PubMed Health⌕ Search

Biomedical subjects

O M Moiseeva

Publications and source records attributed to O M Moiseeva.

16 recordsLinked to original sources
PubMed2006

[Cardial fibrosis and the functional activity of leukocytes in patients with essential arterial hypertension].

The study revealed an increase in the serum levels of TGF-beta1 and the N-terminal peptide procollagen type III in patients with essential arterial hypertension (EAH), as well as an association between this increase and the duration of the disease, mean day arterial pressure profile, and left ventricular hypertrophy. An increased leukocyte functional activity is associated with disturbances in left ventricular diastolic function and an increase in TGF-beta1 serum concentration in EAH. The authors conclude that leukocytes participate in the development of myocardial hypertrophy and cardial fibrosis through the secretion of pro-inflammatory cytokines and peptide growth factors within the process of their activation.

Adult↗
PubMed2006

[Activation of mast cells after experimental myocardial infarction in 3 week-old rats].

It is known that mast cells (MC) take an active part in regeneration processes in postinfarction heart in adult rats and humans. Behaviour of population of cardial MCs has been studied 20, 60, 75 and 90 days after experimental myocardial infraction induced in 3 week-old and adult rats by ligation of left coronary artery. The density of MC of different degrees of maturity was estimated in atrium and ventricle on paraffin sections stained with Alcian blue - Safranin. Findings were compared with MC density obtained in hearts of intact rats. The MC density in intact 1.5-2.5 month-old rats in atrium and ventricle was about 0.6 cells/mm2, in intact 3.5-4.0 month-old rats in atrium--1.2 cells/mm2, in ventricle--0.6 cells/mm2. The MC density in 3 week-old rats with infarction was significantly higher than in intact rats: 5-fold increase in 20 and 60 days in atrium, and 2-fold increase in 60 and 75 days in ventricle. In 60 days after infarction the MC density in adult rats was 3 times lower in atrium and 2 times lower in ventricle than in the same heart compartments of 3 week-old rats with infarction. After infarction in 3 week-old rats, a relative share of young cells with alcian-positive granules sharply increased in 20 days and then decreased by 60-75 days. This indicates a migration of immature MCs to infracted myocardium and their subsequent differentiation. The MC activation after infraction in young rats may result from a more active immune reaction in younger rats and/or functional peculiarities of their MC.

Aging↗
PubMed2005

[Non-hemodynamic effects of calcium channel blocker nifedipine: effects on functional activity of blood cells and endothelium].

AIM: To compare efficacy of long-acting nifedipin (cordipin-XL) and beta-blocker atenolol (tenormin) in hypertensive patients; to assess efficacy of antihypertensive treatment on functional activity of blood and endothelial cells, remodeling of the myocardium and vessels. MATERIAL AND METHODS: A total of 35 patients with essential hypertension stage II entered the trial. Eighteen of them received cordipin-XL, 17--tenormin. RESULTS: Contrary to tenormin, cordipin-XL significantly improved blood rheology, reduced functional activity of leukocytes, concentration of Willebrand factor and intercellular adhesive molecules in blood plasm. Positive hemodynamic and non-hemodynamic effects of cordipin-XL were accompanied with reversion of left ventricular hypertrophy. CONCLUSION: Non-hemodynamic effects of cordipin-XL associated with improvement of endothelial function and blood rheological properties seem to have an independent role in prophylaxis of cardiovascular complications.

Antigens, CD↗
PubMed2004

[Heat shock proteins 70 kD as a new marker of lesions to target organs in hypertension].

The presence and concentration of the inducible form of heat shock proteins (Hsp70) were determined in blood serum of patients with hypertension (HT), stage II, including 28 males with stage II HT, who did not receive any antihypertensive therapy, and 10 healthy subjects. A higher Hsp70 content was found in blood serum of in patients with stage II HT. Such Hsp70 concentration was related with ECG signs of left ventricular hypertrophy and with the rennin content in blood. The findings of endothelium-dependent vasodilation (EDVD) of the radial artery did not differ within the examined groups. Still EDVD was reliable lower in patients with atherosclerotic plagues in the common carotid arteries. A reduced EDVD in the radial artery was attributed to a higher EDVD content in blood serum, which did not depend on a prescription or on an arterial hypertension severity. The results are indicative of that a higher inducible Hsp70 concentration in blood serum could potentially be a marker of lesions to target organs in HT; equally it could be of a certain significance in the atherosclerotic pathogenesis.

Biomarkers↗
PubMed2004

[Rheological properties of blood and endothelial function in patients with hypertensive disease].

Patients with hypertension are characterized by elevated platelet and erythrocytes aggregation and presence of relationship between rigidity of red blood cells and myocardial mass index. They also have increased number of leukocytes in peripheral blood, enhanced adhesive ability of neutrophils, increased representation of integrin receptors on lymphocytes and monocytes, and expression of Fas-receptors. These features evidence for augmented functional activity of leukocytes in hypertensive disease. Changes of rheological parameters of blood occur before impairment of vasomotor endothelial function. Augmentation of viscous properties of blood and functional activity of leukocytes aggravates hemodynamic disturbances in hypertension and can facilitate myocardial and vascular remodeling.

