Biomedical subjects
P Anderton
Publications and source records attributed to P Anderton.
Measles-virus-persistent infection in BGM cells. Modification of the incorporation of [3H]arachidonic acid and [14C]stearic acid into lipids.
In BGM cells chronically infected with measles virus, although the composition of the phospholipids is unaltered, the fatty acid composition is modified. Uninfected, lytic and persistently infected cells were labelled with [3H]arachidonic acid and [14C]stearic acid and their metabolic fate analysed. No difference in the total incorporation was observed in the different systems. However, the [14C]stearic acid and [3H]arachidonic acid were incorporated up to 2-fold and 13-fold respectively greater into the neutral lipid of persistently infected compared with that of uninfected cells. Both radioactive fatty acids were specifically accumulated in the triacylglycerol and non-esterified fatty acids fractions. Lytically infected cells were similar to uninfected cells. Although there was no significant difference in the incorporation of radioactivity into the total phospholipid in either system, there was a large decrease in [3H]arachidonic acid incorporated into phosphatidylethanolamine and to a lesser extent phosphatidylcholine and phosphatidylinositol in persistently infected cells. [14C]Stearic acid incorporation was also reduced in phosphatidylcholine and phosphatidylethanolamine fractions of persistently infected cells.
Accumulation of radiolabelled fatty acids in the neutral lipid fraction of measles virus persistently infected BGM cells.
The effect of measles virus infection (acute and persistent) on fatty acid metabolism has been studied in BGM (African green monkey kidney) cells. In persistently infected cells, there was an increase in the incorporation of radiolabelled fatty acids into the neutral lipid fraction. Compared to uninfected cells, the increase was up to 2-fold for palmitic, stearic and oleic acids and 8-14 fold for arachidonic acid. The lipid metabolism in measles virus acutely infected cells was unmodified. The radiolabelled fatty acids incorporated into the neutral lipids in persistently infected cells were principally associated with the triglyceride fraction. The implications of a virus- induced modification of lipid metabolism are discussed.
Phospholipids in a measles virus persistent infection: modification of fatty acid metabolism and fatty acid composition of released virus.
The phospholipid metabolism of a measles virus persistently infected cell line, BGM/Hallé, was compared with that of uninfected BGM cells. Synthesis of phospholipid from the isotopic precursor [32P]phosphate was unaffected, but a significant increase in the synthesis of phospholipid from [3H-9,10(n)]palmitic acid was observed in persistently infected cells, reflecting an increase in the palmitic acid content of phospholipid previously described for these cells. The phospholipid composition of measles virus released from persistently infected cells was compared to that of virus from lytically infected BGM cells by radiolabelling to isotopic equilibrium and measuring the incorporation of labelled phosphatides into the virus particle. The incorporation and distribution of [32P]phosphate-labelled phosphatides in virus was similar in lytic and persistent infections. In contrast, the distribution of [3H]palmitic acid among the constituent phosphatides of virus released from the persistent infection was different, partly reflecting the altered saturated fatty acid composition of the phosphatides of the host cell.
Modification of the fatty acid composition of phospholipid in measles virus-persistently infected cells.
Explore the source record for details and available documents.