[The use of the microwave oven in the stages of preparing material for histological research].
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Histological Techniques↗
Biomedical subjects
O V Tarnopol'skaia
Publications and source records attributed to O V Tarnopol'skaia.
[Histologic tissue processing in an automated microwave histoprocessor].
A new vacuum histoprocessor has been tested as well as new more effective protocols to work with this processor. The device allows histoprocessing of 50 samples simultaneously of surgery, biopsy or autopsy material during 1-1.5 h. Apart from the standard protocols, new protocols with a much lower (5-10 times) expenditure of the chemicals can be implemented due to combination of microwaves and vacuum. Each protocol was tested repeatedly (20 and more times) with stable good quality of microscopic image.
Automation↗
[A method of preparing sections for express histological diagnosis].
The method of improving quality of histological slides for intrasurgical diagnosis is proposed. The method consists in preliminary microwave processing of the slice of surgical material before putting in into cryostat for freezing and sections preparation. A slice of surgical material was first immersed in 10 ml of physiological solution (pH = 7.2). Then it was exposed to microwave radiation (2.45 GHz, 1 W/cm2) for 1.5 min. After that the slice was put into cryostat and frozen. Microwave processing is done in a home microwave oven or working chamber of microwave histoprocessor. Essential advantage in quality of slides is obtained.
Histocytological Preparation Techniques↗
[Change in adaptability and kinetics of T-lymphocyte adaptation to hypotonia in pathological states of mammals].
The kinetics of adaptation of the volume of T-lymphocytes and their membrane potential in a hypotonic medium is investigated. It is shown to have a quasiperiodic character. The shortest adaptation period T = 2 min characterizes the adaptation of T-lymphocytes test animals, the most complete adaptation of the volume and the membrane potential in hypotonic medium. Upon adaptation their values are only 6% higher than respective values in an isotonic medium. If an aseptic inflammatory process develops in a mammalian organism, the adaptation period increases to T = 2.5 min, while the volume and the membrane potential remains 28% higher than in an isotonic medium. In the course of development of induced cancerogenesis, 10 min after the cells have been placed in a hypotonic medium, their size is equal to that at the point of maximal swelling, while the membrane potential is 20% lower than that in an isotonic medium. The reason for the varying adaptability of T-lymphocytes is, in our opinion, that, as the inflammation develops, the concentration of corticosteroids increases, the concentration of intracellular K+ decreases, the concentration of macromolecules increases and the potential of the plasmatic membrane of the cell rises, thus leading to the slowing down of the process of adaptation and its decreased extent. The lack of regulation of the volume by T-lymphocytes of mammals in the organisms of which cancerogenesis develops, testifies to the fact that, in such a case, cells lose their adaptability to changing external conditions. Therefore, the disappearance of adaptability of T-lymphocytes on the third day of development of cancerogenesis represents an early test of the process.
Adaptation, Physiological↗
[Acceleration of histologic tissue processing and decalcification using a microwave oven].
Feasibility of significant acceleration of histoprocessing by using domestic microwave oven is shown. Two histoprocessing protocols for a single bit and many bits of surgery, biopsy and autopsy materials have been introduced. The design of a special container is described. The histoprocessing period was reduced to 2 hrs. Significant acceleration (10 to 20 times) of decalcification of bone tissue by using domestic microwave oven was achieved. Decalcification took from 3 to 8 hrs in good quality of the microscopic images.
Bone and Bones↗