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Biomedical subjects

O Ortolani

Publications and source records attributed to O Ortolani.

27 records · Page 2Linked to original sources
PubMed1980

[Preparation of a polyvalent antiserum specific for human muscle proteins. I. Muscle extraction: a macromethod and a micromethod].

Protein metabolism may be upset dramatically by several pathological condition such as starvation, malnutrition, surgical stress. Serum proteins have been investigated in many works but very few studies exist about muscle and organ proteins. This article describes two methods for extraction of muscle water soluble proteins: a macromethod that uses some hundred grams of tissue, and a micromethod that starts from a little muscle biopsia. Extracts have been tested for protein content by electrophoresis on cellulose acetate and isoelectrofocusing on polyacrylamide gel and have shown a rich variety of protein fractions.

Animals↗
PubMed1980

[Preparation of a polyvalent antiserum specific for human muscle proteins. II. Animal immunization and purification of the antiserum].

A new preparation technique to raise rabbit antisera against human water-soluble muscle proteins is presented. Immunization, blood collection and further purification of antibody fraction in serum are described in detail. The present method is fast and easy to perform; chromatography and dialysis are not required and antisera so produced lend themselves to immunoelectrophoretic evaluation of human water-soluble muscle proteins.

Animals↗
PubMed1979

CSF electrophoresis: an adaptation using cellogel RS for identification of protein banding.

A CSF electrophoresis on a particular gelatinized cellulose acetate (Cellogel RS) has been tested. The method allows the routine separation of many protein fractions in concentrated CSF in a very brief time and affords the recognition and quantification of oligoclonal banding, that is of extreme importance in the diagnosis of some neuropathies as multiple sclerosis.

Cerebrospinal Fluid Proteins↗
PubMed1979

An improved immunoelectrophoretic methodology for the evaluation of protein caloric malnutrition.

An improved technique of two dimensional immunoelectrophoresis of serum proteins on gelatinized cellulose acetate for routine evaluation of protein nutritional state is described. The method, which allows the estimation of more than 30 protein fractions, proves extremely useful in monitoring the effectiveness of total parenteral nutrition and protein metabolic conditions. Sera samples from healthy subjects and cachectical neoplastic patients have been examined and preliminary results are reported.

Beta-Globulins↗