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Biomedical subjects

O Vesterberg

Publications and source records attributed to O Vesterberg.

At least 91 records · Page 5Linked to original sources
PubMed1978

Determination of cadmium in urine by graphite-furnace atomic absorption spectroscopy.

A simple procedure for graphite-furnace atomic absorption analysis of cadmium in urine, based on the use of a temperature-controlled heating unit, is described. The temperature of the carbon rod is measured by resistance and controlled by electronic feedback. We studied the influence of various heating times and temperatures of ashing and atomization, as well as suitable diluents. Urine samples were diluted with an equal volume of 0.3 mol/liter HNO3, and 1 to 5 microliter of the mixture was analyzed, with background correction. Signals were evaluated electronically, giving values for integrated peaks as well as for peak heights. The detection limit was 1.2 nmol/liter. The method appears to be accurate and reproducible. The CV averaged 8% for concentrations in the range 20 to 100 nmol/liter. Correlations with a chelation-extraction method was 0.994.

Cadmium↗
PubMed1977

Staining of proteins after isoelectric focusing in gels by new procedures.

Four procedures are described for staining of protein bands after isoelectric focusing in polyacrylamide gels. In one procedure, Coomassie Brilliant Blue R 250 is used whereas in the others the G 250 form is used in an aqueous solution of perchloric acid. By using isoelectric focusing and the new staining procedures it is possible to detect less than 0.2 microng of many proteins. One of the procedures is especially rapid, allowing detection of proteins without destaining. Procedures for preservation of the protein patterns and determination of proteins by scanning in a densitometer are also described.

Evaluation Studies as Topic↗
PubMed1976

Abnormal tissue distribution of lead in amyotrophic lateral sclerosis.

The lead content of cerebrospinal fluid (CSF) was found to be significantly elevated in 12 patients with amyotrophic lateral sclerosis, when compared to 28 control subjects having non-degenerative neurological disorders. The difference could not be explained as being merely secondary to blood-CSF barrier damage. A hypothetical model of the pathogenesis of the disease is advanced and the results are discussed in relation to this model.

Adult↗
PubMed1976

Urinary proteins in occupational exposure to chemicals and in diseases.

Isoelectric focusing in thin-layer polyacrylamide gel is a method with high resolving power, e.g. for urinary proteins. Therefore more information can be obtained with this method than with ordinary electrophoresis. The present version of the method has a high capacity and allows study of up to 25 samples in parallel on each gel. Only a little more than one hour is required for separation. A sensitive staining procedure is used which allows detection of as little as 0.1 mug of protein in each zone. When effects on the kidney are present more than 20 different protein zones can sometimes be separated. Evaluation is made by densitometry as well as by comparison and classification of patterns. Urine samples have been studied from workers with varying exposure to cadmium lead and various chlorinated hydrocarbons, and patients with glomerulonephritis, pyelonephritis, nephrosis and mixed tubular and glomerular diseases. Typical tubular patterns, i.e. as developed in association with cadmium exposure were characterized by elevated excretion of beta1-microglobulin. Other types of kidney malfunction showed different features.

Cadmium Poisoning↗
PubMed1976

Exposure to trichloroethylene II. Metabolites in blood and urine.

Fifteen men were exposed to trichloroethylene (TRI) in three different ways with regard to the concentration of TRI in the air as well as exercise on a bicycle ergometer. The total amount of TRI supplied and taken up by each person was measured. The concentrations of trichloroethanol (TCE) and trichloroacetic acid (TCA) were determined in blood and urine. In spite of large differences in uptake, there were only small differences in the concentration of TCA in blood during the day of exposure. There was a large scatter for the values of TCA in urine within each group. The concentration of TCE in arterial blood increased during exposure. Thereafter the concentrations were almost constant for 2 h and differed among the groups. These results can be interpreted as being due to balanced rates of the formation and elimination of TCE. The levels mentioned were related to the uptake of TRI. The same was found for the rate of excretion of TCE in urine when calculations were made from the morning sample obtained the day after exposure.

Chlorohydrins↗