Biomedical subjects
Publications and source records attributed to O Prat.
Urinary bladder rupture should be suspected in elderly patients with an acute abdomen. Because symptoms are often atypical among elderly patients, this condition is often undetected. Early diagnosis and treatment could help reduce the mortality rate associated with this condition.
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We report here a new detection method for DNA hybrids on dot blots. The process utilizes DNA or oligonucleotide probes labeled with biotin, followed by recognition with a conjugate of streptavidin and europium cryptate, a time-resolved fluorescent label. Unlike the other lanthanide chelates, this label is an organic molecule embedding a europium ion into an intramolecular cavity. This structure has a better stability in diluted assay media, a good sensitivity even on solid support, and an elevated fluorescence lifetime which allows elimination of most of the background generated by other species present in the assay medium. This procedure is quantitative and detects down to 2 amol of a model DNA, which is similar to other nonisotopic (especially colorimetric) methods. The main advantages of this method are easy automation, quantitation, and rapidity of measurements.
The influence of increased bladder pressure on ureteral peristalsis is described in the literature. All experiments were done on anesthetized animals and vesical contractions were electrically induced. We present an experiment on awake sheep. The animals voided spontaneously while a recording of the peristaltic pressure of the lower ureter was recorded. A rise in the peristaltic pressure in both ureters was observed during micturition. We suggest that the rise in ureteral pressure participates in the antireflux mechanism.
A sixteen day old infant developed candiduria after surgery on a single functioning, hydronephrotic kidney with ureteropelvic junction stenosis. Masses of candida albicans caused obstruction of the ureter with acute anuria. Endoscopic relief of the obstruction together with aggressive antifungal therapy led to irradication of the fungal infection. This case history emphasizes the fact that candida infection in early childhood should be evaluated carefully. Unfortunately no guidelines are yet available for the indications and the preferred mode of treatment, and length of therapy of infantile renal candidiasis.
We report a case of rapid spread of a stable ureteral tumor following retrograde brush biopsy. This unusual complication should be considered when planning the management of patients with suspected ureteral carcinoma.