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Biomedical subjects

O Schulz

Publications and source records attributed to O Schulz.

At least 37 records · Page 2Linked to original sources
PubMed1996

Riboflavin can decrease the nephrotoxic effect of chromate in young and adult rats.

The influence of 5 mg vitamin B2/100 g b.wt. (B2, riboflavin) on the nephrotoxic effect of 1 or 2 mg Na2Cr2O7/100 g b.wt. (Cr) was investigated in 55- and 10-day-old rats, respectively. Nephrotoxic effect was evaluated by the determination of urinary volume and protein excretion as well as the concentration of blood urea nitrogen (BUN). The concomitant administration of Cr and B2 only in 55-day-old rats increased the nephrotoxicity shown by enhanced proteinuria and BUN. B2, administered 3 h after Cr, was able to diminish Cr nephrotoxicity significantly in 55- and 10-day-old rats. The effect of B2 on Cr nephrotoxicity could be interpreted not by the stimulatory effect of B2 on GSSG reductase, which was abolished by Cr; but by its antioxidant effect.

Age Factors↗
PubMed1995

Der p I, a major allergen of the house dust mite, proteolytically cleaves the low-affinity receptor for human IgE (CD23).

The nature of the proteases that cleave CD23 in vivo is of considerable interest, but remains unknown. Here, we demonstrate that Der p I, a major allergen of the house dust mite Dermatophagoides pteronyssinus, cleaves CD23 from the surface of cultured human B cells (RPMI 8866 B cell line). The cleavage of the receptor from the B cell surface was associated with a parallel increase in soluble CD23 (sCD23) in the culture supernatant. Furthermore, the proteolytic effect of Der p I was specific for CD23, since none of the other B cell markers tested (CD20, HLA-DR, CD71 and CD49d) were affected. Labeled antibody experiments and protease inhibition assays clearly demonstrate that Der p I is a cysteine protease that directly cleaves a 25-kDa fragment of CD23. These data suggest that the cysteine protease Der p I, in addition to being highly immunogenic, may up-regulate IgE synthesis by virtue of its ability to cleave CD23.

Allergens↗
PubMed1995

Two strategies to prepare neural cortical cytoskeleton components for the generation of monoclonal antibodies.

Like most other cells, neurons possess a spectrin/actin based network closely associated with the inner side of the cell membrane, the cortical cytoskeleton. This structure serves many diverse functions during axonal outgrowth. In the growth cone, the cortical cytoskeleton is involved in surface shaping, modulation of integral membrane proteins, and signal transduction. We developed two strategies to prepare material enriched for neural cortical cytoskeleton. The first strategy combined the isolation of a membrane/cortical cytoskeleton fraction by density gradient centrifugation with an enzymatic degradation of cell surface proteins. The second strategy is based on the attachment and crosslinking of single cells to beads, allowing for the removal of the cell contents by cell disruption; only membrane/cortical cytoskeleton patches are retained on the beads. Both strategies made use of the intimate association of the cortical cytoskeleton with the cell membrane, permitting the removal of cytoplasm, organelles and cytoplasmic cytoskeleton while retaining the cortical cytoskeleton. Monoclonal antibodies generated using both preparations as immunization material were screened for recognition of intracellular structures in axons and growth cones of retinal ganglion cells in culture. A quantitative specification of the antibodies is presented and six antibodies are characterized in immunolabelings and Western blot analysis.

Animals↗
PubMed1991

[Interrelationships between methylmercury exposure and selenium supplementation in cockerels using selected laboratory diagnostic parameters and toxicological parameters of residues].

A feed loading experiment was applied in 2 phases to 45 young cocks over 12 weeks, using 1,2-N,N-bis(methylmercury)-p-toluolsulphamide-dressed wheat (50% of base ration). Investigations were conducted to study the effects of selenium supplementation (0,2 mg Se as sodium selenite/l drinking water) on biochemical and hematological parameters (calcium, phosphorus, total protein, albumin, creatinine, urea, activity of alkaline phosphatase, hematocrit, hemoglobin, leucocyte count) as well as on parameters relating to toxicological residues (selenium and mercury levels in liver, musculature and kidneys). Statistically secured differences were found to exist between the experimental groups with regard to selenium and mercury in the liver and mercury concentrations in kidneys. Possible interrelationships were discussed.

