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O Müller-Plathe

Publications and source records attributed to O Müller-Plathe.

At least 37 records · Page 2Linked to original sources
PubMed1991

International Federation of Clinical Chemistry (IFCC), scientific division: IFCC recommendation on sampling transport and storage for the determination of the concentration of ionized calcium in whole blood, plasma and serum.

The substance concentration of ionized calcium (cCa2+) in blood, plasma or serum preanalytically may be affected by pH changes of the sample, calcium binding by heparin, and dilution by the anticoagulant solution. pH changes in whole blood can be minimized by anaerobic sampling to avoid loss of CO2, by measuring as soon as possible or by storing the sample in iced water to avoid lactic acid formation. cCa2+ and pH should be determined simultaneously.

Blood Coagulation↗
PubMed1991

Multicentre evaluation of the blood gas-electrolyte-analyser "BGE".

A multicentre evaluation of the blood gas-electrolyte-haematocrit-analyser BGE (Fa. Instrumentation Laboratory), following as far as possible the ECCLS guidelines for multicentre evaluation of blood gas analysers, was performed by three laboratories. The rules of the evaluation protocol were extended to the electrolyte and haematocrit determinations. The BGE proved to be easy to operate and maintain. The stability of the measuring system was good. The within-run imprecision of all electrodes was excellent. The same applies to the between-day imprecision, except for the calcium measurements. The systematic deviation of the gas electrodes was very small. Comparison studies revealed clinically significant deviations only for ionized calcium. Some suggestions for further improvements are made.

Blood Gas Analysis↗
PubMed1990

"Computational" blood oxygen status: requirements and limitations.

The influences of analytical variation of sO2 and pO2 measurements on the oxygen parameters p50, pO2uv-, DavO2c, CQ are examined by simulation. The greatest deviations occur when pO2 and sO2 are changing in opposite directions. p50 and pO2uv- are least sensitive against analytical variations. Differences between p50 results from arterial and mixed venous blood are discussed. The calculation of the new oxygen parameters should be restricted to blood samples with sO2 not higher than 0.96. The need for an oxygen parameter expressing the combined effects of oxygenation, Hb concentration and oxygen affinity is emphasized.

2,3-Diphosphoglycerate↗
PubMed1990

Guidelines for routine measurement of blood hemoglobin oxygen affinity. International Federation of Clinical Chemistry, Scientific Division, Committee on pH, Blood Gases and Electrolytes.

Two methods for the routine determination of blood hemoglobin oxygen affinity are described. Both methods use whole blood and do not require special equipment, tonometry or special gas mixtures. The first method consists of a one-point determination of p50, and requires only 200 microL to 400 microL of whole blood, therefore making it suitable for the pediatric population. The second method uses multiple points, thereby establishing both the shape and position of the hemoglobin oxygen equilibrium curve between 10 and 99% oxygen saturation. Interpretation of p50 is discussed in relation to evaluation of patients with hemoglobinopathies and as a parameter in estimating availability of oxygen to the tissues.

Abbreviations as Topic↗
PubMed1989

IFCC document stage 3, draft 1, dated 1989 02 01. An approved IFCC recommendation. IFCC method (1988) for tonometry of blood: reference materials for pCO2 and pO2. International Federation of Clinical Chemistry Scientific Division. Committee on pH, Blood Gases and Electrolytes.

A reference method for tonometry of blood is described. The document covers the theory of tonometry, the materials and equipment needed, and essential aspects of the tonometry procedure for blood. The partial pressures of oxygen and carbon dioxide in tonometered blood are accurately known and therefore this blood is recommended for assessing the accuracy of blood gas analyzers. Tonometry of blood samples from patients may also be used in the determination of acid-base quantities and hemoglobin-oxygen affinity, e.g. p50.

Blood Gas Analysis↗
PubMed1989

International Federation of Clinical Chemistry (IFCC), Scientific Division. Committee on pH. Blood Gases and Electrolytes. IFCC method (1988) for tonometry of blood: reference materials for pCO2 and pO2.

A reference method for tonometry of blood is described. The document covers the theory of tonometry, the materials and equipment needed, and essential aspects of the tonometry procedure for blood. The partial pressures of oxygen and carbon dioxide in tonometered blood are accurately known and therefore this blood is recommended for assessing the accuracy of blood gas analyzers. Tonometry of blood samples from patients may also be used in the determination of acid-base quantities and hemoglobin-oxygen affinity e.g. p50.

Blood Gas Analysis↗
PubMed1987

A nomogram for the interpretation of acid-base data.

A diagnostic nomogram based on log pCO2 and log cHCO3- as coordinates is presented. The significance areas for compensated acid-base disorders are indicated. The nomogram facilitates the recognition of mixed acid-base disorders. The graph is being used for on-line plotting.

Acid-Base Equilibrium↗
PubMed1984

[Total calcium or ionized calcium? A comparative study].

Determining ionized calcium after CO2 equilibration of serum with subsequent electronic conversion to pH 7.40 (Ca2+corr) gives a practicable value for the diagnosis of calcium metabolism. "Anaerobic" sampling, necessary for the measurement of the actual calcium ion concentration, is obviated. Protein or albumin concentration in serum and complex-bound calcium do not interfere. Comparison of sera from 54 patients with bone metastases or myeloma, as well as from 300 patients with other diseases, indicate that the sensitivity of Ca2+corr in the diagnosis of hypercalcaemia is about twice or three times that of total calcium and that the latter's specificity - at least when ignoring protein concentration - is unsatisfactory with 18-45% "falsely" reduced values.

Adult↗
PubMed1978

[A routine method for the investigation of the oxygen affinity of haemoglobin (author's transl)].

A method is described for the measurement of the half saturation pressure (p50) and the "standardised O2-saturation", as an index of the oxygen affinity of haemoglobin. In addition to the normal blood gas analysis, the only other parameter required is the O2-saturation after equilibration of the blood at pO2 = 31 mm Hg (4.72 kPa). The results are read from a specially prepared nomogram. Potential uses and limitations of the method are discussed.

Adult↗
PubMed1977

[A reagent-saving modification of the glucose dehydrogenase method for the determination of glucose in venous and capillary blood using the Autoanalyzer II (author's transl)].

A modification of the glucose dehydrogenase method for the Autoanalyzer II is described, which permits a considerable reduction in the quantities of enzymes and coenzyme, without affecting linearity. Using the new modification and a sample frequency of 80 per hour, glucose can be determined in venous blood and in diluted capillary blood haemolysates with no change in the construction of the apparatus.

Autoanalysis↗