV neurons had small somata and short dendrites with few spines. The long axon collaterals of neurons in all spiny cell classes (types I, II, IV) followed similar patterns, suggesting that neurons from all these types can contribute to the projections of the neostriatum caudolaterale to sensory, limbic and motor areas. The electrophysiological and anatomical characterization of the major classes of neurons in the caudal forebrain of the chick provides a framework for the investigation of sensorimotor integration and learning at the cellular level in birds.

Action Potentials↗

PubMed2002

Impaired learning of a color reversal task after NMDA receptor blockade in the pigeon (Columba livia) associative forebrain (neostriatum caudolaterale).

The neostriatum caudolaterale (NCL) in the pigeon (Columba livia) forebrain is a multisensory associative area and a functional equivalent to the mammalian prefrontal cortex (PFC). To investigate the role of N-methyl-D-aspartate (NMDA) receptors in the NCL for learning flexibility, the authors trained pigeons in a color reversal task while locally blocking NMDA receptors with D,L-2-2-amino-5-phosphonovalerate (AP-5). Controls received saline injections. AP-5-treated pigeons made significantly more errors and showed significantly stronger perseveration in a learning strategy applied by both groups but were unimpaired in initial learning. Results indicate that NMDA receptors in the NCL are necessary for efficient performance in this PFC-sensitive task, and that they are involved in extinction of obsolete information rather than in acquiring new information.

Animals↗

PubMed2001

Nucleus isthmi, pars semilunaris as a key component of the tectofugal visual system in pigeons.

The avian isthmic nuclei are constituted by a group of structures reciprocally connected with the tectum opticum and considered to play a role in the modulation of intratectal processes. Although the two larger isthmic nuclei, the n. isthmi pars parvocellularis (Ipc) and the n. isthmi pars magnocellularis (Imc), have been studied in detail previously, the third and smallest of this group, the n. isthmi pars semilunaris (SLu), has been largely neglected. The present study demonstrates this isthmic component to be characterized by a unique connectivity and immunohistochemical pattern: 1) SLu receives tectal afferents and projects back onto the outer retinorecipient tectal layers; 2) it projects bilaterally onto the nucleus rotundus and thus modulates the ascending tectofugal system; 3) in addition, previous studies have demonstrated SLu projections onto the lateral spiriform nucleus (SpL), which mediates basal ganglia output onto the tectum. In that SpL projects onto the deep layers of the tectum, SLu indirectly modulates descending tectal output patterns. Taken together, the role of SLu goes far beyond a local modulation of intratectal processes. Instead, this isthmic structure is likely to play a key role in the topographically organized modulation of the ascending and, at least indirectly, also the descending projections of the optic tectum.

Animals↗

PubMed2001

Electrophysiological and anatomical evidence for a direct projection from the nucleus of the basal optic root to the nucleus rotundus in pigeons.

A direct projection of the nucleus of the basal optic root (nBOR) onto the nucleus rotundus (Rt) in the pigeon would link the accessory optic system to the ascending tectofugal pathway and could thus combine self- and object-motion processes. In this study, injections of retrograde tracers into the Rt revealed some cells in central nBOR to project onto the ipsilateral Rt. Contrary, injections into the diencephalic component of the ascending thalamofugal pathway resulted in massive labeling of neurons in dorsal nBOR. Single unit recordings showed that visual nBOR units could be activated by antidromic stimulation through the Rt. Successful collision tests applied to nBOR cells revealed that the connection between nBOR and Rt is direct. These data provide strong evidence for a direct and differential projection of nBOR subcomponents onto the thalamic relays of the two ascending visual pathways.

Animals↗

PubMed2001

Structural organization of parallel information processing within the tectofugal visual system of the pigeon.

