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Biomedical subjects

O L Oke

Publications and source records attributed to O L Oke.

4 recordsLinked to original sources

Studies on weaning diets in Nigeria: 2 protein sources.

Protein isolates were extracted from melon, groundnut, and soybean cakes in an aqueous system that could potentially yield both oil and protein from oilseeds quantitatively. Protein isolates obtained were 91.2, 78.6 and 79.5% of the total protein content of melon, groundnut and soybean cakes respectively. Both cakes and isolates were assessed in a rat bioassay while breadfruit, cassava, and corn starch were also investigated as sources of carbohydrate. Results showed that there were no significant differences between feed intake, weight gain and PER of rats fed the cake and the protein isolates except in soybean. Weight gain, PER and NPR were highest in cassava-soybean protein isolate diet followed by cassavacasein diet (control) while performance was only moderate with melon- and groundnut-cassava diets. With melon protein isolate, cassava and breadfruit were better carbohydrate sources than corn starch. In simulated weaning diets, cassava-sucrose-melon-soybean protein isolate based diet performed better (weight gain 33.4 g, PER 1.63, NPR 2.23) than a commercial weaning food (weight gain 30.6 g PER 1.57, NPR 2.18) while replacement of sucrose and cassava at 30% by breadfruit yielded a cost effective diet with good performance (weight gain 29.4 g, PER 1.51, NPR 2.12). Diets where soybean protein served as the sole source of protein or breadfruit, white and yellow maize 'ogi' served as the main source of carbohydrate performed only moderately.

Animals

Effects of cyanogenic agents on reproduction and neonatal development in rats.

A diet consisting of 50% gari (a form of cassava meal) was found to cause no significant biochemical and haematological changes in adult female rats. Although this diet caused a significant increase in their serum thiocyanate levels, there was no corresponding significant increase in the weight of their thyroid glands. The offspring of these rats had significantly lower birth weights and brain weights and never attained the same adult weights as those of the controls. Adult female rats fed a diet consisting entirely of raw cassava had significantly reduced haematological and biochemical indices. This diet also caused an increased incidence of cannibalism and a significant reduction in the frequency of pregnancy, the average number of the litter and the birth weights among these rats. In addition there was an increased incidence of neonatal deaths among the offspring which also had poor development, reduced brain weights and an increased tendency towards biting their litter-mates. Adult female rats fed diets containing 5 and 10 g cyanide/100 g laboratory diet survived for more than 3 months but never became pregnant. They developed enlarged thyroid glands and tumours of the large intestine. The relevance of these findings to the areas of the world where cyanogenic glycosides form the staple diet is discussed.

Animals