American cockroaches: joint action of DDT and Ouabain on adults.
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Animals↗
Biomedical subjects
O Giannotti
Publications and source records attributed to O Giannotti.
[Kinetics of the binding 14C-DDT-and haemolymph lipoproteins of Periplaneta americana (author's transl)].
A study of the kinetics of the complexation of the 14C-DDT and lipoproteins of the haemolymph of Periplaneta americana (L.) was made, the method employed was that described by WINTER et alii (7). It was found that the above complex was unstable when kept at low temperatures (5 degrees) for more than twenty four hours; the incorporation of 14C-DDT in the proteic fraction increased with temperature up to 42 degrees C, and at least 6 hours of incubation were necessary to bind the 14C-DDT to the lipoprotein.
Animals↗
[Alteration of the ATP-ase Mg++ and Mg++ Na+ K+ activities of the central nervous system of Periplaneta americana L. after sonification. (author's transl].
A prolonged ultrasonic homogenization of a Periplaneta americana L. suspension of ganglia in a buffer solution pH 7,6 when compared with a Potter Elvehjem homogenization shows a marked reduction of Mg++ and (Mg++ + Na+ + K+) ATPase activities. Mg++ ATPase was not modified while the (Mg++ + Na+ + K+) ATPase was completely inhibited after 30 second ultrasonic homogenization. This probably results from an enzyme structure alteration caused by the ultrasonic treatment.
Adenosine Triphosphatases↗
[GLC method for microdetermination of catecholamines (author's transl)].
A gas liquid chromatographic method for determination of catecholamines in small sample is described. The method involves homogenization of biological material into 0,1 M formic acid 5 mM ascorbic acid and subsequent conversion in their trimethylsilyl derivatives with BSA reagent and determined on 3% OV-1 column in a isothermal condition at 220 degrees C with the cromatograph equipped with hydrogen flame ionization. With respect to specificity, reproducibility and recovery, the results were satisfactory.
Catecholamines↗
[Study of catecholamines in the thoracic ganglia and sixth ganglion by gas liquid chromatography (author's transl)].
The gas liquid chromatographic method was used in the investigation of catecholamines in the central nervous system of Periplaneta americana. There is evidence of existence of noradrenaline in the thoracic ganglia and in the sixth abdominal glanglion. Noradrenaline might be the neurotransmitter substance in the nervous system of this insect. Besides noradrenaline, some compounds are present in the nervous system, with different retention times from 1-adrenaline, dopamine and homovanillic acid.
Animals↗
[Peripherial ATP-ases in the different components of the central nervous system of Periplaneta americana L. (author's transl)].
A smaller ATP-ase activity in the abdominal nerve cord of the Periplaneta americana L. was observed when compared by peripheral area with ganglia activity of the central nervous system which have more complex physiological functions. There was no significant difference in the enzyme distribution in the pro, meso, metatoraxic, subesophageal ganglia and the sixth abdominal ganglion.
Adenosine Triphosphate↗