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P Bílek

Publications and source records attributed to P Bílek.

9 recordsLinked to original sources
PubMed1998

[Development of dipeptidyl peptidase IV (DPP IV) activity in postnatal differentiation of the submandibular gland in mice].

The localization of a serine exopeptidase--DPP IV--in the male and female submandibular gland of the mouse during gland postnatal development was studied histochemically in the light microscopic level. The present results suggest that localization of DPP IV is closely related to the postnatal differentiation and maturation of acini and male granular convoluted tubules. Remarkable sex differences of DPP IV activity were detected in the submandibular gland of pubescent and adult animals. The possible physiological role of DPP IV in major salivary glands is also discussed in the paper.

Aging↗
PubMed1998

[Histochemical activity of dipeptidyl peptidase IV (DPP IV) in intracerebral transplantation of the submandibular gland in mice].

Histochemical activity of DPP IV in the intracerebral homotransplants of submandibular gland of the newborn mouse was investigated in the course of a 5-month period after transplantation. Eight weeks after grafting, the enzyme pattern in the parenchyma of transplants was already comparable with the enzyme pattern of a fully mature mouse submandibular gland in situ. At this time, DPP IV was active in the apicolateral cell membranes of numerous acini of the gland transplants. Enzyme activity in apical cell cytoplasm of granular convoluted tubules was found only in transplants located in the brain of male recipients.

Animals↗
PubMed1998

Activity of alkaline phosphatase in the major salivary glands of mice at various ages of postnatal life, and during pregnancy and lactation.

Activity of alkaline phosphatase in the major salivary glands of male and female mice at various ages of postnatal life, and in females during lactation was studied histochemically. Enzyme activity was not detected on the day of birth, but was found in the terminal tubules of all major salivary glands during the first postnatal week. Alkaline phosphatase activity was increasing gradually with age and a definitive enzymatic pattern was observed by the age of 6 weeks. No difference in enzyme activity was found among the major salivary glands of young adult and old animals. The parenchyma of fully differentiated submandibular glands showed clear sexually dimorphic patterns of alkaline phosphatase activity. During pregnancy, a significant increase of alkaline phosphatase activity was detected in submandibular gland. From gestation day 15 to the end of pregnancy, enzymic pattern of granular convoluted tubules of pregnant females was the same as in the adult males. Histochemical masculinization of the submandibular gland during pregnancy suggests that besides androgens also progesterone exerts masculinization of the murine submandibular salivary gland.

Aging↗
PubMed1995

Histochemical localization of succinate dehydrogenase in the mouse submandibular gland during postnatal development.

The activity of succinate dehydrogenase (SDH) was examined histochemically in the parenchyma of murine submandibular gland (SMG) during postnatal development on light-microscopical level. In this study, SDH appears as a good marker of differentiating and mature striated ducts of both sexes and convoluted granular tubules of males. Enzyme activity in glandular parenchyma increased from the 1st day to the 7th week of postnatal life. Definitive enzymatic pattern was seen in the gland aged 7 weeks. Histochemical differences between male and female SMG of mouse were noted.

Aging↗
PubMed1995

Histochemical localization of succinate dehydrogenase in intracerebral mouse submandibular gland transplants.

Histochemical localization of succinate dehydrogenase in developing intracerebral auto- and homotransplants of the mouse submandibular gland was investigated in the course of a 5-month period after transplantation. Eight weeks after grafting, the enzyme pattern in the parenchyma of homotransplants was comparable with the histochemical picture of a fully mature submandibular gland in situ. At this time, numerous acini showed a weak activity, very numerous striated ducts a strong activity, and less frequent developing convoluted granular tubules slightly weaker activity, than in the striated ducts. Beginning histochemical differentiation of convoluted granular tubules was noted only in homotransplants, located in the brain of male recipients. On the other hand, only a weak activity in the cytoplasm of non-differentiated duct--like structures of some autotransplants was seen. Homotransplants of non-differentiated submandibular gland of newborn donors were found to be a more suitable transplantation object capable of postnatal development of gland parenchyma than autografts of fully differentiated gland of adult animals that did not enter the cytodifferentiation stage.

Animals↗
PubMed1992

Cuticular spinning structures reveal evolutionary relationships in araneomorph spiders.

The present paper reviews the results from the study of spigots, i.e. cuticular spinning structures obtained by the scanning of 52 genera belonging to 28 families of araneomorph spiders. The finding of eight morphologically different forms of these structures being attached to ducts of the pyriform type of silk glands is of fundamental importance for the final conclusions. The comparative observations enable me to create a hypothesis dealing with the existence of several evolutionary lines of spiders, each of which is represented with their own form of spigots. Nevertheless the character of these spigots undoubtedly reflects phylogenetic relationships between the spider taxa. The detection of a special mechanoreceptive field on top of the anterolateral spinnerets increases the knowledge of the functional morphology of the spider's spinning apparatus.

Animals↗
PubMed1989

[Histochemical detection of enzymes in the spinning glands of spiders].

The spider's spinning apparatus equipped with numerous glands of several types appears to be a suitable model for studying tissues which produce large amounts of fibrillar proteins. Heterogeneous glandular system is of special benefit for study of the function of different enzymes taking part both in the synthesis and the degradation of mentioned proteins. It is evident that the activity of the enzyme is dependent not only on the physiologic condition or a certain phase of spider's life cycle, but it is also given by the type of gland, i. e., defined by the structure and properties of protein to be emitted. Histochemical study of enzymes brings some new and more precise views on the spinning apparatus function. Also a participation of individual gland types in some definite spider's life period is so indicated as well as it is convenient for better understanding biologic function of structures performed by spider.

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