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Biomedical subjects

O Haferkamp

Publications and source records attributed to O Haferkamp.

At least 19 recordsLinked to original sources
PubMed1992

A fluorescence-microscopic and flow-cytometric study of HeLa cells with an experimentally induced respiratory deficiency.

HeLa cells, cultured over a long period in a medium containing low doses of ethidiumbromide, were used as a model system for flow-cytometric detection of human cells with impaired mitochondrial respiratory function. Based on laserscan and flowcytometric analysis after rhodamine 123 staining, the mitochondrial membrane potential of respiratory deficient cells seems unchanged as compared to control cells. Maintenance of this membrane potential in respiration-impaired cells requires glycolytic ATP generation, as transient inhibition of glycolysis by sodium fluoride affects rhodamine 123 accumulation in ethidiumbromide-treated cells, but not in control cells. We present a protocol which allows the detection and separation of respiratory deficient cells by flow cytometry.

Adenosine Triphosphate↗
PubMed1991

Functional and molecular analysis of mitochondria in thyroid oncocytoma.

We report a functional and molecular analysis of nine oncocytic tumors of the human thyroid. In all the abundance of mitochondria observed ultrastructurally was accompanied by an increase in enzymatic activities of respiratory complexes 1 (NADH dehydrogenase), 11 (succinate dehydrogenase) IV (cytochrome c oxidase), and V (ATPase). Western blot analysis failed to detect uncoupling protein in the tumors. The elevated respiratory enzyme activities were paralleled by an increase in the mitochondrial DNA content. Restriction analysis of mitochondrial DNA gave no indication of heteroplasmy or other gross alterations. We conclude that the mitochondrial proliferation in oncocytic tumors is probably not the result of a compensatory mechanism for the deficiency in enzyme complexes of the mitochondrial respiratory chain.

Adenoma↗
PubMed1991

[Interactions of fluconazole and phagocytic cells].

Fluconazole is a triazole compound with a coefficient of distribution P at pH 7.4 of 1.6 (log P = 0.2), and has thus both hydrophilic and lipophilic properties. The physicochemical and pharmacokinetic profiles of fluconazole are clearly different from those of other azole antimycotics. 3H-labelled fluconazole penetrates very rapidly into granulocytes and monocytes (macrophages) isolated from volunteers. The concentrations of the triazole in the granulocytes are about 28% and in the macrophages about 63% higher than in the extracellular milieu. At the concentrations examined (5, 10 and 20 micrograms ml-1) fluconazole damages cells of Candida albicans which have been phagocytized by granulocytes or by macrophages. A clear synergy between fluconazole and the phagocytes can be demonstrated.

Candida albicans↗
PubMed1979

[Prognostic aspects of early gastric carcinoma (author's transl)].

The results of treatment of early gastric carcinoma were analysed in 65 patients. In 33 patients the operation was performed 5 years ago. The 5-year-survival rate was 70%. Prognosis was predominantly influenced by the histology of the tumour. Signet ring cell and anaplastic early carcinomas (diffuse type) had a worse prognosis than intestinal (differentiated) forms. Carcinomas localised wholly in the mucosa had no better prognosis than those penetrating into the submucosa. There is no choice of surgical method according to prognostic criteria as the diagnosis "early gastric carcinoma" can be made only postoperatively from the resection material. Bioptic proof of carcinomatous gastric mucosal changes requires full observation of the rules of cancer surgery.

Biopsy↗
PubMed1979

Gastritis, intestinal metaplasia and dysplasia versus benign ulcer in stomach and duodenum and gastric carcinoma -- a histotopographical study.

