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P Bhat

Publications and source records attributed to P Bhat.

At least 37 records · Page 2Linked to original sources
PubMed1985

Coagglutination and counter immunoelectrophoresis in the rapid diagnosis of typhoid fever.

The efficacy of two methods--coagglutination (COAG) and counter immunoelectrophoresis (CIE)--in the rapid diagnosis of typhoid fever was studied in parallel with blood and clot cultures on 114 clinically suspected cases. Retrospective analysis showed that only 58 eventually were discharged and had typhoid fever. Antigen detection on their sera was done by both methods, concomitant with antigen detection on culture supernates by CIE. Sera from 50 controls were subjected to both tests. Agglutinating anti-serum being unsatisfactory in the CIE system, anti-serum to the LPS fraction of Salmonella typhi "O" 901 was used in both tests after absorption with Escherichia coli and Salmonella paratyphi A. Analysis of data with reference to retrospectively confirmed typhoid cases show that S. typhi was isolated in 58.6% and 58.3% of blood and clot cultures; antigen detection by CIE in their supernates was 81.1% and 79.2%, respectively. This correlated closely with serum COAG (81.0%) in contrast to serum CIE (5.7%). Thus, COAG was superior to CIE for serology. However, CIE done on culture supernates precludes such tedious procedures as absorption of staphylococcal agglutinins and the confirmatory blocking test.

Agglutination Tests↗
PubMed1984

A common-source epidemic of mixed bacterial diarrhea with secondary transmission.

An epidemic of acute diarrhea in a village in southern India in 1972 was associated with a high rate of isolation of enteric pathogenic bacteria from the patients and lasted for three months. There was no significant association between the prevalence of enteroviruses or parasites and cases of diarrhea. The epidemic started as a common-source outbreak due to the contamination of well water, and there were many secondary cases probably due to a person-to-person spread. The illness did not produce chronic diarrhea or malabsorption.

Adolescent↗
PubMed1983

Seroepidemiology of malaria in Karnataka State: longitudinal study of a population from an area with high incidence at Kolar, South India.

Serologic methods have considerable value in epidemiologic surveillance of malaria. Antigens prepared from P falciparum cultured in vitro have been found to be sensitive and to a certain extent specific in the IFA test to detect malarial antibodies in the sera from a population continuously exposed to P falciparum and Plasmodium vivax infections [1]. However, it is necessary to assess the serologic data against the results of conventional examination of peripheral blood smears in monitoring the effect of antimalaria measures on malaria transmission. With this objective, we studied a randomly selected population from GPHU, which is located in an area in the Kolar District of the state of Karnataka where malaria is known to be endemic. During the transmission period of 1980 (from July to November), the annual parasitic incidence was 202 per 1,000, with 78% due to P vivax and 22% to P falciparum. The antimalaria measures were subsequently intensified by the GPHU staff, which lead to a decrease in the incidence of malaria in 1981 (60 per 1,000, with 91% due to P vivax and 9% to P falciparum; data obtained from GPHU records). Serum samples were collected after each transmission period and tested by IFA using a local isolate of P falciparum as antigen. There was a marked decrease in the number of seropositive samples in the second batch among children less than 10 years of age and a threefold decrease in malarial antibody titers up to the age of 15 years.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1982

Evaluation of a crude soluble antigen from in-vitro culture of Plasmodium falciparum for ELISA.

The present study was to evaluate the soluble antigen prepared from the in-vitro cultured P. falciparum (FCR3) as an alternative source of antigen to those obtained from in-vivo models for enzyme linked immunosorbent assay (ELISA) in serology of malaria. Results obtained on known positive and negative reference sera revealed good correlation between the ELISA and the indirect fluorescent antibody (IFA) technique (rs = 0.797; p less than 0.001). However such close correlation was not observed on six local sera from patients whose peripheral blood smear showed ring stage of P. falciparum (rs = 0.43; p greater than 0.05), though all the six sera were positive by the IFA and ELISA tests. Test was repeated to establish its reproducibility. The results indicate that antigen prepared from in vitro culture and stored in liquid nitrogen was found sensitive in ELISA for serology of malaria.

Antibodies↗
PubMed1981

Evaluation of in-vitro-cultured Plasmodium falciparum as antigen for malaria serology.

A handicap in developing seroepidemiology for malaria in India is the lack of homologous antigens. Aotus monkeys, in which human plasmodia can be propagated, are not easily available in India. Other alternatives include the use of infected human blood or the in-vitro culture of Plasmodium falciparum, using the candle-jar technique of Trager & Jensen (1976). With certain modifications, the in-vitro culture technique has been established at the St John's Medical College (SJMC) to obtain a regular and high yield of P. falciparum to use as a source of antigen in serological tests. Antigens prepared from in-vitro-cultivated P. falciparum have been tested against positive and negative reference sera by the indirect haemagglutination (IHA) and immunofluorescent antibody (IFA) tests. These reference sera were also tested with the IHA test by using P. falciparum-sensitized human "O' erythrocytes received from the Center for Disease Control (CDC), Atlanta. IHA test results with both types of antigens were comparable (Spearman rank correlation coefficient, rS=0.8440, P less than 0.01). The antigen from in-vitro culture was also found suitable for use in the IFA test. The IFA titres obtained were comparable to those found at the CDC (Spearman rank correlation coefficient, rS=0.7940, P less than 0.01). These results indicate that P. falciparum parasites prepared from in-vitro continuous culture can be used as a source of antigen for use in the IHA and IFA tests for malaria.

Antigens↗
PubMed1980

Bacterial flora of the jejunum: a comparison of luminal aspirate and mucosal biopsy.

The aerobic and anaerobic bacterial flora grown from 40 human jejunal aspirates were compared with the flora grown from an intestinal mucosal biopsy obtained simultaneously from the same level. In four paired samples the flora was identical; in nine the flora differed by only one organism. In six pairs the flora differed by two organisms, and in 11 pairs by three or more organisms. In nine pairs the jejunal aspirate grew only one organism or was sterile, but the biopsy showed considerable numbers of organisms. In one pair the jejunal aspirate grew organisms, but there was no growth from the biopsy. It is apparent that for adequate bacteriological study of the intestine, the flora at both sites should be investigated.

Actinomycetaceae↗