Visual information processing within the ascending tectofugal pathway to the forebrain undergoes essential rearrangements between the mesencephalic tectum opticum and the diencephalic nucleus rotundus of birds. The outer tectal layers constitute a two-dimensional map of the visual surrounding, whereas nucleus rotundus is characterized by functional domains in which different visual features such as movement, color, or luminance are processed in parallel. Morphologic correlates of this reorganization were investigated by means of focal injections of the neuronal tracer choleratoxin subunit B into different regions of the nuclei rotundus and triangularis of the pigeon. Dependent on the thalamic injection site, variations in the retrograde labeling pattern of ascending tectal efferents were observed. All rotundal projecting neurons were located within the deep tectal layer 13. Five different cell populations were distinguished that could be differentiated according to their dendritic ramifications within different retinorecipient laminae and their axons projecting to different subcomponents of the nucleus rotundus. Because retinorecipient tectal layers differ in their input from distinct classes of retinal ganglion cells, each tectorotundal cell type probably processes different aspects of the visual surrounding. Therefore, the differential input/output connections of the five tectorotundal cell groups might constitute the structural basis for spatially segregated parallel information processing of different stimulus aspects within the tectofugal visual system. Because two of five rotundal projecting cell groups additionally exhibited quantitative shifts along the dorsoventral extension of the tectum, data also indicate visual field-dependent alterations in information processing for particular visual features.

Animals↗

PubMed2001

Distribution of BDNF, NT-3, trkB and trkC in the developing retino-tectal system of the pigeon (Columba livia).

The distribution of the neurotrophins BDNF and NT-3 as well as their corresponding high-affinity receptors trkB and trkC was characterized by immunohistochemistry in the developing retino-tectal system of the pigeon. These neurotrophins are known to be important for survival and development of neuronal tissues, but also for activity-dependent neuronal plasticity. In pigeons visual asymmetry is established at the morphological and behavioral level due to a natural asymmetrical light input before hatch, which is followed by a posthatch period of consolidation with unbiased light stimulation. Since the retino-tectal system is the crucial entity of these events, we studied the retinal and the tectal distribution of these neurotrophins and their receptors during retino-tectal formation, to analyze the developmental sequences to which these neurotrophins are tuned. Here we demonstrate that in altricial pigeons no retinal immunolabeling of BDNF, NT-3 or their receptors could be detected before hatch, although a prominent tectal labeling pattern throughout most layers was evident. After hatch, both neurotrophins and their receptors showed a dramatic increase of retinal and tectal distribution. While the tectal and retinal protein synthesis of NT-3 vanished after 2 weeks, that of BDNF could still be revealed in adults. Therefore, the establishment of the retino-tectal system does not seem to depend on these neurotrophins before hatch, although they are probably utilized to shape the intratectal wiring pattern. In contrast, BDNF and NT-3 could play a prominent role in posthatch retino-tectal plasticity, as the consolidation of tectal asymmetries requires posthatch modifications of tectal circuits and proceeds within the first two posthatching weeks. These data are comparable with the distribution of neurotrophins in the retino-tectal system of chicks, although the onset of neurotrophin synthesis seems to be earlier in precocial chicks.

Animals↗

PubMed2000

Lateralization of visuospatial processing in the bottlenose dolphin (Tursiops truncatus).

Two adult female bottlenose dolphins were tested for cerebral asymmetries in the visuospatial domain. The animals learned under binocular conditions a three-choice spatial discrimination task with three hoops positioned along a line in the middle of the tank. During a correct trial the dolphins had to swim from a starting position at the tanks wall through one of the hoops, come back to the starting position, choose another hoop, swim back to start and finally swim through the third hoop. For such a trial to be correct, the animals had to swim through all three hoops in any sequence without omitting or re-using one of them. After reaching criterion binocularly, monocular trials (one eye covered with an adherent suction cup) were introduced where the dolphins carried out the same task alternatingly under left or right eye seeing conditions. For both animals, the right eye performance was clearly superior to that of the left eye. Binocular and right eye performances were similar. As a result of the complete decussation at the optic nerve, this right eye superiority suggests a left-hemispheric dominance for the processing of visuospatial information. This is a remarkable deviation from the usual right hemisphere advantage for these kind of tasks found in different species of mammals and birds.

Animals↗

PubMed2000

Asymmetry pays: visual lateralization improves discrimination success in pigeons.

