PubMed HealthSearch

Biomedical subjects

O Robinson

Publications and source records attributed to O Robinson.

4 recordsLinked to original sources

Partial nucleotide sequence of the enzyme pyruvate, orthophosphate dikinase of Entamoeba histolytica HM1:IMSS.

We isolated a clone from a lambda gt11 cDNA library of E. histolytica, subcloned its insert in the vector pTZ18R and sequenced it by the method of Sanger. Sequence comparison with Genbank and of the corresponding amino acid sequence with the NBRF/PIR data bank using the FastA program, showed homologies between 54.9 and 58.5% for the nucleotides and between 48.6 and 49.4% for the amino acids with pyruvate, orthophosphate dikinases from maize, Flaveria trinervia and Bacteroides symbiosus. The sequence obtained for E. histolytica represents about 20% of the complete gene or protein sequence.

Amino Acid Sequence

NCTR computer systems designed for toxicological experimentation. II. Experiment start-up system.

The Experiment Start-Up System (ESS) developed and implemented at the National Center for Toxicological Research constructs models from information contained in experimental protocols. These models may then be used to project resource requirements and related schedules. Each experimental model provides information from which management can determine the raw resources such as quantity of food, water, bedding, cages, animals (sex, strain, species), chemical, facilities, and manpower needed for conducting the experiment. Based upon this information, management can determine if the required resources are available and determine the most feasible "start" date for the new experiment. In addition, the information provided by the experimental model assures the Principal Investigator that the protocol requirements are thoroughly understood by participating technical support groups and that quality control elements of the ESS and other integrated systems will provide a means for accurately monitoring the experiment.

Computers