AIDS and taurolin.
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Biomedical subjects
Publications and source records attributed to P Bayardelle.
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The route of infection in acute suppurative thyroiditis is unknown in most cases; when demonstrated, pyriform sinus fistula appears to be the most frequent one. We report the clinical and laboratory findings of a child in whom culture of the thyroid pus yielded two bacteria which are part of the normal oropharyngeal flora: capnocytophaga ochracea and group F Beta-hemolytic streptococcus. The preliminary results of the culture, which showed a mixed flora, prompted us to search and to find a pyriform sinus fistula. Apart from the onset in infancy, the left lobe involvement and the frequent recurrence, the recovery from the thyroid pus of bacteria from normal oropharyngeal flora should be included in the characteristic features of thyroiditis resulting from an infection through the pyriform sinus fistula.
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A total of 1,002 urine specimens were evaluated by laser nephelometry. This technique was compared with both colony counts, done with a calibrated loop, and serial dilutions. For urine specimens containing between 10(4) and 10(5) bacteria per ml, laser nephelometry detected 75.4% of those detected by colony count and 65.6% of those detected by serial dilution. For specimens where the concentration of bacteria was greater than 10(5) per ml, laser nephelometry detected 95.8 and 92.4% of those detected by colony count and serial dilution, respectively. The mean detection time for bacteriuria varied from 1.57 h for more than 10(5) bacteria per ml to 4.47 h for more than 10(4) bacteria per ml. To determine the number of bacteria according to the voltage growth curve, the passage time at 3 V was used as an index. The mean passage time at 3 V decreased from 5.18 h for fewer than 10(4) bacteria per ml to 1.42 h for more than 10(6) bacteria per ml. The mean passage time at 3 V differed significantly for different concentrations of bacteria. Thus, this index allowed us to predict the number of bacteria in the urine specimens. Laser nephelometry has been used for many years for the immunological determination of proteins; it can now also be considered a tool for rapid screening in bacteriology.
Detection of significant bacteriuria with a laser nephelometer was evaluated in this study and compared with the results obtained by the quantitative loop method. We screened 1002 urine specimens and 220 (21.95%) were found to be positive at greater than or equal to 10(5) colony-forming units (CFU)/mL of urine by the standard method. Of the 220 positive specimens, 210 (95.4%) were detected in 6 h or less and 177 (80.4%) were detected within 3 h. The false-positive rate was 2.3% at 3 h and 19.7% at 6 h. These findings suggest that a 6-h procedure is necessary to detect 95% or more of significant bacteriuria. Laser nephelometer is versatile and can be used for rapid screening of bacteriuria.
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The early detection of Gram-negative rod bacteremia favors the prompt institution of appropriate antimicrobial therapy. During a 6-week period, blood cultures were subcultured in broth during the first 18 h of incubation. The turbidity of these subcultures was studied by laser nephelometry. Fifty-five percent of the blood cultures positive for Enterobacteriaceae were reported on the same day the sample was taken from the patient; this constitutes a marked acceleration in comparison with the results obtained by a conventional method. The identification and results of the antimicrobial susceptibility tests were available 2 days after the specimen was taken. Early subculture in broth combined with laser nephelometry studies of broth turbidity is readily applied to the study of bacteremia due to Enterobacteriaceae and may favorably influence the outcome of such disease by providing specific results more rapidly.
A 26-year-old woman had an enlarging dense macular lesion in the right eye. The funduscopic and angiographic findings were distinctly different from those in macular histoplasmosis. She was found to have a coccidioidomycotic granuloma in the left lung and was treated with amphotericin B. Within a month the macular lesion was cicatricial. This appears to be the first reported case of presumed ocular coccidioidomycosis in Canada.
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