Functional cerebral asymmetries, once thought to be exclusively human, are now accepted to be a widespread principle of brain organization in vertebrates [1]. The prevalence of lateralization makes it likely that it has some major advantage. Until now, however, conclusive evidence has been lacking. To analyze the relation between the extent of cerebral asymmetry and the degree of performance in visual foraging, we studied grain-grit discrimination success in pigeons, a species with a left hemisphere dominance for visual object processing [2,3]. The birds performed the task under left-eye, right-eye or binocular seeing conditions. In most animals, right-eye seeing was superior to left-eye seeing performance, and binocular performance was higher than each monocular level. The absolute difference between left- and right-eye levels was defined as a measure for the degree of visual asymmetry. Animals with higher asymmetries were more successful in discriminating grain from grit under binocular conditions. This shows that an increase in visual asymmetry enhances success in visually guided foraging. Possibly, asymmetries of the pigeon's visual system increase the computational speed of object recognition processes by concentrating them into one hemisphere while preventing the other side of the brain from initiating conflicting search sequences of its own.

Animals↗

PubMed2000

Lateralized interhemispheric transfer of color cues: evidence for dynamic coding principles of visual lateralization in pigeons.

Visual feature discrimination tasks in pigeons reveal a right eye/left hemisphere dominance at the population level. Anatomical studies and lesion data show this visual lateralization to be related to asymmetries of the tectofugal system, which ascends from the tectum over the n. rotundus to the forebrain. Anatomically, this system is characterized by numerous morphological and connectional asymmetries which result in a bilateral visual representation in the dominant left hemisphere and a mostly contralateral representation in the subdominant right hemisphere. Ontogenetically, visual lateralization starts with an asymmetrical embryonic position within the egg, which leads to asymmetries of light stimulation. Differences in exposure to light stimulation between the eyes result in activity differences between the ascending tectofugal pathways of the left and the right hemisphere, which are transcribed during a critical time span into morphological asymmetries. The asymmetries established after this transient period finally start to determine the lateralized processes of the visual system for the entire life span of the individual. We now can show that these anatomical lateralizations are accompanied by asymmetries of interocular transfer, which enable a faster shift of learned color cues from the dominant right to the left eye than vice versa. In summary, our data provide evidence that cerebral asymmetries are based both on "static" anatomical and on "dynamic" process-dependent principles.

Animals↗

PubMed2000

Steroid fluctuations modify functional cerebral asymmetries: the hypothesis of progesterone-mediated interhemispheric decoupling.

This study examines the modulation of functional cerebral asymmetries by gonadal hormones in three distinct groups. Young, normally cycling women performed a prototypical left (lexical decision) and two prototypical right-hemispheric tasks (figural comparison and face discrimination) during the low steroid menses and the high steroid midluteal phase. Saliva progesterone levels were measured with radioimmunoassay (RIA). Parallel to younger females, young men, and postmenopausal women were tested at matching time intervals. Results revealed significant interactions between cycle phase and visual half-field in the accuracy of all three tasks for the younger women; stronger lateralization patterns occurring during menses, while a more bilateral or at least less asymmetric cerebral organization predominated the midluteal phase, when highest levels of progesterone appear. Progesterone seemed to have a significant influence on lateralization in the figural comparison task, with high hormone levels enhancing the performance of the left hemisphere (for this task subdominant), thereby decreasing asymmetry. After menopause, when the levels of gonadal hormones are lower and more stable, the lateralization patterns for all three tasks were similar to those of men and normally cycling women during menses. These results make it likely that steroids and especially progesterone are able to reduce cerebral asymmetries. We hypothesize that progesterone attenuates the effect of glutamate on non-NMDA receptors. This could diminish cortico-cortical transmission which is mostly dependent on a glutamate-induced initial EPSP in pyramidal neurons which receive transcallosal input. The reduction in callosal transfer could then suppress the functional asymmetries.

Adult↗

PubMed2000

Visual lateralization of pattern discrimination in the bottlenose dolphin (Tursiops truncatus).

The aim of the present study was to investigate whether bottlenose dolphins have cerebral asymmetries of visual processing. The monocular performance of the adult dolphin Goliath was tested using a large number of simultaneous multiple pattern discrimination tasks. The experiments revealed a clear right eye advantage in the acquisition and the retention of pattern discriminations as well as asymmetries in the interhemispheric transfer of visual information. As a result of a complete decussation at the optic nerve, this right eye superiority is probably related to a left hemisphere dominance in visual processing.

Animals↗