Blood Platelets↗
PubMed2004

[Clinical value of morphological analysis of radial and internal mammary arteries in patients with ischemic heart disease].

Morphological study of intraoperative biopsies of radial and internal mammary arteries taken from patients with ischemic heart disease during coronary bypass surgery revealed structural alterations of intima and media mostly in radial artery. These alterations depended on severity of arterial hypertension, presence of diabetes and extent of atherosclerosis. Hyperplasia of intima could be a basis for radial artery spasm and cause of graft stenoses and occlusions. Therefore protocols of pre and post operative care should take into consideration individual risk factors and morphological and functional features of an artery used for grafting.

Coronary Artery Bypass↗
PubMed1998

[Modelling of myocardial hypertrophy in vitro for solving problems of medicinal correction].

The work has been done on primary heart culture from neonatal rat ventricle. Cardiomyocyte hypertrophy was modelled using noradrenaline (NA), angiotensin II (AII) and fetal serum, respectively. Cell hypertrophy of primary heart cultures was assessed by measuring the surface area, the scope of protein synthesis estimated by 3H-leucine autoradiography and the contents of nucleic acids in gallocyanin-chromalum stained cardiomyocytes. The structure of myofibrillar apparatus was studied by rhodamine-conjugated phalloidin and indirect immunofluorescence of muscle alpha-actinin. Treatment with 10(-6) M NA increased 3H-leucine incorporation in 9-day old heart culture by 42% without changing cell size. AII in a dose 1 microM stimulated protein synthesis activity by 1.3 fold and the surface area by 1.7 fold, both in 2- and 9-day old primary heart cultures. The maximum stimulation of cell hypertrophy was provided by the medium supplemented with fetal serum. RNA contents in the cytoplasm of cardiomyocytes increased by 7.8 fold and the myocardial cell size by 2.9 fold in serum-supplemented culture by 9 days of cultivation. In the medium with fetal serum, amounts of cardiomyocytes with tetraploid nuclei reached 33%, against 14% in control. Coculturing of myocardiocytes and fibroblasts rendered effects of fetal serum on the growth of myocardiocytes. Cultivation in the presence of 1 microM enalapril, an ACE inhibitor, suppressed the development of cardiac muscle cells hypertrophy. The effect of enalapril depended on the degree of cellular hypertrophy. Addition of 10 microM amiloride to the medium lowered the protein synthesis by 29% independently on the initial cellular hypertrophy.

Adrenergic alpha-Agonists↗
PubMed1997

[Comparative study of the level of cardiomyocyte hypertrophy in patients suffering from ischemic heart disease with normal arterial pressure and in hypertension].

A study was performed on the human atrial myocytes, isolated by means of alkaline dissociation of bioptates, obtained in clinics during open heart surgery, from two groups of patients. The first group consisted of 8 men aged from 40 to 62 years, with ischemic heart disease and normal arterial blood pressure. The second group involved 10 men, aged from 43 to 67 years, with ischemic heart disease and arterial hypertension. The nuclear DNA and the total protein in the cytoplasm of isolated cardiomyocytes were revealed by means of the two consecutive tests: the Feulgen and Naphthol yellow S staining. DNA and protein contents were determined by two wave-length scanning cytophotometry. It is ascertained that the temperate arterial hypertension exerted no appreciable influence on the polyploidy level of atrial myocytes, which correlated with the age of patient; in both groups (coefficients 0.7 and 0.4 for the first and second groups, resp.). The myocyte areas correlate with their ploidy (coefficients--0.7 and 0.7, accordingly). The protein contents in myocytes also correlate with their ploidy (0.5 and 0.7). The average protein content in myocytes of patients in the second group is higher than in the first group, the difference is most distinct when calculating the protein quantity per 2c-genome (717 +/- 34 a. u. and 517 +/- 51 a. u., resp.). A comparison of cytochemical and morphometric indices with some clinical findings obtained by means of echocardiography allowed to reveal the presence of correlative connections among them. The protein contents in myocytes correlate with the left ventricle mass (coefficients 0.6 and 0.5 for the first and second groups, resp.). The correlation between the rate of transmitral blood flow and myocyte ploidy (0.8 and 0.9), and the correlation between the myocyte area and the rate of transmitral blood flow (0.8 and 0.8) look convincing.

Adult↗
PubMed1990

[A method of determining erythrocyte deformability].

A simple method for determining red cell deformability is suggested, based on red cell packing during centrifugation. This method was used to study red cell deformability in patients with essential hypertension and angina of effort and found it reduced.

Adult↗
PubMedDate not supplied

[Activity of transporting ATPase and functional properties of blood cells in patients with hypertension].

Complex examination of 30 apparently healthy volunteers, 70 patients with Stages I-II hypertension and transient arterial hypertension was carried out. Impairment of blood rheologic properties and distinct decrease in the platelet activity of Na+, K+ -and Mg2+, Ca(2+)-ATPases were detected in development of hypertensive disease. In transient arterial hypertension the activity of Na+, K(+)-ATPase was increased in the erythrocyte membranes due to the fraction of old erythrocytes, while in Stage II hypertensive disease the enzymatic activity was decreased mainly in the fraction of young erythrocytes.

Adolescent↗