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O V Chistiakova

Publications and source records attributed to O V Chistiakova.

At least 19 recordsLinked to original sources
PubMed2006

[Evaluation of the diagnostic significance of cytologic studies in pancreatic tumors].

The study was undertaken to specify cytomorphological criteria for prostatic duct adenocarcinoma (PDAC), by assessing fine-needle aspiration biopsy (FNAB) specimens. Histologically verified cases were selected and the archival cytological material that constituted Leishman stained smears obtained by percutaneous FNAB under ultrasound guidance from 72 patients with PDAC (n = 50) or ductal hyperplasia (DP) (n = 22) was revised. For statistical analysis, the authors selected the following cytological signs of malignancy: anisonucleosis, increased nuclei, hyperchromia, uneven chromatin distribution, enucleation, uneven nuclear outline, apocytes; increased nucleoli, nucleolar polymorphism, multiple nucleoli, increased cells, anisocytosis (polymorphism of the size and shape of a cell, mitoses, piled nuclei, papillary structures, slightly glandular structures. The statistical analysis identified three most important cytological criteria for PDAC: anisocytosis, anisonucleosis, and piled nuclei. Keeping in mind all three signs at once increases the probability of detecting of PDAC up with 90%. The detection of only anisocytosis in the cytological specimen showed a 40% probability of the presence of PDAC. Three additional cytological signs of PDAC were also identified: increased nuclei, uneven nuclear outline, and slightly adhesive structures. The probability of PDAC was 100% if the specimen contained three most significant signs and any of the additional signs. By taking into account the indicators of diagnostic efficiency (DE), the best criteria were as follows: anisonucleosis, uneven nuclear outline, anisocytosis. The optimal indicators of DE for anisonucleosis were 95.83% diagnostic sensitivity (DSen), 95.83% diagnostic specificity (DSp), and 95.83% DE. When two indicators (anisonucleosis and uneven nuclear outline) were simultaneously used, DSen was 93.42; DSp, 98.53% (the highest); DE, 95.83%. Thus, the following cytological signs are defined as important for differentiation of PH and PDAC: anisocytosis, anisonucleosis, piled nuclei, increased nuclei, uneven nuclear outline, slightly adhesive structures.

Adenocarcinoma↗
PubMed1999

[Formalized cytological report in oncology].

A formalised report (FR) offers a set of standard phrases to be used to state (1) whether findings of a cytologic study contain new information; (2) the degree of certainty in understanding the significance of morphological forms of available international classifications of a pathology said morphological evidence is compatible with. The use of that FR provides a means of evaluating reported cytologic conclusions, and comparing them with relevant postmortem diagnosis. This makes it possible to ascertain with greater or less certainty, with which nosological forms a doctor deals. It is also a means of inspection over the way materials are sampled and processed at a particular cytological laboratory as well as evaluation of its general standards. The main advantage of introduction of FR is that it is unambiguous and can be computer processed.

Cell Biology↗
PubMed1999

[Feasibilities of cytological diagnosis in the static telepathology mode].

Results of cytological diagnosis in the static telepathology mode are analyzed. Three expert cytologists made the diagnosis on the basis of images of cytological preparations obtained from 94 patients with tumors of soft tissues and bones. A total of 113 diagnoses were correct: benign or malignant type of the tumor and its histological type were identified. In general, a total of 152 (88.9%) diagnoses were correct as regards their significance for treatment strategy. Errors classified as groups 3 and 4 were the most significant (erroneous assessment of tumor type--benign or malignant--in 6.4% cases). Analysis of discrepancies between cytological and pathoanatomical diagnoses and data of routine optic microscopy of similar cytological preparations of the same material (bone and soft tissue tumors) showed the same value: 6.8% of cytological and pathoanatomical diagnoses were at disagreement (groups 3 and 4 of errors). Therefore, telepathology can be resorted to for consultations and discussion of obscure cases. It is particularly important for a morphologist to discuss complex cases by means of telepathology at a specialized institution, distant from him or her.

Cytodiagnosis↗
PubMed1991

[Comparative evaluation of gastro-intestinal tract tumors on the basis of data from determination of DNA content using flow cytometry].

Morphologic and cytophotometric characteristics of tumours and tumour-like lesions of the stomach, colon and rectum from 267 patients were compared in the biopsy material. There was a diploid DNA distribution in the adenomas and tumour-like lesions of colon and rectum. The correlation between the proliferative activity and the epithelial dysplasia degree was noted in adenomas; aneuploid cells were found in 5 adenomas. 53% of gastric carcinoma and 64% of colon and rectum carcinomas were aneuploid (the difference is not significant). Poorly differentiated adenocarcinoma much more frequently (the difference is significant) was found in the aneuploid tumours, well differentiated adenocarcinoma and signet cell carcinoma- in the diploid tumours. DNA index (1.09-1.50) of the aneuploid cells of gastric carcinoma was in 42% cases in the near diploid--triploid region while in colon and rectum carcinoma (60.5%) it was in the triploid--tetraploid region. The type of DNA distribution, particularly in the diploid tumours, should be controlled cytologically.

