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O Masamune

Publications and source records attributed to O Masamune.

At least 91 records · Page 5Linked to original sources
PubMed1994

Expression of HLA-DR antigens on colonic epithelium around lymph follicles. An anomalous expression in Crohn's disease.

The relationship between the lymph follicle and expression of HLA-DR antigens on the colonic epithelium was investigated. Colonic specimens containing lymph follicles were examined immunohistochemically for HLA-DR expression. Thirty-six biopsy specimens (29 from macroscopically uninvolved areas and seven from aphthoid lesions) were obtained from 10 patients with Crohn's disease, and another 43 specimens were obtained from 33 patients with various intestinal diseases (non-Crohn's disease). HLA-DR antigens were expressed on the epithelium around the lymph follicle in both groups. However, the rate of expression (31/36) in Crohn's disease was significantly higher than that (5/43) in non-Crohn's diseases (P < 0.001). In addition, the extent and intensity of expression were broader and stronger in Crohn's disease than in non-Crohn's disease. This study is the first demonstration of anomalous HLA-DR expression on the epithelium around the lymph follicle in Crohn's disease.

Adult↗
PubMed1994

Regulation of 60-kDa heat shock protein expression by systemic stress and 5-hydroxytryptamine in rat colonic mucosa.

Bowel dysfunction such as irritable bowel syndrome caused by stress is well described. Previous reports suggest that 5-hydroxytryptamine (5-HT) mediates alteration of bowel motility. In this study, the effects of water-immersion stress and the administration of 5-HT on the expression of a 60-kDa heat shock protein (HSP60) in rat colonic mucosa were investigated. The effect of YM-060, a 5-hydroxytryptamine 3 (5-HT3) receptor antagonist, on the expression of this protein was also studied. Water-immersion stress and the administration of 5-HT induced synthesis of HSP60 in rat colonic mucosa. The induction of HSP60 and the number of defecations were clearly inhibited by the oral administration of YM-060. Our results suggest that the induction of HSP60 in rat colonic mucosa by water-immersion stress may be associated with gastrointestinal motility mediated by 5-HT, especially via 5-HT3 receptors.

Animals↗
PubMed1994

Induction of heat shock protein and prevention of caerulein-induced pancreatitis by water-immersion stress in rats.

Water-immersion stress is known to be involved in the development of hemorrhagic pancreatitis in caerulein-induced pancreatitis, when the stress is given following caerulein injection. The effects of pre-treatment with water-immersion to caerulein-induced pancreatitis were investigated in this study. 1. A 60-kDa heat shock protein was induced by pre-treatment with water-immersion stress in the pancreas. 2. Intra-peritoneal injection of caerulein (40 micrograms/kg) induced acute pancreatitis in rats without pre-treatment with water-immersion. However, when the rats were pre-treated with water-immersion, acute pancreatitis was not developed and no change of serum amylase levels was observed by i.p. injection of caerulein.

Amylases↗
PubMed1994

Induction of HLA-DR antigen expression on human colonic epithelium by tumor necrosis factor-alpha and interferon-gamma.

BACKGROUND: We investigated the effects of tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) on HLA-DR expression on human normal colonic epithelium, using an organ culture technique. METHODS: Biopsy specimens from normal colonic mucosa were cultured for 24 h with various concentrations of TNF-alpha and/or IFN-gamma. Epithelial HLA-DR antigens were identified by an indirect immunoperoxidase staining method. RESULTS: No expression of epithelial HLA-DR antigens was observed in specimens cultured with medium alone. Concentrations of 10(3) U/ml or higher of TNF-alpha and 10(2) U/ml or higher of IFN-gamma induced expression of HLA-DR antigens in a dose-dependent manner. Expression of HLA-DR was increased in specimens cultured with both TNF-alpha and IFN-gamma compared with those cultured with either cytokine alone. CONCLUSIONS: Our findings suggest that both TNF-alpha and IFN-gamma play important roles in regulating the expression of HLA-DR on colonic epithelium in patients with inflammatory bowel disease.

Aged↗
PubMed1993

A successful case of intraarterial prednisolone injection therapy in severe ulcerative colitis unresponsive to an intensive intravenous regimen.

A successful case of intraarterial prednisolone injection therapy in severe ulcerative colitis unresponsive to conventional therapy, including an intensive intravenous regimen, is presented. After the direct administration of prednisolone into the mesenteric arteries, the patient's conditions markedly improved, and he succeeded in overcoming the acute stage and entered remission. Accordingly, he safely underwent elective surgery which was finally required because of the inability to withdraw prednisolone therapy. It is well worthwhile to consider this therapy in the last resort of medical therapy for ulcerative colitis.

Adult↗
PubMed1993

Molecular characterization of human rotavirus VP4 genes by polymerase chain reaction and restriction fragment length polymorphism assay.

A restriction fragment length polymorphism (RFLP) assay was developed to examine the genetic variability and similarity of the VP4 genes of human rotaviruses. The VP4 genes of 14 human rotavirus strains, including VP4 serotype P1A strains (Wa, P, VA70), serotype P1B strain (DS-1), serotype P2 strains (M37, 1076, McN, ST3) and serotype P3 strains (AU-1, AU228, K8, PA151, PCP5, MZ58), and those of 2 feline strains (FRV-1 and Cat2) were reverse-transcribed and amplified by the polymerase chain reaction (PCR). The amplified VP4 cDNAs were then digested with a panel of restriction endonucleases (HindIII, NruI, HaeIII, and EcoRI), resulting in the identification of at least one enzyme with which digestion produced an RFLP profile specific for a particular P serotype. Of interest was the presence of two distinct RFLP patterns within the serotype P3 VP4 genes: one corresponding to the VP4 gene carried by the members of the AU-1 genogroup and the other corresponding to the VP4 genes carried by naturally-occurring reassortants between members of the AU-1 and other genogroups.

Animals↗
PubMed1993

Sclerosing peritonitis associated with keratoconjunctivitis sicca, pleurisy, and secretory otitis media.

A male case of sclerosing peritonitis of unknown cause, associated with keratoconjunctivitis sicca, pleurisy, and secretory otitis media, is presented. These unusual complications are very similar to the adverse reactions to the beta-adrenergic blocking agent, practolol, which is known to cause sclerosing peritonitis. This similarity suggests an etiological association between the sclerosing peritonitis of this case and that caused by practolol. The sclerosing peritonitis of this case may be considered to occur as part of some systemic disorder, and thus casts doubt on the hitherto accepted hypothesis that sclerosing peritonitis results from infectious peritonitis.

Humans↗
PubMed1993

Induction of a 60-kDa heat shock protein in rat pancreas by water-immersion stress.

1. In this study, expression of a 60-kDa heat shock protein in rat pancreas was investigated before and after water-immersion stress, which has been known as an exacerbation factor of caerulein-induced pancreatitis in rats, by Western blot. 2. A 60-kDa heat shock protein increased after water-immersion stress in both soluble and insoluble fractions of the pancreas. 3. Serum amylase level and pancreas weight did not increase after water-immersion. 4. No pathologic alteration was observed in the pancreas after water-immersion.

Animals↗