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Biomedical subjects

O Vejbora

Publications and source records attributed to O Vejbora.

At least 19 recordsLinked to original sources

[The of effect of rolitetracycline on oxygen consumption in a suspension of E. coli].

Authors studied the oxygen demand by E. coli suspensions (serotypes 023 and 026) modifying it with various concentration of Rolitetracyclin, and proceeded by comparison of both serotypes. Time dependence of oxygen demand has been determined using the direct Warburg's manometric method. The obtained results are summerized as following four points: 1. Tested Rolitetracyclin doses caused the decrease in oxygen demand of E. coli suspensions significantly for the majority of time intervals. Rolitetracyclin actioned similarly in both serotypes. 2. Rolitetracyclin 0.5 microgram concentration actioned bacteriostatically on both serotypes, and showed more pronounced action on E. coli 026 serotypes with higher metabolic activity. 3. Rolitetracyclin 5 and 50 micrograms bactericidal concentrations inhibited markedly the oxygen demand in both serotypes. 4. Using this method allowed to determine the difference in metabolic activity of both E. coli serotypes tested.

Escherichia coli

Experimental production of colicin type V of Shigella sonnei unusual in nature.

Col V factor of Fredericq's strain Escherichia coli 1.7a was mobilized for a transfer by the R (Sm, Cm, Tc, Su) factor of Shigella sonnei strain in three component test. The strain E. coli K13 HfrR (a universal indicator for evidence of colicinogeny) and S. sonnei strain became the recipient of both plasmids. S. sonnei, a producer of colicin V has not been found in natural conditions as yet.

Bacteriocin Plasmids

Comparison of direct and indirect migration inhibition tests and skin hypersensitivity to tuberculin (PPD).

The direct macrophage and indirect leukocyte migration inhibition tests were used for the examination of patients with pulmonary tuberculosis and of their contacts. In the direct test at a concentration of 10 microgram PPD/ml medium, a statistically significant difference [p less than or equal to 0.001] was found between the mean values of MI [migration indices] in PPD-negative [MI = 0.98 +/- 0.10] and MI of PPD-positive to patients [MI = 0.60 +/- 0.10]. [PPD-negative = showing no reaction to ID administration of tuberculin, PPD-positive = showing a positive reaction to ID administration of tuberculin]. A statistically significant difference [p less than or equal to 0.05] was also found between the mean values of MI in tuberculin-positive to patients and healthy contacts [MI = 0.80 +/- 0.14]. By means of the indirect test we demonstrated a statistically significant inhibition of migration [p less than or equal to 0.001] in PPD highly positive contacts [MI = 0.76 +/- 0.10]. The results of the direct and indirect migration inhibition tests were found to agree in 66.6%.

Cell Migration Inhibition