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Biomedical subjects

O Wolf

Publications and source records attributed to O Wolf.

At least 19 recordsLinked to original sources
PubMed2000

Necrobacillosis in free-living male European bison in Poland.

In the Bialowieza Primeval Forest (Poland) a chronic disease of the external genital organs has been observed in free-living male European bison (Bison bonasus) since 1980. Investigations on this disease started in the late 1980s. The most striking findings are necrotic and ulcerative lesions of the prepuce and penis of bison aged from 6 mo to >10 yr. Histologic examination of tissue samples from the prepuce of six bison (9-mo- to 8-yr-old), and from the penis of two bison (3- and 8-yr-old), were characteristic of necrobacillosis. Masses of slender, Gram-negative, rod-like or filamentous bacteria occurred in necrotic tissue. At the periphery of necrotic tissue filamentous bacteria were often arranged in large clusters and strands that advanced towards healthy tissue. Immunolabeling and electron microscopy also suggest that these organisms are Fusobacterium sp.

Animals↗
PubMed1999

[First experiences using microsurgical techniques for minimally invasive ventral interbody fusion of the lumbar spine (MINI-ALIF)].

Minimal invasive techniques recently have become more important in spine surgery. Ventral approaches for lumbar fusion techniques are known for a long time. Their disadvantages are their size and the associated trauma. Since the early nineties two schools of minimal invasive procedures were developed. On one hand surgeons developed laparascopic methods, on the other hand traditional ventral approaches were minimized and combined with microsurgical techniques, in a way that they offer all advantages of minimal invasive procedures. This technique widely known under the acronym MINI-ALIF, which stands for MINImal invasive Antero-Lumbar Interbody Fusion, was developed for retroperitoneal approaches to the segments L2/3, L3/4, L4/5 and for the transperitoneal approach to the segment L5/S1. Its advantages include reduced technical and personnel effort, minimal traumatized tissue, low blood-loss, short operating times and reduced rates of complications. Additionally the surgeon does not need to be experienced in laparascopic methods. The operation technique is explained and discussed from the neurosurgical point of view.

Humans↗
PubMed1998

[Cystic echinococcosis of the spleen].

Based on the portal infection root, the hydatid disease of the spleen represents a rarity. Because of a lack of prospective studies, spleen-preserving procedures and splenectomies were published as surgical therapy in case reports. In this report, the case of a 72-year-old woman with symptomatic hydatid disease of the spleen will be reported. Surgical and pharmaceutical strategy to minimize the risk of an OPSI syndrome as well as the value of adjuvant therapy with Albendazole will be discussed.

Aged↗
PubMed1994

Endorectal ultrasound and leiomyosarcoma of the rectum.

Leiomyosarcomas of the rectum are uncommon neoplasms accounting for fewer than 1% of all malignancies of the colon and rectum. Approximately 215 cases have been described in the literature. Distinction from leiomyoma is often difficult, but regarding dignity is important. The case reported is that of a 68-year-old man in whom a 2.2-cm rectal mass covered by mucosa was diagnosed by rectoscopy and endorectal ultrasound. After treatment by wide local excision the histological specimen revealed a low-grade, highly differentiated leiomyosarcoma. Due to the lack of a large series of this disease, there is disagreement over the therapeutic strategy. At the moment a selective treatment approach seems to be the most frequently advocated. Large tumors and those extending beyond the rectal wall are treated by radical surgical resection. Leiomyosarcomas less than 2.5 cm in size and confined to the bowel wall can be treated by wide local excision. Endosonography can provide exact assessment of tumor size and expansion and is of great value in selecting the appropriate treatment.

Aged↗
PubMed1992

The traumatic dural sinus injury--a clinical study.

In a period of 13 years 978 cases of severe head injuries were operated on in our clinic. An analysis of the medical reports includes injuries of the superficial dural sinus (39 cases = 4%): among these injuries of the anterior and central part of the superior sagittal sinus (66 per cent), injuries of the transverse sinus (18 per cent), injuries of the posterior part of the superior sagittal sinus (8 per cent), and combined injuries of different dural sinuses (8 per cent). Clinical data, i.e. the causes of accident, radiological examination results, intracranial lesions, operation techniques and outcome are analysed and discussed. The analysis of cases with dural sinus injuries shows a high mortality rate (total mortality rate: 16 patients = 41%; intra-operative mortality rate: 8 patients = 20%).

Adolescent↗
PubMed1992

Drug rechallenge and patients' rights.

