Biomedical subjects
P Benedict
Publications and source records attributed to P Benedict.
Effects of antibiotic treatment in the subset of common-cold patients who have bacteria in nasopharyngeal secretions.
BACKGROUND: Upper-respiratory-tract infection is one of the main causes of overuse of antibiotics. We have found previously that bacteria such as Haemophilus influenzae, Moraxella catarrhalis, and Streptococcus pneumoniae can be isolated from the nasopharyngeal secretions of a substantial proportion of adults with upper-respiratory-tract infections. We have assessed the efficacy of co-amoxiclav in patients with common colds but no clinical signs of sinusitis or other indications for antibiotics. METHODS: Between January, 1992 and March, 1994, 314 patients who presented to our outpatient clinic with common colds were enrolled in the double-blind, placebo-controlled study. They were randomly assigned 5 days' treatment with co-amoxiclav (375 mg three times daily) or identical placebo. Clinical examinations were done at enrolment and on day 5-7 to assess outcome (cured, persistent symptoms, worse symptoms). Seven patients were excluded after randomisation, seven did not have nasopharyngeal aspiration, and 12 did not return for followup assessment. FINDINGS: Of 300 patients with nasopharyngeal aspirates, 72 had negative bacterial cultures, 167 had cultures positive only for bacteria unrelated to respiratory infections, and 61 had cultures positive for H influenzae, M catarrhalis, or S pneumoniae. At 5-day follow-up of these culture-positive patients, the distribution of outcome was significantly better among co-amoxiclav-treated (n=30) than placebo-treated (n=28) patients (cured 27 vs 4%; persistent symptoms 70 vs 60%; worse symptoms 3 vs 36%; p=0.001). Patients on co-amoxiclav also scored their symptoms significantly lower than patients on placebo (p=0.008). Among culture-negative patients (n=230), the outcome distribution did not differ between the treatment groups (p=0.392). INTERPRETATION: The majority of patients with upper-respiratory-tract infection do not benefit from antibiotics and side-effects are frequent. However, for the subgroup whose nasopharyngeal secretions contain H influenzae, M catarrhalis, or S pneumoniae, antibiotics are clinically beneficial.
Aflatoxin B1-adduct formation in rat and human small bowel enterocytes.
BACKGROUND/AIMS: Hepatic CYP3A enzymes have been implicated in the bioactivation of aflatoxin B1 (AFB1) to DNA binding metabolites. CYP3A enzymes are also abundant in the small bowel, and we therefore examined the ability of this tissue to form intracellular AFB1 adducts. METHODS: Immunohistochemistry using a antibody to the stable AFB1-DNA adduct was performed on small bowel sections obtained from rats orally gavaged with AFB1 and on human small bowel biopsy specimens maintained in explant culture. 3H-AFB1 was instilled into a loop of small bowel of untreated rats and rats pretreated with the CYP3A inducer dexamethasone during vivisection. DNA was isolated from the loop 2 hours later and assayed for specific activity. RESULTS: In both rats and humans, AFB1-adducts were detected exclusively in mature enterocytes in a pattern similar to the distribution of CYP3A enzymes. Induction of enterocyte CYP3A in rats resulted in an increase in enterocyte immunoreactive AFB1 adducts and in a 1.8-fold increase in 3H-AFB1-nucleic acid adducts (P = 0.01). CONCLUSIONS: Intracellular AFB1 adducts are formed in the small intestine, and this reflects, at least in part, the catalytic activity of CYP3A enzymes. Because these AFB1 adducts should ultimately pass in stool, enterocyte CYP3A may represent a regulatable barrier to dietary aflatoxins.
Contributions of persons with serious mental illness to their families.
OBJECTIVE: This study investigated the contributions that persons with serious mental illness make to their families according to both clients and family respondents. METHODS: The sample consisted of 725 clients with serious mental illness and 725 family respondents living in rural counties or counties with small urban areas in Wisconsin. Clients were in contact with family respondents at least three times a year; 23.7 percent of them lived with the respondent. Clients returned a questionnaire, and family respondents completed a telephone interview; both rated the amount of help the client gave in eight areas such as household chores, shopping, and companionship. RESULTS: Overall, the clients, especially those who lived with their families, provided substantial help. For the total sample, clients were most likely to contribute by providing companionship; family respondents reported that 59 percent of clients gave such help. For clients who lived with respondents, between 50 and 80 percent helped by doing household chores, shopping, listening to problems, providing companionship, and providing news about family and friends, according to family respondents. CONCLUSIONS: Many persons with severe mental illness play positive roles in their families. Recognition of clients' contributions by providers, researchers, and clients and families themselves could help reduce stigma and expand community opportunities for persons with severe mental illness.
Postop teaching for the colostomy patient.
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Brief or new: egress during fire--wheelchair exiting in an emergency.
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