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Biomedical subjects

P Antonio

Publications and source records attributed to P Antonio.

14 recordsLinked to original sources
PubMed1989

Salivary corticosteroids in the study of adrenal function.

Salivary corticosteroids (SCC) and plasma corticosteroids (PCC) were studied under basal conditions, after dexamethasone (DXM) and in the ACTH stimulation test in a reference group (RG) of 33 adults, in three groups with non-adrenal pathology and in a group of 4 patients with hypercortisolaemia. SCC and PCC were measured using a non-extraction RIA method using [3H]cortisol. The results for SCC in the RG and in the groups with non-adrenal pathology were similar to those obtained for PCC in terms of percentage of decrease in the circadian rhythm or DXM suppression. However, the responsiveness to ACTH in saliva was twice that obtained in plasma. In patients with hypercortisolism, SCC were in closer agreement with the adrenal hyperfunction than PCC. From the previous results the following conclusions may be drawn: (a) SCC differentiate adrenal gland normal function from hyperfunction as clearly or even better than PCC does; (b) SCC were in a closer agreement with the symptomatology of adrenal hyperfunction than were PCC; and (c) the responses to ACTH obtained with SCC were clearly higher than those obtained with PCC.

Adenoma↗
PubMed1989

Plasma sex steroids and SHBG in patients with breast cancer and their relation to tumor oestrogen-dependency.

In order to evaluate the possible role of sex steroids in breast cancer and their relation to the presence of oestradiol receptors (ER), ER were quantified in 112 breast adenocarcinomas and 14 benign tumours. The sex hormones of the patients were determined in plasma: oestradiol (E2), progesterone, testosterone, androstenedione, as well as SHBG and gonadotrophins. The ER were evaluated in cytosolic and nuclear fractions. Steroids and gonadotrophins were determined by RIA. SHBG was determined by following a procedure of saturation with tritiated dihydrostestosterone. The mean levels of E2 in postmenopausal women with breast cancer was found to be significantly higher than of the control group. However, no significant variations for the rest of the steroids were seen among the patients with breast cancer and the control group or benign tumour. Nor was any relation found between the presence of ER and the hormonal steroids studied or SHBG. It is suggested that the possible influence of the sex steroids in the etiopathogenesis of breast cancer might be due to the greater production accompanied by a parallel metabolic clearance that would maintain their plasma levels.

Adenocarcinoma↗
PubMed1988

Production of specific retinal S antigen antibodies in patients with retinal detachment.

One hundred patients with retinal detachment (61 primary operations and 39 reoperations) were studied for titers of autoantibodies to human retinal S antigen using the enzyme-linked immunosorbent assay technique (ELISA). There was no statistically significant difference when comparing the group of patients with primary retinal detachment with the control group. However, a statistically significant increase in anti-S titers was recorded when a comparison was made between the group of reoperated patients and the control group (chi 2, P less than 0.001) and between the group of reoperated subjects and the group of patients operated on for the first time (chi 2, P less than 0.005).

Antibody Specificity↗
PubMed1985

Basal prolactin and the behaviour of the gonadotrophins, testosterone, androstenedione, estradiol, and the sex-hormone-binding globulin during stimulation with clomiphene in subjects with spermatogenic disorders.

To clarify the significance of clomiphene test in spermatogenic disorders, it was performed on three groups of subjects: 10 with normozoospermia, 29 with oligozoospermia, and 11 with azoospermia. Two basal blood samples were drawn five days apart; prolactin, FSH, LH, estradiol, testosterone, androstenedione and sex-hormone-binding globulin were determined. 100 mg of clomiphene per day were administered for eleven consecutive days; another sample was drawn on the eleventh day and all the basal parameters, except prolactin, were determined. It may be concluded from the results: a) All the parameters studied increased significantly after clomiphene; and b) Testosterone levels obtained after clomiphene, as well as the increases in this hormone during the test and the ratio delta T/delta LH, were significantly lower in the oligozoospermic group. This finding suggests a decrease in the testicular androgenic function of this group.

Adult↗
PubMed1983

DNA/protein ratio as an index of oestrogen receptor content in human breast cancer.

The nuclear DNA/cytoplasmic protein ratio was related to the oestradiol receptor content in both cytoplasmic (ERc) and nuclear (ERn) fractions in 165 cases of breast cancer. The patients under study were divided into groups according to whether their biopsies contained functional (ERc/ERn = +/+), abnormal (+/0) or non-detectable receptor (0/0). The mean microgram DNA/mg protein ratio was significantly decreased in both the abnormal and receptor-negative groups, with means of 30.4 and 30.2 respectively, when compared to the mean of 52.8 for the receptor-positive group. When the DNA/protein ratio for each patient was related to age, a discriminant line which clearly differentiated the ER +/+ group from the other two groups was obtained. It may be concluded that an increased DNA/protein ratio is related to the presence of ERn. Furthermore, since the DNA content per cell is higher in ER-negative tumours, an increase in cellularity must exist in ER-positive tumours.

Adult↗
PubMed1983

Role of the ovary in the regulation of sex hormone binding globulin and its contribution to peripheral levels of androstenedione.

In order to assess the role of the ovary in the regulation of the levels of the sex-hormone-binding-globulin (SHBG) and its contribution to peripheral levels of androstenedione, three subject groups were studied: 10 normally menstruating women, 8 postmenopausic women, and 8 postmenopausic ovariectomized women. A basal venous blood sample was drawn between 8:00 to 9:00 h from all subjects in the latter two groups. The blood samples were drawn from the normally menstruating women on days 7, 14, 21 and 28 of their cycles. The following determinations were made in each sample: oestradiol, progesterone, testosterone, androstenedione SHBG, FSH and LH. It was concluded that: 1) SHBG does not vary during the menstrual cycle, nor there are significant differences between postmenopausic and castrated women, and 2) androstenedione levels in castrated women are significantly different from both postmenopausic and normally menstruating women.

Adult↗
PubMed1980

Disposition of a new steroidal anti-inflammatory agent, deflazacort, in rat, dog and man.

The physiological disposition of a new steroid anti-inflammatory agent, deflazacort, was examined in rat, dog and man following 5 mg/kg doses to the animals, and 50 mg to humans. The administered radiocarbon [2'-14C]-deflazacort), is rapidly and extensively absorbed into the general circulation in rat and man, whereas the bioavailability in the dog is low. The terminal plasma half-life for radioactivity elimination was, on the average, 11, 15 and 28 hr in rats, dogs and man, respectively. Urinary excretion was the predominant route of 14C elimination in the rat (-54% of the dose) and in man (-68% of the dose), whereas in the dog the majority of the dose was eliminated via the feces (82%). Tissue distribution studies in the rat did not show target organs, with the exception of the blood cells. Studies of binding to plasma proteins of the 21-desacetyl deflazacort, demonstrate in all the species a rather low level of binding of non-saturable type.

Adult↗