In histological examination of gastrectomy specimens from patients with duodenal ulcer, gastric ulcer, and early and advanced cancer, both chronic atrophic gastritis and intestinal metaplasia were identified in 54% of the cases with duodenal ulcer. At 90 to 100%, respectively, these mucosal changes were approximately twice as frequent with gastric ulcer and early and advanced gastric cancer. Mild dysplasia occurred in 54% of the cases with duodenal ulcer; occurred somewhat more frequently with gastric ulcer, in 75% of the cases; and in almost all cases with early and advanced gastric cancer, at 90% and 100%, respectively. Whereas 27% of the cases with duodenal ulcer, 62% with gastric ulcer, and 90% and 95% of the respective cases with early and advanced gastric cancer showed moderate dysplasia, only severe dysplasia in early gastric cancer (40%) and advanced gastric (81%) was clearly more frequent in comparison to duodenal ulcer (9%) and gastric ulcer (12%). In the cases with duodenal ulcer chronic atrophic gastritis and intestinal metaplasia were limited mostly to the antrum; with gastric ulcer and cancerous stomach disorders, they also occurred in other stomach sections. Mild and moderate dysplasia conformed to the same distribution pattern. Severe dysplasia, which was only detected in two ulcer cases, was not only substantially more frequent in cases with early and advanced gastric cancer, but also showed a clear topographic relationship to cancer localization in the stomach.

Aged↗
PubMed1978

[The malignant fibrous histiocytoma of bone (author's transl)].

The primary malignant fibrous histiocytoma of bone (Stout) is a rare and autonomous sarcoma of the bone which has to be separated from the osseous sarcoma and the osseous fibrosarcoma. The malignant histiocytoma is morphologically characterized by the storiform pattern of interlacing spindle cell bundles and functionally by the phagocytosis of lipids, glycogen, hemosiderin and hematoidin. Contrary to the osteosarcomas, the main age of osseous histiocytomas are the fifth and sixth decade. Location of predilection are the metaphyses of the long tubular bones. The X-rays show moth-eaten spongiolysis, endosteal erosion and perforation of the cortex. Early metastases in the lungs are frequent. The primary treatment is a surgical one. Case record of a typical malignant osseous histiocytoma from the proximal metaphysis of the left femur with metastases in the lung of a female child, aged 14 years.

Adolescent↗
PubMed1978

The cervical lymph nodes in Streptococcus pyogenes, group A, type 50, infection in mice.

Streptococcus pyogenes, group A, type 50, one of the few group A streptococcal types naturally occuring in mice, proved highly virulent in this species after experimental infection. Intranasal infection of 96 mice (Swiss albino, NMRI, and CBA) with this microorganism induced profound reactions in the cervical lymph nodes of 69% of the animals. Histologically, two different forms of reaction were distinguishable. In 61 mice, the lymph nodes exhibited follicular and lymphoplasmacellular hyperplasia and in 9 animals suppurative lymphadenitis was present. The ability of type 50 streptococci to persist in the pharynx of mice, and the similarity of the morphological changes induced by this organism appear to make intranasal murine group A, type 50, streptococcal infection a suitable model for human streptococcal pharyngitis.

Animals↗
PubMed1978

Immunomorphology and pathogenesis of echinococcosis. III: Microscopic immunofluorescence studies in experimental alveolar echinococcosis.

By application of immunohistological methods in experimental alveolar echinococcosis, detailed information was obtained on the antigenic properties of each component of the echinococcus cyst. It was established that of the various layers, the capsula germinativa of the parasite cyst possesses the strongest antigenicity regardless of localization and maturity of the cysts. The dissemination of echinococcal antigens within the internal organs of the experimental animals was found to follow the general trends of other infections. Substances obtained from the capsula germinativa appear most suitable as reagents for immunodiagnostic purposes due to their strong antigenicity.

Animals↗
PubMed1977

Phagocyte dysfunction in common variable immune deficiency.

The history of a 13-year old boy is reported who suffered from frequent bacterial, enteroviral, and protozoal infections since late infancy. A decrease in the serum levels of IgG2, IgG3, IgA, a neutrophil dysfunction, and a partial cellular immune deficiency could be demonstrated. A deficiency of folic acid produced a pancytopenia which enhanced the patient's susceptibility to infections. The combined substitution of gammaglobulins and folic acid only was able to break this vicious cycle.

Adolescent↗