Biomedical subjects

O Toledano

Publications and source records attributed to O Toledano.

4 recordsLinked to original sources

PubMed1997

Formation of Surface Active Gelatin by Covalent Attachment of Hydrophobic Chains

Surface active gelatin was formed by covalent attachment of hydrophobic groups to gelatin molecules. The modification was carried out at various degrees of attachment and with various chain lengths. These modified gelatins (MGs) were synthesized in dry DMSO by a simple and rapid method. The new method leads to high yields and allows high degrees of modification. The MGs, which have various hydrophobicities, have better surface activity than the native gelatin, as determined by surface tension reduction. The surface tension reduction is correlated to the hydrophobicity of the modified molecule, which was determined by a fluorescent probe. It appears that both the increase in the number of the hydrophobic groups and the increase in the chain lengths lead to decreased surface tension. Copyright 1997 Academic Press. Copyright 1997Academic Press

Journal Article↗

PubMed1996

Solubilization in Colloidal Immunoclusters

Micelle-like clusters of antibody molecules were prepared by covalent attachment of various hydrophobic groups to the protein molecules. These colloidal clusters were capable of solubilizing two hydrophobic probes, while the solubilizate:solubilizer molar ratio was dependent on the chain length of the hydrophobic groups, the degree of modification, and hence, on the size of the colloidal clusters. By using a fluorescent solubilizate, it was demonstrated that the immunoclusters may have a specific recognition ability.

Journal Article↗

PubMed1992

Fine-needle aspiration and cytologic findings of surgical scar lesions in women with breast cancer.

Benign and/or malignant lesions may occur in surgical scars after mastectomy or lumpectomy (SML) in patients with breast cancer (BC). Early diagnosis of these lesions is essential for both therapeutic and prognostic evaluation. The diagnostic value of fine-needle aspiration (FNA) was determined for these scar lesions. The findings of cytologic and histologic specimens obtained from the same lesion of SML in 83 women with BC were correlated. Twenty-five FNA yielded only acellular specimens. Of the FNA done by the cytopathologist, only 6.2% were not representative. However, 45% of those done by less experienced clinicians were not representative. Representative FNA were obtained from 58 of the women who took part in the study. Based on the histologic diagnosis, 38 patients had malignant scar lesions (MSL), and 20 had benign scar lesions (BSL). In one patient of the 38 with MSL, cytologic examination did not show that the malignant lesion; in four women, the tumor was suspected cytologically; and in the remaining 33, the cytologic findings were consistent with malignancy. In 18 of the 20 patients with BSL, cytologic findings were reported as benign and in the other two, as inconclusive. The sensitivity, specificity, and positive and negative predictive values for the cytologic findings were 97.4%, 100%, 100%, and 94.7%, respectively. The diagnostic accuracy of FNA cytology was 98.2%. No complications followed the procedure. It was concluded that FNA cytologic examination of lesions in SML is a simple, safe, highly accurate, and cost-effective method to distinguish malignant from benign lesions in women with BC. Lesions in SML should be explored routinely by FNA, rather than by the traditional biopsy, provided the FNA is done by an experienced operator.

Adult↗

PubMed1991

Fine-needle aspiration cytology of abdominal wall scar lesions for diagnosing recurrent colorectal cancer.

Nodules in the abdominal wall scar after resection of colorectal cancer may represent nonmalignant or malignant lesions. We report clinical and fine-needle aspiration (FNA) cytologic findings in five patients with nodules suspected of being malignant. All patients had had adenocarcinoma, four of the colon and one of the rectum. Postoperative abdominal wall irradiation had been administered to three patients. The median time from surgical removal of the cancer to FNA of scar nodules was 27 months. Three patients had malignant and two patients had nonmalignant FNA cytology, histologically confirmed at surgical biopsy of scar nodules. The malignant histologic cell type demonstrated by FNA cytology of the scar lesions was identical to that exhibited by histology. The survival of patients with positive cytology and histology ranged between 9 and 43 months; survival of patients with negative cytology and histology was between 53 and 138 months. We conclude that FNA cytology is a simple, sensitive, and specific procedure to evaluate patients with scar nodules appearing after resection of colorectal cancer. This procedure may safely replace surgical biopsy in the initial evaluation of scar nodules.

Abdominal Muscles↗