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Biomedical subjects

O N Shcheglovitova

Publications and source records attributed to O N Shcheglovitova.

At least 19 recordsLinked to original sources
PubMed2003

Cow milk angiogenin induces cytokine production in human blood leukocytes.

Angiogenin isolated from cow milk induces the production of cytokines IL-1beta, IL-6, and TNF-alpha in human leukocytes; the level of production of each cytokine depends on the concentration of the preparation, and the dynamics of production depends on the time from the beginning of induction. Simultaneous treatment with angiogenin and phytohemagglutinin had an additive effect on the production of cytokines, the time of this effect manifestation being individual for each cytokine.

Angiogenesis Inducing Agents↗
PubMed1998

[Interferon-inducing action of polysaccharide-containing biopolymers from ginseng root and cell culture].

Induction of interferon (IF) and tumor necrosis factor (TNF) under the action of two polysaccharide preparations of ginseng i.e. panaxan-1 (from ginseng root) and panaxan-2 (from ginseng cell culture) was studied. Both the preparations induced production of TNF and IF in human leukocytes. By its properties and the typing results the induced IF proved to be gamma-IF. The preparation from the ginseng cell culture in the doses used had a higher IF inducing activity which could be explained by the difference in the polysaccharide composition of the preparations.

Biopolymers↗
PubMed1989

[Effect of splenic cells sensitized with staphylococcal enterotoxin A on the metastasis of Lewis lung carcinoma in mice].

The effect of intact mouse spleen cells sensitized in vitro with staphylococcus aureus enterotoxin A (SEA) on spreading of mouse Lewis carcinoma was studied. A significant decrease in a number of metastases in the lungs and in the weight of the lungs was observed after multiple intrapulmonary inoculation of spleen cells treated with SEA for 6 hours. The effect was less marked after inoculation of the sensitized cells intraperitoneally or into the femoral muscle of the leg affected with the tumour. After multiple inoculations of the sensitized cells, the spleen cells of the treated animals develop the state of interferon hyporeactivity to SEA but not to PHA or NDV.

Animals↗
PubMed1989

[Interferon production by primary culture of human endothelial cells].

Endothelial cells of human umbilical vein are capable of producing interferon upon induction with Newcastle disease virus, influenza virus, and poly I: poly C, but not staphylococcal enterotoxin A. All the interferons produced belonged to the alpha-type. After treatment with influenza virus the endothelial cells produce two subtypes of alpha-interferon: acid-labile and acid-stable.

Cells, Cultured↗
PubMed1989

[Effect of staphylococcal enterotoxin A on the development of Lewis lung carcinoma in mice].

The effect of Staphylococcus aureus enterotoxin A (SEA) was studied for its effect on the development of the Lewis carcinoma in mice. It was shown that administration of SEA immediately after the appearance of the primary node in mice after transplantation of tumour cells led to insignificant inhibition of the node growth and a slight decrease of tumour metastasizing into the lungs. Inoculation of mice after the appearance of the primary node with 1/microgram of SEA 5 times a week significantly increased their survival rate. The lack of the marked effect of SEA appears to be associated with the disturbance of the immune interferon system functioning in tumour-bearing mice, since the production of serum interferon induced by SEA in mice with tumours was considerably lower than in the intact ones.

Animals↗
PubMed1987

[Non-specific antitumoral activity of staphylococcal enterotoxins].

St. aureus enterotoxins A and B possess an antitumour effect. After intraperitoneal inoculation they decrease the size and in some cases prevent the development of the human hypernephroma in the cheek pouch of golden hamsters. The effect of enterotoxins may possibly consist in inducing the production of endogenous immune interferon which activates the host immune system and enhances the rejection of heterologous tumour cells.

Animals↗
PubMed1987

[Preparation and properties of the staphylococcal toxin causing the toxic shock syndrome].

A method is proposed for isolation and purification of the staphylococcal toxin causing the toxic shock syndrome (TSS). The method includes three steps: aggregation of protein from the cultural filtrate of Staphylococcus aureus strain 1169 in the presence of 0.025% sodium hexametaphosphate at pH 3.0; gel filtration of the concentrated material on the sephadex G75; ion-exchange chromatography on DEAE 32 cellulose. The proposed method permits to obtain the purified biologically active preparation of toxin with the yield about 40%. The obtained preparations are homogeneous in polyacrylamide electrophoresis and as analyzed by immunochemical methods. The mol mass of the isolated protein is 24 kD, it is not immunologically identical to staphylococcal toxins A-D and is lethal for New Zealand white rabbits and chinchilla rabbits. Interferon inducing activity of the protein is identical to the one of staphylococcal enterotoxin type A.

