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Biomedical subjects

P A Leach

Publications and source records attributed to P A Leach.

5 recordsLinked to original sources

Abolition of resistance to Klebsiella pneumoniae by anti-polymorphonuclear leucocyte IgG.

Antipolymorphonuclear leucocyte IgG (APS IgG) was prepared from crude horse antipolymorphonuclear leucocyte antiserum (APS). The APS IgG was less toxic than the crude APS and had a powerful and specific effect on rabbit polymorphs in vivo and in vitro. In rabbits infected with Klebsiella pneumoniae it suppressed the normal polymorph response and greatly reduced resistance to infection. Anti-Klebsiella pneumoniae antibody greatly enhanced protection in normal rabbits, but had much less effect on APS/IgG-treated rabbits. It was concluded that the principal cellular defence against K. pneumoniae was the polymorph and that macrophages made only a relatively small contribution to resistance.

Animals↗

Anaerobic CO 2 cabinet for the cultivation of strict anerobes.

The design and construction of an anaerobic CO(2) cabinet are described. Air is displaced by a stream of oxygen-free CO(2), and anerobic conditions are produced in 3 hr. The equipment is simple and cheap to operate and has been found to be satisfactory for the isolation of strict anaerobes from the mouse intestine.

Anaerobiosis↗