Biomedical subjects

O Iu Kuznetsov

Publications and source records attributed to O Iu Kuznetsov.

4 recordsLinked to original sources

PubMed1987

[Heterogeneity of Shigella flexneri during selective synchronization].

The study of a number of main growth characteristics of S. flexneri (S) cells during the first and second cycles of their cultivation in a solid culture medium in Peshkov's chamber from seed material, nonsynchronized and synchronized by C. E. Helmstetter's method, has revealed that this method does not permit obtaining completely synchronized cultures. Four physiological cell clusters, differing in the time of generation, growth rate, size at the beginning and the end of the cycle and the time of adaptation, have been detected.

Bacteriological Techniques↗

PubMedDate not supplied

[Choice of informative characteristics for the characterization of the adaptive process in the population of rod-shaped bacteria].

Different data characterizing the growth and development of rod-shaped bacterial cells (Shigella flexneri strains S and Rd) at the initial stage of the formation of their populations on a solid culture medium were analyzed with a view to the evaluation of the adaptive process. The study revealed that Lo and Lk (initial and end of bacterial lenght) and tau (generation time) of cells were the optimal informative characteristics. The determination of these characteristics on the cell population level showed the specific features of the adaptive process of the bacterial cultures. The ratio Lk/Lo of the population cells characterized the morphological (funtional) structure of the population. It can be regarded as the structural adaptive index of the population. The determined the values of tau in the cell population reflected the adequacy (correspondence) of the intracellular state to the conditions of the environment, thus serving as indicator of the profundity of stress.

Adaptation, Physiological↗

PubMedDate not supplied

[Antimicrobial action of Lentinus edodes juice on human microflora].

The action of the juice of Shiitake mushroom (L. edodes) on pathogenic and opportunistic microorganisms, detected in cases of considerable dysbiotic changes (Escherichia coli O-114, Staphylococcus aureus, Enterococcus faecalis, Candida albicans), as well as on some bacterial eubiotic producer strains (Escherichia coli M-17, Bifidobacterium spp., Lactobacillus spp.). The juice of this mushroom at a concentration of 5% from the volume of the nutrient medium was found to produce a pronounced antimicrobial effect with respect to C. albicans, S. aureus, E. faecalis, E. coli O-114 and to stimulate the growth of E. coli M-17. Bifidobacteria and lactobacteria exhibited resistance to the action of L. edodes juice.

Anti-Bacterial Agents↗

PubMedDate not supplied

[Bacterial colony as a complex community].

Information on the adaptive behavior of cells in the process of the formation of a colony by bacteria Shigella flexneri Rd, used as a model, was obtained with the use of light, transmission and scanning microscopy. The process of the formation of a microcolony was demonstrated; at the initial stages (8 hours) it included 3 groups of cells, and 24 hours later it exhibited sharply defined structurization. The conclusion was made on the possibility of the joint use of different methods of microscopy for the study of the development of microcolonies and colonies of bacteria.

Adaptation, Physiological↗