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Biomedical subjects

O Shimada

Publications and source records attributed to O Shimada.

43 records · Page 3Linked to original sources
PubMed1979

Lipid peroxidation and its inhibition by tinoridine, II. Ascorbic acid-induced lipid peroxidation of rat liver mitochondria.

Incubation of rat liver mitochondrial suspension with ascorbic acid and Fe2+ resulted in the formation of malondialdehyde and a decrease in the turbidity of the suspension. The maximum amount of malondialdehyde formed during the peroxidation reaction was estimated to be 1 mol per approximately 6 mol of mitochondrial phospholipids. Tinoridine and alpha-tocopherol at the concentration of 5 micron and 1 mM, respectively, completely inhibited the peroxidative disintegration of mitochondria. From the relationship between the concentration of tinoridine and the amount of malondialdehyde formed, it was demonstrated that 1 mol of tinoridine prevents the formation of about 6 mol of malondialdehyde. These findings suggest that there is a limit in the chain reaction of the lipid peroxidation of mitochondria and that the limit is the membrane sphere which is capable of releasing 6 molecules of malondialdehyde and contains about 36 molecules of the constitutive phospholipids.

Animals↗
PubMedDate not supplied

Effect of nizofenone on experimental head trauma in mice.

The therapeutic effect of nizofenone, a cerebroprotective drug, on experimental traumatic head injury was investigated in mice. The animals under halothane anaesthesia were subjected to a severe concussive head trauma and their neurological status was assessed by a string grip-test for 6 consecutive days. The neurological function of the control groups fell markedly 1 hr after trauma and slowly recovered day by day, but some animals failed to restore to the normal level, even on the 6th day. The post-treatment of the head-injured mice with nizofenone (3 mg/kg/day, i.p.) significantly improved the neurological function. The neurological improvement by nizofenone was dose-dependent and a significant amelioration was observed at 0.3 mg/kg/day. Methylprednisolone (30 mg/kg/day, i.v.) did not exert any significant neurological improvement in this therapeutic, experimental condition. In this study, nizofenone treatment was found to beneficially influence the recovery of the neurological function, maybe by reducing the progress of brain injury following head trauma.

Animals↗