Outcomes data collection technology: observations and lessons from year one.
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Contract Services↗
Biomedical subjects
P Alles
Publications and source records attributed to P Alles.
CESF Medical Outcomes Research Project: implementing outcomes assessment in a clinical setting.
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Data Collection↗
A search for immunoreactive substance P and other neural peptides in the limb regenerate of the newt Notophthalmus viridescens.
Immunochemical studies demonstrate that the undecapeptide substance P (SP) may be detected by radioimmunoassay in newt limb regenerates and that SP is localized in the blastemal epidermis by immunofluorescence and peroxidase-antiperoxidase staining. Immunoreactive SP is predominantly distributed at the periphery of epidermal cells, suggesting the presence of SP binding sites on the cell surface; the basal germinative layer of the epidermis and blastemal mesenchyme cells remain unreactive. The pattern of SP immunoreactivity in the blastema was compared with that of four other tachykinin-family peptides (eledoisin, kassinin, substance K, and neuromedin K) and with three non-tachykinin neural peptides (bombesin, neurotensin, and metenkephalin). With the exception of neurotensin, which showed weak staining in the basal layer but an absence in the peripheral layers of the epidermis, none of the peptides examined exhibited immunoreactivity in the blastema epidermis comparable to that of SP.
Animals↗
A rapid procedure for the purification of IgA1 and IgA2 subclasses from normal human serum using protein G and jackfruit lectin (jacalin) affinity chromatography.
Immunoglobulin A (IgA) from pooled normal human sera was purified using antibody and protein G affinity chromatography and gel filtration high-pressure liquid chromatography (HPLC). This high purity product was separated into IgA1 and IgA2 subclasses utilizing the agarose-bound lectin 'jacalin'. Evaluation of product homogeneity by immunological testing confirmed greater than 95% purity. The total IgA1 and IgA2 recovered from sera was approximately 26% of the initial antibody present.
Chromatography, Affinity↗