PubMed Health⌕ Search

Biomedical subjects

O Lüderitz

Publications and source records attributed to O Lüderitz.

At least 55 records · Page 3Linked to original sources
PubMed1975

Nature, type of linkage, and absolute configuration of (hydroxy) fatty acids in lipopolysaccharides from Xanthomonas sinensis and related strains.

The fatty acids present in lipopolysaccharides from Xanthomonas sinensis were identified as decanoic, 9-methyl-decanoic, 2-hydroxy-9-methyl-decanoic, 2-hydroxy-9-methyl-decanoic, D-3-hydroxy-decanoic, D-3-hydroxy-9-methyl-decanoic, D-3-hydroxy-dodecanoic, and D-3-hydroxy-11-methyl-dodecanoic acid. These fatty acids occur in the lipid A component where they are bound through ester and amide linkages to glucosamine residues. All types of fatty acids are ester bound; however, part of D-3-hydroxy-dodecanoic and D-3-hydroxy-11-methyl-dodecanoic acid is also involved in amide linkage. The hydroxyl groups of ester-linked 3-hydroxy fatty acids are not substituted. Similar fatty acid patterns were obtained from lipopolysaccharides of nine other Xanthomonas species.

Chemical Phenomena↗
PubMed1975

Chemical structure of lipopolysaccharides and endotoxin immunity.

Lipopolysaccharides (endotoxins) of gram-negative bacteria consist of 2 components with distinct physico-chemical character: a heteropolysaccharide and a covalently linked lipid, termed lipid A. The chemical structure of lipid A, which represents the toxic center of lipopolysaccharides, is discussed. Evidence is presented that lipid A antiserum suppresses the pyrogenic effect of lipid A and lipopolysaccharides in rabbits. The protective power of lipid A antiserum, however, is only expressed in animals which have been pretreated with lipid A or lipopolysaccharide indicating that other than humoral factors, perhaps cellular, also participate in endotoxin fever (cross) immunity. The fever resistance mediated by lipid A antiserum seems to be endotoxin-specific with regard to both the preparative and the challenging injection. Lipid A antiserum therefore may serve as a tool to discriminate between fever caused by endotoxins and that induced by other pyrogens.

Absorption↗
PubMed1973

The mitogenic effect of lipopolysaccharide on bone marrow-derived mouse lymphocytes. Lipid A as the mitogenic part of the molecule.

Lipopolysaccharides with different structure, isolated from different mutant strains of Escherichia coli and Salmonella bacteria, and chemical degradation products of these lipopolysaccharides have been employed to investigate which part of the lipopolysaccharide molecule exerts mitogenic effects on bone marrow-derived mouse lymphocytes. Within the structure of lipopolysaccharide consisting of lipid A, a core polysaccharide, and the O-polysaccharide antigen, lipid A was found to be the mitogenic part. The mitogenic effect of lipid A, consisting of phosphorylated glucosamine disaccharide units with ester- and amide-linked fatty acids, was lost after alkali treatment, which removes ester-linked fatty acids. Insertion of the lipid A portion of lipopolysaccharides into the lipid bilayer of the plasma membranes of bone marrow-derived lymphocytes is discussed as the initial mitogenic action.

Antibody Formation↗
PubMed1973

Pyrogenicity and immunogenicity of lipid A complexed with bovine serum albumin or human serum albumin.

The lipid A component of bacterial lipopolysaccharides (endotoxins), when complexed to bovine serum albumin (BSA) or human serum albumin (HSA), was shown to be a potent pyrogen. Furthermore, rabbits could be protected against endotoxin fever by immunization with both lipid A.BSA and lipid A.HSA complexes. The results presented in this paper show that lipid A is responsible for the pyrogenic activity of endotoxins and their ability to induce pyrogenic immunity.

Animals↗