PubMed Health⌕ Search

Biomedical subjects

O Glatter

Publications and source records attributed to O Glatter.

5 recordsLinked to original sources
PubMed1992

Shape change of human blood platelets: reliable and fast detection by quasi-elastic light scattering.

Quasi-elastic light scattering has been used for the first time to obtain reliable information about the morphology of platelets under physiological conditions within a short time. By measuring two independent parameters (electrophoretic mobility and diffusion coefficient) it is possible to distinguish between different stages of shape change on the one hand, and between shape change and binding of particles to the platelet surface without shape change on the other hand.

Adult↗
PubMed1978

The structure of human-plasma low-density lipoprotein B. An X-ray small-angle scattering study.

1. X-ray small-angle scattering of human plasma lipoprotein B of the low-density fraction (rho = 1.016--1.060 g.cm-3) has been recorded to high precision at different electron density contrasts. 2. The overall structure of the particles is characterized by a quasi-spherical shape and radial symmetry. A maximum diameter of 23 nm and a molecular weight of 2.4 X 10(6) have been determined. 3. The internal structure is described in terms of a model consisting of spherical layers with different electron densities indicating that the neutral lipids are arranged in the core of the molecule up to a radius of about 8 nm surrounded by a monolayer of free cholesterol, phospholipids and protein. The neural lipids are shown to be in an ordered, liquid crystalline state at 4 degrees C and to undergo a thermotropic transition into a disordered state at higher temperatures.

Cholesterol↗
PubMed1977

The lipid bilayer structure of the abnormal human plasma lipoprotein X. An X-ray small-angle-scattering study.

The structure of the abnormal lipoprotein X occurring in the plasma of patients with obstructive jaundice was investigated by X-ray small-angle scattering. The data were analyzed by discussing the distance distribution functions obtained directly from the experimental data by Fourier transformation, involving no a priori assumptions. The results provide evidence for lipoprotein X being essentially a random distribution of lamellae with a thickness of 5.1 nm and are consistent with hollow spherical (vesicular) structures of outer diameters greater than 30 nm with some overall size heterogeneity. Under the experimental conditions chosen, lipoprotein concentrations between 0.01 and 0.18 g/ml in in buffers of low ionic strength, lateral stacking as observed in negative-stain electron microscopy does not occur. The electron density profile perpendicular to the lamellar plane indicates that a lipid bilayer is the underlying structural principle, with the protein moieties partly bound within the polar head-group regions and partly occluded in soluble form in the vesicle interior.

Cholestasis↗