Quantification of imipramine and desipramine in plasma by high-performance liquid chromatography and fluorescence detection.
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Chromatography, High Pressure Liquid↗
Biomedical subjects
P A Reece
Publications and source records attributed to P A Reece.
Interference in assays for hydralazine in humans by a major plasma metabolite, hydralazine pyruvic acid hydrazone.
The present study showed that published spectrophotometric and GLC methods for hydralazine in plasma do not distinguish between the drug and a major plasma metabolite, hydralazine pyruvic acid hydrazone. These methods involve the acid treatment of the sample, which hydrolyzes that hydrazone back to hydralazine. A specific GLC assay for the hydrazone was developed and involves its selective extraction from plasma and transformation to 3-trifluoromethyl-s-triazolo[3,4-a]phthalazine. This derivative could be sensitively measured by GLC using an electron-capture detector. With this procedure, it was shown that most "apparent hydralazine" in plasma is the hydrazone, which forms rapidly from hydralazine and endogenous pyruvic acid. Previous work indicated that the hydrazone was inactive when administered intravenously to rabbits.
Chromatography, Gas↗
Purine studies. Part XX. Methylation and reduction of 2,8-dioxo-, 2,8-diamino-, and 2-amino-8-oxo-purines, and the stereochemistry of their 1,4,5,6-tetrahydro-derivatives.
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Hydroxylation↗