Animals↗
PubMed1989

[Experimental laboratory diagnostic and residual toxicological studies in young cocks after feeding mercury-disinfected seed with and without selenium supplementation].

A feed loading experiment was applied in 2 phases to 45 young cocks over 12 weeks, using 1.2 (N, N-bis/methylmercury/-p-toluolsulphamide)-dressed wheat (50% of base ration). The experimental animals were White-Leghorn laying hybrids. Investigations were conducted to study the effects of exclusive exposure to mercury and those of mercury with addition of 0.2 mg of sodium selenite/l drinking water on biochemical parameters (calcium, phosphorus, total protein, albumin, creatinine, urea, activity of alkaline phosphatase, hematocrit, hemoglobin, and leucocyte count) as well as on parameters relating to toxicological residues (selenium and mercury levels in liver, musculature, and kidneys). Statistically secured differences were found to exist between the experimental groups with regard to selenium and mercury in the liver and mercury concentrations in kidneys. These data have shown that the problem of residualisation cannot be solved by selenium supplementation in parallel to methylmercury loading. The results recorded are likely to confirm the need for a general ban on feeding mercury-dressed seed.

Animal Feed↗
PubMed1976

[Artificial intranasal bacterial invasion in the newborn calf. 2. Artificial bacterial invasion using gram-positive and gram-negative strains].

A micrococcal and a diplocaccal strain isolated from the nasal space of a clinically intact nursed calf were used for artificial bacterial invasion in the first phase of the experiment. Application of bacterial suspension prepared from those strains had no effect upon the rise of coli counts in the nasal secretion of nursed calves during their first days of age nor upon the morbidity or mortality of all 677 test animals in comparison to 665 controls. Therefore, an avirulent E.-coli strain was used in subsequent bacterial invasion experiments. The strain was retrievable up to the seventh day of age, the count having been about 10(5) bacteria per gram nasal secretion. Application of a bacterial suspension prepared from that E.-coli strain did not reduce morbidity and mortality among 820 test animals that were compared to 809 controls. Results are discussed in this paper with reference to literature.

Animals↗
PubMed1975

[Metabolism and hematology studies of intensively raised fattened lambs under various feeding and management conditions. 1. The effect of various feeding methods on the blood picture, serum mineral and bilirubin concentration, and vitamin A concentration in the liver].

Calcium, phosphorus, magnesium and bilirubin content, blood picture and liver vitamin A were determined in six groups of lambs fattened on different rations, in comparison with a control group. The results are presented in graphs and tables. There was no clear difference between the various groups, in the criteria examined.

Animal Feed↗
PubMed1975

[Metabolism and hematology studies of intensively raised fattened lambs under various feeding and management conditions. 2. The effect of various management methods on the blood picture, serum mineral and bilirubin concentration and vitamin A concentration in the liver].

Calcium, phosphorus, magnesium and bilirubin content, blood picture and liver vitamin A were studied in four groups of intensively fattened lambs kept under different conditions of management, in comparison with a control group. Results are shown in graphs and tables, Group-specific differences were found in some of the parameters examined: - Ca, P, erythrocytes, leucocytes, vitamin A.

Animal Feed↗
PubMed1975

[Metabolism and hematology studies of intensively raised fattened lambs under various feeding and management conditions. 3. The effect of weaning age under various management conditions on the blood picture, serum mineral and bilirubin concentration and vitamin A concentration in the liver].

Calcium, phosphorus, magnesium and bilirubin, blood picture and liver vitamin A were studied in six groups of lambs weaned at various ages and fattened under four types of management, in comparison with a control group. Results are shown in graphs and tables, with a comparison with conventionally fattened lambs. Age at weaning had no demonstrable effect on the parameters investigated.

Age Factors↗