[Absence of correlation between morphometric parameters and C-erb-B2 positivity].
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Biomarkers, Tumor↗
Biomedical subjects
O Leopardi
Publications and source records attributed to O Leopardi.
[Validity of the AgNOR count in cervical pathology].
Nucleolar organizer regions (NORs) were counted on ten cases each of normal ectocervix, CIN 1, 2 and 3 to verify the possibility of a differentiation between the various grades of CIN and between them and condylomata. Counts were performed on the full thickness of the tissue, layer by layer (stratified counts). A significant difference (p < 0.05) was found between the means of normal tissue toward condylomata and CIN 2 and 3 and between CIN 1 and CIN 2 and 3. There was no significance (p < 0.05) between normal tissue and CIN 1, between CIN 2 and 3 and between condylomata and CIN 2 and 3. The range of variations on the counts was associated with overlapping between the various cases. Our data showed also a progressive rise in mean NOR values from normal tissue to CIN 3. The stratified counts showed in all the groups a rise from basal to parabasal cells. Counts on parabasal and intermediate layers distinguished two groups of cases. In one there was either the same number of dots or a further rise from one layer to the next, while in the other a definite decrease was seen. The former pattern may be related to a potential for malignant evolution of the lesion. NORs should be counted in all cases of CIN and condylomata to treat more aggressively those lesions which present the pattern of a progressive rise of NORs from basal to intermediate cells.
Carcinoma in Situ↗
[The quantitative study of nucleolar organizers (AgNOR) in the diagnosis of laryngeal dysplasias and in the assessment of the prognosis in invasive carcinoma].
Nucleolar organiser regions (AgNOR) are chromosomal segments in which ribosomal RNA is codified. These structures can be identified in the nuclei of cells in routine histological sections using a silver staining technique which reveals the proteins associated with transcription RNA. The number of AgNOR identified depends on the state of cell proliferation. The purpose of the present study was to evaluate the distribution of AgNOR in normal laryngeal mucosa, dysplastic and neoplastic, in order to establish a possible correlation with the degree of dysplasia and/or the entity of the malignant lesions. Ten slides each of specimens of laryngeal carcinoma, serious dysplasia and middle dysplasia were prepared with the silver staining technique. A count of the NOR in the basal, parabasal, intermediate and superficial levels was made. Findings show an increase in the average value of NOR proceeding from normal tissue to carcinoma in situ and malignant carcinomas. The number of AgNOR was lower always than that observed in the parabasal level. In the intermediate cell section the number of AgNOR was inferior to that in the parabasal level in normal tissue as well as in middle dysplasia, while it remained high in serious dysplasia and in carcinomas, thus indicating a great deal of biological activity in these cases. This method can be employed in order to better individualize those dysplastic lesions which, because of their tendency towards malignancy, must be kept under observation by means of an extremely accurate follow-up.
Carcinoma↗
[Evaluation of sensitivity and specificity of various staining methods for the detection of myocardial fibrosis].
Fifty bioptic endomyocardial samples from 15 patients with idiopathic dilatative cardiomyopathy have been stained with PicroSirius, Masson-Goldner, Van Gieson Verhoeff, AFOG. The extension of stained areas has been evaluated by morphometry using a videoscan. As control sections samples have been taken from lower third of the right ventricle at autopsies of healthy subjects dying in road accidents. Morphometric analysis of subendocardial interstitial and perivascular fibrosis has been performed. For the authors the most useful stain in the evidentiation of fibrosis was PicroSirius both in the bioptic samples and the autoptic ones.
Adolescent↗
[Utility of morphometry and nucleolar organizer regions count in the differentiation of reactive and malignant mesothelial cells in pleural effusions].
The authors have evaluated in eight reactive and eight malignant pleural effusions the number of intranucleolar dots representing the nucleolar associated proteins stained with silver colloid technique (interphase nucleolar organizer regions-AgNOR). The mean number per nucleus in benign reactive mesothelium was significantly lower than in mesothelioma effusions. Other morphometric measurements of nuclear diameters and areas were effected. The authors evaluate the variability of the AgNOR counts and morphology in reactive and mesothelioma cells of pleural fluids.
Humans↗
[Anatomo-clinical presentation of a case of asplenic syndrome and dextrocardia].
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Abnormalities, Multiple↗
[Morphometric evaluation and AgNOR number in c-erbB-2 positive and negative breast carcinoma: absence of correlation].
A total of 722 nuclei from 22 fine needle aspirates (FNA) of breast carcinomas have been subjected to quantitative nuclear analysis. Form Factor, Nuclear Area and Nuclear Major Diameter were determined for each case. An equal number of nuclei from as many aspirates from benign breast nodules was used as control. The aim of the study was to evaluate the predictive value of these parameters on the result of the immunohistochemical determination of the c-erb B-2 antigen in the tumour mass, since 6 of the 22 carcinomas were positive to this antigen. None of these parameters singly or in combination proved of any value in predicting the positive or negative result of the c-erb B-2 antigen determination. Nuclei both positive and negative for the antigen were located within the large cell carcinomas. Nucleolar Organizer Regions were also determined on the histologic sections of both groups to assess any statistically significant difference not only between controls and carcinomas, but also between c-erb B-2 positive and negative cases. Both NOR mean numbers and NOR cytograms were of no use, since no difference was found between c-erb B-2 positive and negative cases.
Breast Neoplasms↗
[Nucleolar organizer regions of benign pathology of the parathyroids].
Nucleolar organizer regions (NOR) have DNA loops containing genes that encode for ribosomal RNA; some proteins located near the NORs are argyrophilic and by Ploton's one step method we could quantify the NORs in sections of paraffin embedded tissues. We have evaluated the number of intranuclear dots in 46 parathyroid glands from 18 parathyroidectomies. The glands were weighed and the stromal fat was evaluated by a point-counting method. AgNORs were counted in 100 cells from each gland. The mean count was 3.03 in six adenomas (A), 2.39 in six cases of primary hyperparathyroidism (PH) and 2.63 in six cases of secondary hyperparathyroidism (SH). Statistical analysis performed by Mann-Whitney U test showed that differences between A and PH groups were significant. The difference between A and SH was also significant. The residual parathyroid tissue surrounding the adenoma had a significantly lower AgNOR mean count than adenomatous cells. The AgNOR mean number can be useful in assessing normal or suppressed parathyroid tissue.
Adenoma↗