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P A HARTMAN

Publications and source records attributed to P A HARTMAN.

8 recordsLinked to original sources

COMPARISON OF DIRECT PLATING MEDIA FOR THE ISOLATION AND ENUMERATION OF ENTEROCOCCI IN CERTAIN FROZEN FOODS.

A total of 15 agar media were examined for their yield, selectivity, readability, and simplicity of preparation and use. A thallium medium of Barnes was selected as the better of the high yield-fair selectivity type of medium and an azide-citrate medium of Reinbold appeared to be the better of the low yield-high selectivity type of medium. Sodium carbonate (optimal concentration, 0.20%) was found to increase recovery substantially when added to certain media, especially in the presence of 0.05% Tween 80. When these two ingredients were incorporated into a medium modified after Slanetz and Bartley, the resultant medium was superior to other media for the isolation and enumeration of enterococci in certain frozen foods, such as peas and hamburger, by the direct plating method.

Azides↗

Formation of monothetic groups in quantitative bacterial taxonomy.

Lockhart, W. R. (Department of Bacteriology, Iowa State University, Ames) and P. A. Hartman. Formation of monothetic groups in quantitative bacterial taxonomy. J. Bacteriol. 85:68-77. 1963.-Previous applications of quantitative methods to bacterial classification have resulted in polythetic groupings in which no organism necessarily has all the features characteristic of its group, and no single property is necessarily possessed by every member of a group. Such classifications are not altogether analogous to present taxonomies, and (since the possible groups are not mutually exclusive) hence cannot be evaluated completely by computer methods. A technique is presented for formation of hierarchical monothetic groups in which the criterion for addition of each new member is the possession of an array of properties common to all organisms already in the group. These monothetic relationships among individuals are expressed in terms of cumulative difference, which is linearly related to the taxonomic distance (a function of similarity ratio) of polythetic classifications. Monothetic groupings obtained for 50 representative microorganisms were essentially similar to the polythetic groups evaluated by earlier methods. The necessary computation is suitable for analyzing relationships among large numbers of organisms.

Bacteria↗