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Biomedical subjects

P Borzini

Publications and source records attributed to P Borzini.

At least 19 recordsLinked to original sources
PubMed1992

Granulocyte serology findings in juvenile symptomatic idiopathic autoimmune neutropenia using a multiassay procedure. Report on 21 cases.

The serological findings on 21 children with idiopathic autoimmune neutropenia are reported. A multiassay procedure was adopted including agglutination, immunofluorescence and cytotoxicity tests. Beside a cause-effect correlation between granulocyte antibodies and clinical course, a serologic polymorphism was found. As the sensitivity of each assay seemed to be related to the characteristics of the involved antigens and antibodies, as well as to the intrinsic sensitivity of the tests, the performance of a multiassay procedure appears to be advisable for the diagnosis and the follow-up of autoimmune neutropenia.

Agglutination Tests↗
PubMed1979

A long-term follow-up study in essential cryoglobulinemia.

In a case series of 56 patients with essential cryoglobulinemia, 35 were followed-up for 4-13 years (mean 7 years). A membranous proliferative glomerulonephritis, which in about half the cases showed a progression to renal insufficiency, was the commonest complication, observed in more than one third of the patients. In 2 patients hepatic cirrhosis became manifest after a completely asymptomatic period and in 2 others a lymphoproliferative disease appeared 2 and 8 years after the onset of purpura. In 51% of patients the intial clinical pattern did not change. In searching for a correlation between the development of nephropathy and cryoglobulin characteristics, none was demonstrated studying the cryoglobulin level, the presence of autoantibody and the complement components.

Adolescent↗
PubMed1978

[Behavior of some parameters of humoral immunity in renal transplants after more than 3 years].

This paper evaluates the humoral immune response of 15 cadaver kidney transplant recipients more than three years after transplantation. The study was performed by determining immunoglobulin levels, complement fractions and circulating antigen-antibody complexes. The data obtained suggest that humoral immunity is impaired in patients who have undergone kidney transplant, even after more than three years later. The impairment of immunocompetence may be ascribed both to the immunosuppressive therapy (corticosteroids and azathioprine) and to the transplanted organ itself which represents a chronic antigenic stimulus.

Adolescent↗
PubMed1978

Occurrence of circulating immune complexes in beta-thalassaemia major.

The presence of circulating soluble immune complexes and the level of complement were investigated in sera from 21 patients with beta-thalassaemia major, including both splenectomised and nonsplenectomised patients. A high level of immune complexes was found in half of these cases. Reduced complement levels were seen less frequently. There was no correlation between the presence of circulating immune complexes, decreased complement levels, and thpresence or absence of the spleen. The level of immune complexes increased with the age ofthe individual, i.e. with the duration of the disease.

Age Factors↗
PubMed1978

Acquired C1-inhibitor deficiency in essential cryoglobulinemia and macrocryoglobulinemia.

In 5 patients with mixed cryoglobulinemia, 3 with Waldenström's disease and 2 with essential cryoglobulinemia, a C1-inhibitor (C1-INH) deficiency was discovered. The complement profile was characteristic of the acquired type: the total hemolytic activity and the early components were reduced, C3 was diminished in 1 patient only, C5 and C9 were normal or elevated. 1 atient with Waldenström's disease and 1 with essential cryoglobulinemia experienced episodes of angioedema. Circulating immune complexes were found in all patients' sera by the 125I-radiolabelled C1q binding activity (C1q BA) test. The values of the C1q binding activity were correlated with the depletion of the early complement components and that of C1-inh. 4 patients showed circulating 7S IgM. Our data support the hypothesis of a complement activation by the cryoprecipitating immune complexes; the C1-INH depletion is secondary to its consumption following C1 activation.

Complement C1 Inactivator Proteins↗
PubMed1977

IgG and C3 receptors on polymorphonuclear leukocytes in phagocytosis. Role of C3-receptors in the attachment and ingestion phases.

The role of IgG and complement (C3) receptors in the adhesion and ingestion phases of immune complexes by normal human polymorphonuclear leukocytes (PMN) were examined. The immune complexes were sheep red cells (E) sensitized with IgG (EA) or IgM antibodies plus complement (EAC'). Both types of receptor sites are involved in the adhesion phase. Moreover IgG receptors are primarily involved in the ingestion phase. Nevertheless even C3-receptors may be sufficient for complete phagocytosis. Even if EAC' adhesion was still high, C3-receptor specific phagocytosis decreased parallel with the amount of the complement used for EA19S sensibilization. The role of receptor sites on human PMN in adhesion and ingestion phases is discussed.

Binding Sites, Antibody↗
PubMed1976

Complement receptors (C3b, C4b/C3d) unbalance on CLL lymphocytes.