Challenging a hypersensitive patient with a potentially harmful drug is most important to the development of medical knowledge. However, it does not always serve the interest of the particular patient. In order to protect the interest of an individual patient, one must abstain from challenge unless it is vital to the health of the particular patient at the time and has no substitute. Patients' rights demand the establishment of limitations and guidelines for the procedure of rechallenge in all its aspects. An approach which will bring physicians closer to a solution, which will protect the patient from uncontrolled decisions and from unnecessary risks, is suggested.

Clinical Protocols↗
PubMed1991

Atherosclerosis in the aorta of hypercholesterolemic rabbits and the influence of daltroban.

These studies have examined aortic atherogenesis in cholesterol-fed rabbits and have correlated the effects of daltroban to the pathomechanism of the vessel wall lesions. After feeding a 0.5% cholesterol-enriched diet for 96 d atherosclerotic alterations were seen, which exhibited a proximo-distal pattern, to which the branching of the aorta contributed considerably. Depending on their localization and size a varying cellular constitution of the plaques was obvious. Large plaques, which were mainly seen in the aortic arch and the proximal descending thoracic aorta, consisted of numerous proliferating cells, masses of fibrillar ground substance, clusters of foam cells, and rarely contained cholesterol crystals and necroses. Emerging plaques mainly found in distal thoracic and abdominal aorta imposed as fatty streaks. Daltroban treatment, used in a clinically relevant doses of 10 mg/kg b. wt. per day, reduced extension and protrusional area of plaques to about 40%, which was evaluated using a newly developed computerized morphormetric method, in association with significant reductions in free cholesterol content within the aorta. The results suggest that daltroban inhibits the progression of atherosclerosis in cholesterol-fed rabbits. This effect may be related to its antagonistic interaction with the thromboxane A2 receptor and also to an inhibition of the cholesterol metabolism.

Animals↗
PubMed1991

[Results of multistep oxygen therapy in the treatment of sudden hearing loss].

Oxygen multistep therapy (von Ardenne) was applied in 28 patients suffering from an idiopathic sudden hearing loss. The oxygen therapy consisted of a multistep short procedure, each of which lasted for 15 minutes. The results of our study were evaluated by means of standardised statistics confirming the effectiveness and even the superiority of the oxygen multistep therapy in comparison with any other treatment, and also in view of the spontaneous remission rate in cases of sudden hearing loss.

Adult↗
PubMed1990

[Comparison of plethysmography findings before and following varicose vein surgery].

In the chronic venous insufficiency on the basis of a primary varicosis the occlusion plethysmography of the veins is a suitable remedy in the positioning trial before and after a combined phlebectomy to determine the capacity of the venous part of the circulation on the lower extremity. In altogether 160 patients the functional capacity of the venous valves with the initial inclination to swelling St as well as the capacity of the venous vascular system on the calf and the ankle were established by the determination of the maximal venous capacity Vmax. It could be proved that particularly in stage III of the chronic venous insufficiency the haemodynamically most effective efficacy can be achieved by an operation of the varices.

Female↗
PubMed1990

Chemotactic response of osteoblastlike cells to transforming growth factor beta.

Transforming growth factor beta (TGF-beta) has multiple effects on bone cell metabolism in vitro but its exact role in bone remodeling still needs to be defined. Here we demonstrate that TGF-beta is chemotactic for osteoblastlike cells from fetal rat calvariae and osteoblastlike ROS 17/2.8 osteosarcoma cells. Maximal chemotaxis occurred at 5-15 pg/ml of TGF-beta and was observed with TGF-beta 1 and TGF-beta 2 at equivalent concentrations. Conditioned medium from osteoblastlike cells containing latent TGF-beta failed to stimulate chemotactic migration. However, chemotactic activity was observed in conditioned medium that had been transiently acidified. Since acidification is known to activate TGF-beta, these results suggest that only active TGF-beta is capable of inducing a chemotactic response. Preincubation of osteoblastlike cells with TGF-beta in concentrations from 10 pg/ml to 1 ng/ml for 48 h abolished a subsequent chemotactic response of these cells to TGF-beta, indicating that TGF-beta-induced chemotaxis is a transient phenomenon. Since TGF-beta may be released from the bone matrix and/or activated during bone resorption, the chemotactic activity of TGF-beta for osteoblastlike cells may be important for the recruitment of osteoblastlike cells to sites of bone remodeling.

Animals↗
PubMed1990

Investigations of the antiatherosclerotic effect of the thromboxane A2 receptor antagonist Daltroban.