Adenocarcinoma↗
PubMed1990

[Ultrasonographically-controlled percutaneous puncture biopsy in the diagnosis of cancer of the pancreas].

The article deals with the method and results of ultrasonically-guided fine needle percutaneous biopsy performed in 45 patients for differential diagnosis of cancer of the pancreas. Thirty-four patients had tumors of the pancreas, 10--chronic indurative pancreatitis, one patient had a cyst. The diagnosis was verified morphologically in 34 patients: in 25 of 31 patients with primary tumors of the pancreas and in 9 of 11 patients with chronic pancreatitis and a cyst. The method is safe, highly informative and allows the character and nature of the pancreatic tumor to be determined precisely and the tactics of special treatment to be thus elaborated.

Biopsy, Needle↗
PubMed1989

[Flow cytometry of DNA in research on kidney tumors].

The DNA content was studied in 22 patients (including 10 children) with renal tumours. Renal clear cell carcinomas were mainly diploid (5 of 6 cases); one clear cell and other variants of renal cell carcinoma were aneuploid. There was a correlation between the degree of tumour cell anaplasia and ploidy of renal cell carcinomas: all diploid carcinomas were of I and II degree of anaplasia, aneuploid carcinomas, except one case, were of degree III of anaplasia. 5 out of 9 nephroblastomas were diploid, 4, aneuploid. The distinctive features of nephroblastoma were pronounced proliferative activity of tumour cells and a low DNA index of the aneuploid cell line which in all cases was localized in the vicinity of the diploid region. The remaining tumours (papillary epithelial nephroma, juxtaglomerular cell tumour and malignant schwannoma) were diploid with a relatively low proliferative activity of tumour cells.

Adult↗
PubMed1987

[Possibilities for defining dysplastic changes in the gastric mucosa during cytologic studies of gastric biopsies].

The authors compared the data of cytologic and histologic studies of gastric biopsy material from 95 patients with different degree of epithelial dysplasia revealed histologically. Differentiation impairment and epithelial cell atypia can be revealed in cytologic preparations; basing on these features epithelial dysplasia was diagnosed cytologically in 40 patients (30 of them with adenomas). Cytological and histological diagnosis of epithelial dysplasia did not fully coincide. The most reliable are the cytological features of severe dysplasia, thus the main goal of a morphologic study is achieved. One should not identify the term "dysplasia" in a cytologic study with a term "proliferation". Gastric biopsy material may have the following cytologic characteristics: tegmental-fossa epithelium proliferation, marked proliferation of tegmental-fossa epithelium with atypia, epithelial dysplasia and such epithelial dysplasia when cancer is not ruled out.

Biopsy↗
PubMed1986

[Morphological and cytophotometric characteristics of polyps of the colon and rectum].

Material from 60 patients with colon and rectum polyps as well as "normal" mucous membrane (44 patients) taken at some distance from polyps are studied. DNA content was measured by a flow cytometry method (ICP-II) in parallel to morphological (cytological and histological) examination of biopsies. Analysis of material was performed by groups depending on the epithelial dysplasia degree in the adenomas. Diploid cells dominated in the adenomas. Significant increase of cells in S- and (G2 + M)-phases of cell cycles is noted in the adenomas with a severe epithelial dysplasia as compared to the adenomas with a mild or moderate dysplasia. Aneuploid cells are detected in 5 adenomas, 4 of them morphologically were those with a severe dysplasia including 2 cases in which it was difficult to differentiate between a severe dysplasia and carcinoma although the signs of invasion were absent.

Colonic Polyps↗
PubMed1981

[DNA content of gastric mucosal epithelium in various diseases].

Evaluation of DNA content in superficial epithelial cells of gastric mucosa by gastrobiopsy using the flow-through cytophotometric method revealed some differences in the distribution of DNA values in benign and carcinoma cases. The polymorphism of epithelial cells in benign gastric disorders is accompanied, as a rule, by an increase in the amount of cells in S and G2 + M phases of the cell cycle. In the cases with epithelial cell atypia, a small peak in the hypertetraploid range is often seen and DNA-histograms are identical with those in carcinoma of the stomach. Malignant specimens usually have an increased 3n and 4n DNA level and an obvious third peak at the hypertetraploid level. Different forms of carcinoma (adenocarcinoma and undifferentiated form) have their own distinguishing features in DNA content distribution.

Adenocarcinoma↗