Animals↗
PubMed1979

Comparative study on the anticellular and antiviral effects of interferon and the haemopoietic stem cell inhibition factor.

Pretreatment with crude interferon preparations obtained from suspension cultures of bone marrow, spleen and thymus cells or from mouse L-cell cultures or with mouse serum interferon preparations did not change the colony-forming activity of bone marrow cells on syngeneic transplantation to lethally irradiated mice. Preparations of L-cell culture interferon, dialysed and purified by carboxymethyl-Sephadex (G-25) column chromatography, showed an inhibitory effect on exogenous colony formation by bone marrow cells. The results suggested the presence in crude interferon preparations of a substance either inhibiting the anticellular effect of interferon or stimulating colony formation. The factor produced by thymus cells following their treatment with antilymphocyte serum inhibited colony formation by bone marrow cells and, unlike interferon, possessed no antiviral activity when tested in cell cultures.

Animals↗
PubMed1977

The course of influrenza infection in mice with graft-versus-host reaction.

The course of influenza infection in mice with a developed graft-versus-host reaction (GVHR) was changed. Due to disturbances in the inflammatory process the pneumonia was delayed and less marked. Consequently, the infected mice died later than controls. Influenza virus reproduction in the lungs was more intensive and its persistence more prolonged. Interferon production in the lungs of mice with GVHR was similar to that in the controls.

Animals↗
PubMed1976

[Suppression of interferon synthesis during the "graft-versus-host" reaction in F1(CBAXC57BL/6) mice].

The development of the graft-versus-host reaction (GVHR) in the F1(1CBA X C57BL/6 hybrid mice after the transplantation of spleen cells from the C57BL/6 parent donor resulted in a strong inhibition of the serum interferon production induced by the intraperitoneal injection of the Newcastle disease virus. In vitro with the mouse bone marrow cells during the development of the GVHR the interferon response was first reduced and then disappeared completely. The described phenomenon could therefore serve as an index of the development of the GVHR.

Animals↗
PubMed1975

[Bone marrow interferon].

The nucleated cells of the bone marrow of mouse, rat, guinea pig, chick, cattle and humans proved to be capable of producing interferon in vitro following induction with the Newcastle disease virus. The production of interferon by these cells was characterized by high stability. The bone marrow interferon was not inferior in its activity to the corresponding interferon prepared with the blood leukocytes or splenic cells.

Animals↗
PubMedDate not supplied

[New data on acid-labile alpha-interferon].

Reviews the information on the new concepts about the composition and properties of abnormal alpha-interferon-alpha-acid-labile interferon (alpha-ALI) detected in the blood of patients with AIDS, autoimmune, and other diseases. alpha-ALI includes alpha- and gamma-interferon which is responsible for acid lability. Increased content of serum alpha-ALI and its acid lability are poor prognostic signs in AIDS. A decrease in these values, concomitant with clinical improvement in some autoimmune diseases, indicates involvement of alpha-ALI in the pathogenesis of these diseases. Activating effect of gamma-interferon in vitro is demonstrated on different models, including HIV infection of human vascular endotheliocytes. The effect of alpha-ALI in patients is apparently determined by the ratio of its components alpha- and gamma-interferons.

Acids↗
PubMedDate not supplied

[The production of inflammation mediators by mouse peritoneal cells under conditions of combined exposure to staphylococcal enterotoxin and lipopolysaccharide].

The influence of staphylococcal enterotoxin of type A (SEA) and enterobacterial lipopolysaccharide (LPS) on the production of tumor necrosis factor alpha (TNF alpha), gamma-interferon and active forms of oxygen by mouse peritoneal cells was studied. Both SEA and LPS, when injected to animals, produced stimulating influence on the oxygen metabolism of phagocytizing cells. The highest toxic doses of LPS induced the maximal generation of oxygen radicals. Under the conditions of the development of lethal toxic shock, i.e. after the combined injection of SEA and LPS, the synergic activation of oxygen metabolism was observed, which was also manifested by the pronounced production of TNF alpha and the increased synthesis of gamma-interferon.

Animals↗