In the pathogenesis of atherosclerosis an accumulation of lipids, predominantly in the form of cholesteryl esters within the blood vessel wall is observed. Interaction of the plaques, e.g. with platelets, is suggested to contribute considerably to their growth. Compounds affecting both processes should prevent atherosclerotic progression and therefore are of great interest for potential therapeutic use. We investigated the effect of Daltroban, which has been characterized as a selective thromboxane receptor antagonist with platelet inhibiting activity, in two models: 1) cholesterol metabolism in liver cells and 2) progression of atherosclerosis in aorta of hypercholesterolemic rabbits. Daltroban reduced 14C-acetate incorporation into cholesteryl esters stronger than into cholesterol in rat hepatocyte monolayer cultures. In male white New Zealand rabbits fed 0.5% cholesterol enriched diet for 96 days, coadministration of Daltroban beginning at the 42th day on diet reduced aortic cholesterol content, plaque covered surface of aortic wall, and lumen stenosis by plaques more than 30 percent. The significant inhibition of the progression of atherosclerosis in the hypercholesterolemic rabbits by Daltroban is suggested to be effected by both inhibition of cholesterol metabolism as well as of platelets.

Animals↗
PubMed1990

[Results of the analysis of a regional noise-exposure outpatient unit].

An evaluation of our regional register of noise-exposed persons comprehended within the framework of medical ability and control examinations of workers was given here. On the occasion of medical ability examinations of workers at the beginning of their employment 448 persons were examined. 49 of them had to undergo an additional ENT investigation because working under noise conditions in future. 5.4 p.c. of those were not passed as fit for working in noise environment. On the occasion of medical control examinations of workers during their employment 1893 persons were examined now. 184 of them had to undergo an additional ENT investigation because of their exposition to noise. Following that, it was necessary for 1.4 p.c. of the latter to change their working place, but for 0.6 p.c. of them an announcement to suspicion of occupational hearing damage must be made. In connection with the data above mentioned great attention must be payed to early ear diseases on the one hand, and to hearing damages existing before on the other hand.

Adult↗
PubMed1987

Expression of the gene for the beta subunit of mouse thyrotropin results in multiple mRNAs differing in their 5'-untranslated regions.

The gene encoding the beta subunit of mouse thyrotropin (TSH beta) has been isolated from a mouse genomic library, and its nucleotide sequence has been determined. Blot hybridization analysis of restriction enzyme digests of mouse DNA indicates that there is a single mouse TSH beta gene. The gene is 4.8 kilobases in length and contains five exons, which are 27, 47, 41, 163, and 328 base pairs long. Exons 1, 2, and 3 encode only 5'-untranslated mRNA sequences and are separated by introns that are 150 and 380 base pairs long. The protein-coding mRNA sequences are found in exons 4 and 5 and are interrupted by a 460-base pair intron. The position of this intron, between the codons for amino acids 34 and 35, has been conserved in all the known glycoprotein hormone beta subunit genes. Exons 3 and 4 are separated by a large 3.2-kilobase intron. When primer extension analysis, using an oligonucleotide primer complementary to exon 4 sequences, was employed to locate the transcription start site, four products were obtained. Nucleotide sequencing of these products showed that they were derived from separate TSH beta mRNAs that differed in the lengths of their 5'-untranslated regions. These 5'-untranslated mRNA sequences are derived from different combinations of exons 1, 2, and 3, each spliced to exons 4 and 5. The longest 5'-untranslated sequence, 116 nucleotides long, includes exons 1, 2, and 3 and the first base of exon 4; the shorter 5'-untranslated regions, 75, 69, and 28 nucleotides long, arise by splicing out the second and/or the third exon sequences. In contrast to the mouse TSH beta gene, transcription of the rat TSH beta gene from the analogous start site has been reported to give only a single mRNA, with a 5'-untranslated region of 28 nucleotides. Divergence of the mouse and rat TSH beta gene sequences at RNA splice sites can account for the absence of exon 2, but not exon 3, sequences in rat TSH beta mRNA. Primer extension and RNase protection analyses also showed that the mouse TSH beta gene contains a second transcription start site, located 43 base pairs upstream of the first start site, in a position corresponding to that in the rat TSH beta gene. Each start site in the mouse gene is flanked by characteristic TATAA box and CAAT box sequences.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1986

[111Indium-thrombocyte scintigraphy and percutaneous transluminal dilatation (angioplasty) of supra-aortic vascular stenoses. A new method for deciding therapy and for follow-up control].

Scintigraphy with 111indium-marked autologous platelets has been used during percutaneous transluminal dilatation of supra-aortic arterial stenoses; there were 14 successful dilatations and one attempted dilatation. The material consisted of nine stenoses of the internal carotid artery, two stenoses at the origin of the vertebral artery and four stenoses of the first part of the left subclavian artery. Deposition of thrombi could be demonstrated by scintigraphy, both before and after catheter dilatation. The value of this method for deciding on the type of treatment and for observing the course of percutaneous dilatation of cerebral vessels is discussed.

Angioplasty, Balloon↗