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O V Smirnova

Publications and source records attributed to O V Smirnova.

104 records · Page 6Linked to original sources
PubMedDate not supplied

[Use of trental for correcting the pharmacological effects caused by voltaren and indomethacin].

Treatment of the adjuvant disease in rats by intragastric administration of voltaren or indomethacin (3 mg/kg) was followed by approximately equal anti-inflammatory and analgesic effects on the affected joints. In this case the development of active dystrophic changes in the gastrointestinal tract and kidneys was observed. At combined administration of the antiphlogistics with trental there was noted an enhancement of their therapeutic action on the joints and also a weakening of the ulcerogenic effect and nephrotoxicity that was reflected in the clinical, pathological, morphological and laboratory studies.

Animals↗
PubMedDate not supplied

[Various mechanisms of the participation of the hypophysis and somatotropin in the hormonal regulation of the level of specific estrogen-binding protein in the rat liver].

A study was made of the role of the hypophysis and STH in the regulation of the level of specific estrogen-binding protein (SEBP) and estrogen receptors (ER) in the rat liver, in the expression of the SEPB-determining effect of androgens (A) and the regulating action of estrogens (E) on the SEPB level. The SEPB and ER content was determined by quantitative methods permitting the differential testing of one E-binding protein from another. Hypophysectomy of males caused a decrease in the SEPB and ER levels. STH administration increased the SEPB and ER levels in the liver of the hypophysectomized males but did not influence the level of these proteins in intact animals. Hypophysectomy of intact and ovariectomized females blocked the SEPB-determining action of A even at early time after operation. Estrogens (E) were capable of producing an inhibitory effect on the SEPB level at early time after hypophysectomy of males. The efficacy of E action after hypophysectomy decreased in parallel with a decrease in the ER content in the liver. The administration of STH to hypophysectomized males resulted in an increase in the ER level and a subsequent reduction of SEPB negative reaction to E. It was concluded that hypophysial factors could produce a direct, sensitizing and permissive for sex steroids action on the SEPB level.

Animals↗
PubMedDate not supplied

[Role of protein biosynthetic processes in realizing the action of sex steroids on the level of specific estrogen-binding protein in the rat liver].

The authors have assessed the life-span of special estrogen-binding rat liver protein (SEBP) through inhibition of it by long-term administration of cyclohexymid (C1) and have examined how C1 affects the efficiency with which sex steroids influence the level of SEBP. The level of SEBP synthesis was assessed by the changes in the number of SEBP estradiol (E2)-binding sites. The long-term C1 administration in the dose of 100 micrograms suppressed SEBP synthesis by an average of 82.4% with the half-life of SEBP being about 18 hr. Testosterone propionate (TP) and 5 alpha-dihydrotestosterone, but not E2, were found able to mediate enhanced SEBP levels in the liver of ovariectomized female rats. A single 200 micrograms dose of C1 administered 30 min prior to hormone injection caused significant decreases in the efficiency of SEBP-mediated androgen activities in the liver of ovariectomized female rats, in TP-induced stimulation of the SEBP level in the liver of castrated male rats and in E2 inhibitory effect on the SEBP level in the liver of mature males. It is concluded that de novo protein synthesis is necessary for the early manifestation of all the above mentioned effects of sex hormones on the SEBP level. Based on the data regarding the duration of SEBP life and the rate with which it affects the activity of sex steroids it is supposed that in the early stages of hormonally induced manifestations there are changes in biosynthesis of regulating protein rather than in SEBP itself.

Animals↗
PubMedDate not supplied

[Role of estrogens in the induction and regulation of a specific estrogen-binding protein in the rat liver].

A study was made of the role of estrogens (E) in the induction and regulation of the level of specific estrogen binding protein (SEBP) in the rat liver using a differentiated quantitative method of its determination. It was shown that E could not replace androgens (A) in the primary determination of the SEPB level, neither did they prevent its A-dependent induction. Multiple administration of 0.4 microgram of estradiol (E2) caused a significant decrease in the SEPB level in intact and castrated male rats as well as in ovariectomized females with the A-induced SEPB level. Multiple administration of even 10 micrograms of E2 did not influence the SEPB level in hypophysectomized males. The rate of development of the E2 effect on the SEPB level of the mature male liver increased with the growth of the dose and duration of hormone administration. The inhibitory effect of E2 was also revealed in a single administration of 10 micrograms of the hormone. In that case the effect was observed after a 3-day lag period, and a maximum decrease in the SEPB level occurred in 6 days. The regulatory negative effect of E2 was reversible. Complete regeneration of the initial SEPB content took place 10-12 days after the development of the maximum effect of E2.

Animals↗
PubMedDate not supplied

[Increase in the agglutination activity of rat blood serum following total body irradiation with supralethal doses].