A study of lymphocytes bearing C3b, C4b and C3d complement receptor (CRL) was performed on human peripheral blood from 16 healthy donors and 12 patients affected with Chronic Lymphatic Leukemia (CLL). In the latter group a clear rise of C3dCRL was demonstrated, when compared with immunoadherence receptors bearing lymphocytes. However, when compared with controls, also these latter were slightly augmented. Furthermore in CLL under treatment CRL populations showed the same profile as CLL: so it was suggested that the treatment reduced not selectively all the three types of CRL, within the population of sIg bearing lymphocytes. Here we discuss the hypothesis that, in CLL, the proliferating lymphocytes population is chiefly sIg+, C3d+/C3b-, C4b-, but also sIg+, C3d+/C3b+, C4b+.

Animals↗
PubMed1976

Complement profile and anticomplementary potential in mixed cryoglobulinemia.

In six mixed cryoglobulinemias we have found the indications of a complement classic pathway activation and the fall of the total hemolytic activity (CH50): cryoprecipitate anticomplementary power was always proportional to the respective serum CH50 fall, while no correlation with its own immunoglobulin constitution was found.

Animals↗
PubMedDate not supplied

Colloidal gold immunostaining for the detection of platelet reactive allo- and autoantibodies. Use on fresh and frozen cells.

BACKGROUND: Fresh washed platelets are commonly used as target cells for the detection of platelet reactive antibodies. The use of stored platelets would allow a faster execution of the tests and a rapid selection of phenotyped cells. METHODS: Glass-adherent platelet monolayers were freeze stored. The antigen-antibody reaction between platelet antigens and platelet reactive allo- or autoantibodies was revealed by a modified antiglobulin test, using colloidal gold-labeled anti-human IgG. Immunogold staining (IGS) was compared with the platelet suspension immunofluorescence test (PSIFT) and with the solid-phase red cell adherence assay (SPRCA). The reactivity of fresh and freeze-stored platelets was compared. RESULTS: IGS proved to be slightly less sensitive (10%) than PSIFT and SPRCA: The results obtained with fresh and freeze-stored cells were scored identically. CONCLUSIONS: The immunogold staining assay on freeze-stored platelets seems to be a convenient technical approach for platelet serology.

Autoantibodies↗
PubMedDate not supplied

Evidence for the co-existence of immunocomplexes and platelet specific antibodies in HIV infection-related thrombocytopenia in narcotic drug addicts. Experimental results and immunopathogenetic discussion.

BACKGROUND: The development of symptomatic or asymptomatic thrombocytopenia is not uncommon in HIV seropositive drug addicts. METHODS: Platelet binding immunoglobulins, as immune complexes or as platelet specific antibodies, were studied in eleven patients. Whole sera anche serum factions obtained by PEG sedimentation and gel filtration were investigated using methods able to characterize the IgG and IgM immunoglobulin classes. Immune complexes-containing fractions were used in competition assays with some monoclonal antibodies with receptor specificities. RESULTS: Immune complexes able to bind both autologous and allogenic platelets were detected in all patients, while platelet specific IgG or IgM autoantibodies were detected in most, but not in all, patients. The results obtained with the various individual tests were sometimes discrepant, owing to the low avidity binding of platelet-reacting immunoglobulins. Anti-low-affinity FcRII monoclonal antibody moderately reduced the platelet binding of IgG containing immune-complex fractions. CONCLUSIONS: In thrombocytopenic, HIV seropositive drug addicts immunecomplexes and platelet specific antibodies usually coexist and possibly have different pathogenetic potential in the development of thrombocytopenia.

Adult↗
PubMedDate not supplied

ELISA procedures for the characterization of platelet specific antibodies. Evaluation of some variables.

BACKGROUND: Characterization of antiplatelet antibodies is often difficult. METHODS: Four similar ELISA procedures for the characterization of the glycoprotein specificity of antiplatelet antibodies were compared. RESULTS: The four techniques gave overlapping results when tested on thirty-five patients' sera containing antiplatelet antibodies. With ten sera the four procedures gave slightly dissimilar results. All the discrepancies consisted of incomplete glycoprotein identification with partial loss of complete glycoprotein complex recognition. CONCLUSIONS: The analysis of the main technical variables led to the following conclusions: a) in some case antiplatelet antibodies recognize non-glycoproteic moieties of the platelet membrane (glyco- and phospholipids): the negative results found when investigating glycoprotein targets were the correct ones; b) the solubilization of unsensitized glycoproteins possibly leads to conformational changes able to mask the antigenic sites, while previous sensitization confers more stability to the molecule; c) best results were obtained using anti-mouse IgG as capturing agent for sensitized glycoproteins: it is likely that this antibody let a larger number of binding sites available for the second antibody.

Antibodies, Heterophile↗