Irradiation of immunized (during the decrease in the immune response, i.e. on the 14th day following the immunization with sheep erythrocytes) and nonimmunized rats leads to a considerable increase in the level of immune and normal antimicrobial antibodies 3 and 24 h following irradiation. Irradiation on the 7th day of the immune response does not cause the increase in the level of antibodies.

Animals↗
PubMedDate not supplied

[Androgen induction of specific estrogen-binding protein of rat liver: relationship between the stage of ontogenesis and various endocrine factors].

A new modification of specific estrogen-binding protein (SEBP) assay in the liver was used to measure the content of this protein at different stages of ontogenesis in male and female rats after administration of testosterone propionate (TP). It was shown that liver capability to form SEBP could be initially programmed by androgens. The crucial period of the SEBP-determining action of androgens in females is not restricted, since potential capability of androgens of primary induction of SEBP may be realized both neonatally and in the course of pubertation. The emergence of SEBP in the liver of males may be the result of natural pre(neo)natal androgenization. Androgens do not play any decisive role in the control of the level of induced SEBP, inasmuch the content of SEBP in the liver of non-pubertal and pubertal males does not appreciably change after TP administration but grows with pubescence. SEBP is capable of long-term existence in the absence of androgens. The presence of the ovaries produces an inhibitory effect on the level of androgen-induced SEBP and the time of its existence after TP discontinuation. Hypophysectomy does not provoke SEBP emergence in pubertal intact and ovariectomized females but prevents its induction with androgens. Adrenal- and thyroectomy produce no influence on the SEBP-programming effect of TP or promote its spontaneous appearance in the same groups of female rats.

Aging↗
PubMedDate not supplied

[Identification of estrogen-binding proteins in the human liver].

Several properties of estradiol (E2)-binding cytosol macromolecules in the liver of females with cholecystitis and of males with hepatic cirrhosis were studied by means of comparatively delicate methods of free- and bound hormone separation. E2-binding macromolecules with Stokes' radius of approximately 5 nm were found in the hepatic samples under study, formed with E2 complexes, similar to estrogen receptors, according to hormonal specificity of affinity and stability. Protein, similar to a specific estrogen-binding protein of the rat and guinea pig liver, was not found in the preparations examined.

Adult↗
PubMedDate not supplied

[The sex dependence of the expression of the particular estrogen-binding protein in the hepatocytes in rat ontogeny].

Formation of dependence on sex of the unusual estrogen-binding protein (UEBP) expression in rat liver during postnatal development was studied using radioligand and immunohistochemical techniques. UEBP was shown to appear in the male and female liver by day 45 to 50 of postnatal life. In females, UEBP remained at a low level until day 75 to 80, corresponding to faint UEBP-specific staining of a single layer of hepatocytes surrounding the central vein. Thereafter UEBP level decreased below the resolving capacity of the method. In males, UEBP content progressively increased during subsequent stages of ontogenesis. This is accompanied by formation of a stable descending centrolobular-periportal gradient of UEBP-positive hepatocytes. High UEBP expression in the male liver was found to result from the programming effect of androgens exerted between days 1-3 and 12 of postnatal development. The formation of hepatic lobuli is not a condition sufficient for switching on the androgen program of UEBP expression. The absence of testicular androgens in males after day 12 of life does not affect the androgen-programmed ontogenetic time course of UEBP expression at high levels. Experimental programming of UEBP expression in hepatocytes of immature females with the use of androgens results in lower UEBP levels than in males. These levels are comparable to those found in male liver at early stages of regeneration (96 h) after partial hepatectomy. It is suggested that androgens determine not only UEBP-producing capacity of hepatocytes but also the system of its control.

Aging↗
PubMedDate not supplied

[Synergism and interference in the combined biological action of ionizing and SHF radiation].

In experiments with 933 (CBA x C57Bl)F1 mice it has been found that with any variant of alternation and sequence of single ionizing and SHF irradiation with lethal doses, the radiation affection caused by gamma quanta is aggravated. Preirradiation with gamma or SHF radiation ameliorates the severity of the affection caused by the subsequent SHF radiation. Parenteral injection of colloidal sulfur 60 min before SHF radiation causes a 2-5-fold increase in the survival rate of exposed and protected mice as compared to unprotected animals.

Animals↗
PubMedDate not supplied

[The assessment of the mental health of children with psychosomatic diseases].

The mental health of children and adolescents who suffered from gastrointestinal diseases due to psychosomatic causes (duodenal ulcer, chronic gastroduodenitis) was examined. Those with the diseases are a group at risk for nervous and mental disorders, and impaired psychophysical adaptation.

Adaptation, Psychological↗